glpT:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpT

Mnemonic

Glycerol phosphate

Synonyms

ECK2232, b2240, JW2234[1], JW2234

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

50.63 minutes 

MG1655: 2350396..2349038
<gbrowseImage> name=NC_000913:2349038..2350396 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2299082..2297724
<gbrowseImage> name=NC_012967:2297724..2299082 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2234843..2236201
<gbrowseImage> name=NC_012759:2234843..2236201 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2357044..2355686
<gbrowseImage> name=NC_007779:2355686..2357044 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2441384..2440026
<gbrowseImage> name=NC_010473:2440026..2441384 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

glpT(del) (Keio:JW2234)

deletion

deletion

PMID:16738554[2]

Shigen

glpT::Tn5KAN-2 (FB20721)

Insertion at nt 822 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB20721

does not contain pKD46

glpT::Tn5KAN-2 (FB20722)

Insertion at nt 822 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB20722

contains pKD46

glpT13

CGSC:10041

glpT6

CGSC:11705

glpt -

Resistant to

Phosphonomycin resistant

PMID:4555418[4]

glpT-(DEL)

Deletion

Deletion

Resistant to

Resistant to Fosfomycin

PMID:363686[5]

Strain: TS104

Parent Strain: TS100

glpT-(DEL)

Deletion

Deletion

Resistant to

Resistant to Fosfomycin

PMID:363686[5]

Strain: LA3430

LA3430 underwent a spontaneous mutation Parent Strain: CSH74

glpT-(DEL)

Point Mutation

Sensitivity to

Sensitive to Fosfomycin

PMID:363686[5]

Strain: LA5004

Parent Strain: TS100

glpT

Resistant to

Resistance to Fosfomycin

PMID:770459[6]

Strain: LA3401

Spontaneously mutated Parent Strain: 72

glpT

Resistant to

Resistance to Fosfomycin

PMID:770459[6]

Strain: LA3402

Spontaneously mutated Parent Strain: 72

glpT

Resistant to

Resistance to Fosfomycin

PMID:770459[6]

Strain: LA3403

Spontaneously mutated Parent Strain: 72

glpT

Resistant to

Resistance to Fosfomycin

PMID:770459[6]

Strain: LA3404

Spontaneously mutated Parent Strain: 72

glpT

Resistant to

Resistance to Fosfomycin

PMID:770459[6]

Strain: LA3405

Spontaneously mutated Parent Strain: 72

glpT

Resistant to

Resistance to Fosfomycin

PMID:217668[7]

Strain: DL39


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2234

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTTGAGTATTTTTAAACCAGC

Primer 2:CCGCCTCCGTTGCGTTCTTGCAG

2E8

Kohara Phage

Genobase

PMID:3038334[9]

22C4

Kohara Phage

Genobase

PMID:3038334[9]

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[10]

est. P1 cotransduction: 82% [11]
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[10]

est. P1 cotransduction: 18% [11]
Synonyms:zej-223::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10401

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10401

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000394

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946704

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0396

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007401

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  4. Venkateswaran, PS & Wu, HC (1972) Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J. Bacteriol. 110 935-44 PubMed
  5. 5.0 5.1 5.2 Argast, M et al. (1978) A second transport system for sn-glycerol-3-phosphate in Escherichia coli. J. Bacteriol. 136 1070-83 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 Silhavy, TJ et al. (1976) Periplasmic protein related to the sn-glycerol-3-phosphate transport system of Escherichia coli. J. Bacteriol. 126 951-8 PubMed
  7. Onaya, T et al. (1978) Evidence for activation by beta2-adrenergic receptors of adenosine 3',5'-monophosphate formation in Ehrlich ascites tumor cells. Endocrinology 103 1122-7 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 CGSC: The Coli Genetics Stock Center
  11. 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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