glpR:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpR

Mnemonic

Glycerol phosphate

Synonyms

ECK3409, b3423, JW3386[1], JW3386

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.68 minutes 

MG1655: 3558628..3557870
<gbrowseImage> name=NC_000913:3557870..3558628 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3489206..3488448
<gbrowseImage> name=NC_012967:3488448..3489206 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3446364..3447122
<gbrowseImage> name=NC_012759:3446364..3447122 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4079810..4080568
<gbrowseImage> name=NC_007779:4079810..4080568 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3656373..3655615
<gbrowseImage> name=NC_010473:3655615..3656373 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3557870

Edman degradation

PMID:8955387[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpR (Keio:JW3386)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10521[4]

glpR::Tn5KAN-I-SceI (FB21194)

Insertion at nt 273 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB21194

does not contain pKD46

glpR2(glpc)

CGSC:4919

glpR12(glpn)

CGSC:6509

glpR8(glpc)

CGSC:6607

glpR7(glpn)

CGSC:6608

glpR200(glpc)

PMID:3882662[6]

CGSC:10441

glpR20(ts)

temperature sensitive

PMID:4866330[7]

CGSC:14471

glpR28

CGSC:14595

glpR5

CGSC:16024

glpR201

CGSC:19381

glpR101

CGSC:71446

ΔglpR756::kan

PMID:16738554[3]

CGSC:101192


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3386

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAACAAACACAACGTCACAA

Primer 2:CCGCACAGCTCCAGTTGAATATG

E5B4

Kohara Phage

Genobase

PMID:3038334[9]

E4E4

Kohara Phage

Genobase

PMID:3038334[9]

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 7% [10]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[4]

est. P1 cotransduction: 84% [10]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10400

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10400

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000393

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947926

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0395

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011176

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Zeng, G et al. (1996) Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain. J. Bacteriol. 178 7080-9 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Elvin, CM et al. (1985) Pi exchange mediated by the GlpT-dependent sn-glycerol-3-phosphate transport system in Escherichia coli. J. Bacteriol. 161 1054-8 PubMed
  7. Cozzarelli, NR et al. (1968) Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J. Mol. Biol. 31 371-87 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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