gltL:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

gltL

Gene Synonym(s)

ECK0645, b0652, JW0647[1], JW0647

Product Desc.

GltL[2][3];

Component of glutamate ABC transporter[2]; GltIJKL glutamate ABC transporter[3]

Glutamate-aspartate transport system ATP-binding protein[4]

Product Synonyms(s)

glutamate and aspartate transporter subunit[1], ATP-binding component of ABC superfamily[1], B0652[2][1], GltL[2][1] , ECK0645, JW0647, b0652

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): gltIJKL[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gltL

Mnemonic

Glutamate

Synonyms

ECK0645, b0652, JW0647[1], JW0647

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

14.74 minutes 

MG1655: 684478..683753
<gbrowseImage> name=NC_000913:683753..684478 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 586513..587238
<gbrowseImage> name=NC_012759:586513..587238 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 685677..684952
<gbrowseImage> name=NC_007779:684952..685677 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 623810..623085
<gbrowseImage> name=NC_010473:623085..623810 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 737070..736345
<gbrowseImage> name=NC_010473:736345..737070 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔgltL (Keio:JW0647)

deletion

deletion

PMID:16738554

Shigen
CGSC8743[5]

gltL::Tn5KAN-2 (FB20218)

Insertion at nt 461 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20218

does not contain pKD46

ΔgltL785::kan

PMID:16738554

CGSC:101208


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0647

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATTACCCTGAAAAATGTTTC

Primer 2:CCATGCAGGATTTTCGCGAGGAA

3A2

Kohara Phage

Genobase

PMID:3038334

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[5]

est. P1 cotransduction: 32% [6]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[5]

est. P1 cotransduction: 55% [6]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12663

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12663

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002351

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945254

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2529

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002232

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GltL

Synonyms

glutamate and aspartate transporter subunit[1], ATP-binding component of ABC superfamily[1], B0652[2][1], GltL[2][1] , ECK0645, JW0647, b0652

Product description

GltL[2][3];

Component of glutamate ABC transporter[2]; GltIJKL glutamate ABC transporter[3]

Glutamate-aspartate transport system ATP-binding protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003439

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017871

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0029

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003439

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017871

F

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of glutamate ABC transporter

could be indirect

Protein

ydcP

PMID:16606699

Experiment(s):EBI-1137604

Protein

yagA

PMID:16606699

Experiment(s):EBI-1137604

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1137604

Protein

yfjI

PMID:16606699

Experiment(s):EBI-1137604

Protein

gltI

PMID:19402753

MALDI(Z-score):39.368125

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MITLKNVSKW YGHFQVLTDC STEVKKGEVV VVCGPSGSGK STLIKTVNGL EPVQQGEITV
DGIVVNDKKT DLAKLRSRVG MVFQHFELFP HLSIIENLTL AQVKVLKRDK APAREKALKL
LERVGLSAHA NKFPAQLSGG QQQRVAIARA LCMDPIAMLF DEPTSALDPE MINEVLDVMV
ELANEGMTMM VVTHEMGFAR KVANRVIFMD EGKIVEDSPK DAFFDDPKSD RAKDFLAKIL H
Length

241

Mol. Wt

26.66 kDa

pI

7.1 (calculated)

Extinction coefficient

6,990 - 7,365 (calc based on 1 Y, 1 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

41..165

PF00005 ABC transporter

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gltL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128635

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945254

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002232

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AAG3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12663

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12663

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945254

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002351

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2529

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

665

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

357

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

492

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

gltIJKL

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:684458..684498 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0652 (EcoliWiki Page)

NCBI GEO profiles for gltL

microarray

GenExpDB:b0652 (EcoliWiki Page)

Summary of data for gltL from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to gltL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12663

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2529

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0652

EcoGene

EcoGene:EG12663

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002351

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002232

Escherichia coli str. K-12 substr. MG1655

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Drosophila melanogaster

  • FBgn0031362 (score: 1.000; bootstrap: 69%)
  • FBgn0032167 (score: 0.229)
  • FBgn0025683 (score: 0.225)
  • FBgn0020762 (score: 0.211)
  • FBgn0031516 (score: 0.208)
  • FBgn0031220 (score: 0.184)
  • FBgn0052091 (score: 0.175)
  • FBgn0031449 (score: 0.159)
  • FBgn0031515 (score: 0.153)
  • FBgn0003996 (score: 0.077)
  • FBgn0003515 (score: 0.074)
  • FBgn0020445 (score: 0.059)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA14594-PA (score: 1.000; bootstrap: 57%)
  • GA16356-PA (score: 0.236)
  • GA15545-PA (score: 0.235)
  • GA19180-PA (score: 0.225)
  • GA18458-PA (score: 0.185)
  • GA16671-PA (score: 0.176)
  • GA16397-PA (score: 0.154)
  • GA15454-PA (score: 0.057)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000130824 (score: 1.000; bootstrap: 57%)
  • NEWSINFRUP00000130826 (score: 0.180)
  • NEWSINFRUP00000167567 (score: 0.137)
  • NEWSINFRUP00000182359 (score: 0.125)
  • NEWSINFRUP00000177047 (score: 0.125)
  • NEWSINFRUP00000168237 (score: 0.097)
  • NEWSINFRUP00000171182 (score: 0.092)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00014344001 (score: 1.000; bootstrap: 57%)
  • GSTENP00014343001 (score: 0.105)
  • GSTENP00020877001 (score: 0.101)
  • GSTENP00038511001 (score: 0.091)
  • GSTENP00014937001 (score: 0.051)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000036735 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

GLTL

From SHIGELLACYC

E. coli O157

GLTL

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00005 ABC transporter

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

EcoCyc

EcoCyc:EG12663

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12663

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002351

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2529

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002232

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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