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Gene |

Product(s) |

Expression|

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Standard Name	gltI
Gene Synonym(s)	ECK0648, b0655, JW5092, ybeJ, yzzK^[1], yzzK
Product Desc.	putative periplasmic binding transport protein^[2]; GltI^[3]; Component of glutamate ABC transporter^[2]; GltIJKL glutamate ABC transporter^[3] Periplasmic glutamate-aspartate binding protein^[4]
Product Synonyms(s)	glutamate and aspartate transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B0655^[2]^[1], YzzK^[2]^[1], YbeJ^[2]^[1], GltI^[2]^[1], GltBP^[3]^[1] , ECK0648, JW5092, ybeJ, yzzK, b0655
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gltIJKL^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gltI
Mnemonic	Glutamate
Synonyms	ECK0648, b0655, JW5092, ybeJ, yzzK^[1], yzzK
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	14.79 minutes	MG1655: 686970..686062 <gbrowseImage> name=NC_000913:686062..686970 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 668979..668071 <gbrowseImage> name=NC_012967:668071..668979 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 588822..589730 <gbrowseImage> name=NC_012759:588822..589730 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 688169..687261 <gbrowseImage> name=NC_007779:687261..688169 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 626302..625394 <gbrowseImage> name=NC_010473:625394..626302 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 739562..738654 <gbrowseImage> name=NC_010473:738654..739562 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	686065	Edman degradation	PMID:8740179 PMID:10972807
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔgltI (Keio:JW5092)	deletion	deletion	PMID:16738554	Shigen CGSC11169^[5]
ΔgltI788::kan			PMID:16738554	CGSC:101202
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5092	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAATTACGTAAACCTGCCAC Primer 2:CCGTTCAGTGCCTTGTCATTCGG
3A2	Kohara Phage	Genobase	PMID:3038334
16A8	Kohara Phage	Genobase	PMID:3038334
crcA280::Tn10	Linked marker	CAG12077 = CGSC7347^[5]	est. P1 cotransduction: 28% ^[6] Synonyms:zbe-280::Tn10
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[5]	est. P1 cotransduction: 60% ^[6] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6359	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12700	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002966	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946938	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2562	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002241	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GltI
Synonyms	glutamate and aspartate transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B0655^[2]^[1], YzzK^[2]^[1], YbeJ^[2]^[1], GltI^[2]^[1], GltBP^[3]^[1] , ECK0648, JW5092, ybeJ, yzzK, b0655
Product description	putative periplasmic binding transport protein^[2]; GltI^[3]; Component of glutamate ABC transporter^[2]; GltIJKL glutamate ABC transporter^[3] Periplasmic glutamate-aspartate binding protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001638	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001638	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0029	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030288	outer membrane-bounded periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001638	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of glutamate ABC transporter	could be indirect
Protein	fur		PMID:19402753	LCMS(ID Probability):99.6
Protein	dapB		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Periplasm		PMID:8740179	EchoLocation:gltI
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQLRKPATAI LALALSAGLA QADDAAPAAG STLDKIAKNG VIVVGHRESS VPFSYYDNQQ KVVGYSQDYS NAIVEAVKKK LNKPDLQVKL IPITSQNRIP LLQNGTFDFE CGSTTNNVER QKQAAFSDTI FVVGTRLLTK KGGDIKDFAN LKDKAVVVTS GTTSEVLLNK LNEEQKMNMR IISAKDHGDS FRTLESGRAV AFMMDDALLA GERAKAKKPD NWEIVGKPQS QEAYGCMLRK DDPQFKKLMD DTIAQVQTSG EAEKWFDKWF KNPIPPKNLN MNFELSDEMK ALFKEPNDKA LN
Length	302
Mol. Wt	33.42 kDa
pI	8.8 (calculated)
Extinction coefficient	23,950 - 24,200 (calc based on 5 Y, 3 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P37902
Domain	42..272	PF00497 Bacterial extracellular solute-binding proteins, family 3		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gltI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128638	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946938	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002241	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37902	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6359	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12700	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946938	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002966	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2562	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.95E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	0.207+/-3.296	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.328193833	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1254	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	3332	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1111	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gltIJKL
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:686950..686990 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0655 (EcoliWiki Page)	NCBI GEO profiles for gltI
microarray	GenExpDB:b0655 (EcoliWiki Page)	Summary of data for gltI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to gltI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6359	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2562	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0655
EcoGene	EcoGene:EG12700	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002966	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002241	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000032943 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000047113 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YBEJ	From SHIGELLACYC
E. coli O157	YBEJ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00497 Bacterial extracellular solute-binding proteins, family 3
Panther (EcoliWiki Page)	PTHR18966:SF42
Superfamily (EcoliWiki Page)	SUPERFAMILY:53850
EcoCyc	EcoCyc:G6359	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12700	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002966	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2562	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002241	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

gltI:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gltI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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