glpK:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

glpK

Gene Synonym(s)

ECK3918, b3926, JW3897[1], JW3897

Product Desc.

GlpK[2][3];

Component of glycerol kinase[2][3]

Glycerol kinase[4]

Product Synonyms(s)

glycerol kinase[1], B3926[2][1], GlpK[2][1] , ECK3918, JW3897, b3926

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): glpFKX[2], OP00237

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpK

Mnemonic

Glycerol phosphate

Synonyms

ECK3918, b3926, JW3897[1], JW3897

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.66 minutes 

MG1655: 4115245..4113737
<gbrowseImage> name=NC_000913:4113737..4115245 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4096704..4095196
<gbrowseImage> name=NC_012967:4095196..4096704 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4003406..4004914
<gbrowseImage> name=NC_012759:4003406..4004914 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3519459..3520967
<gbrowseImage> name=NC_007779:3519459..3520967 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4214942..4213434
<gbrowseImage> name=NC_010473:4213434..4214942 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4113740

Edman degradation

PMID:2826434


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpK (Keio:JW3897)

deletion

deletion

PMID:16738554

Shigen
CGSC10812[5]

glpKG305S

G305S

In glpK22; abolishes glucose control of glycerol utilization

seeded from UniProt:P0A6F3

glpK16

CGSC:5658

glpK18

CGSC:5813

glpK21

CGSC:5821

glpK22(fbR)

PMID:11344141 PMID:8631672

CGSC:5836

glpK23(p)

CGSC:6514

glpK31(p)

CGSC:7021

glpK1

CGSC:8378

glpK15(fbR)

feedback resistant

CGSC:8929

glpK30

CGSC:9866

glpK4

CGSC:11739

glpK14(fbR)

CGSC:11835

glpK29

CGSC:14607

ΔglpK785::kan

PMID:16738554

CGSC:101188


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3897

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACTGAAAAAAAATATATCGT

Primer 2:CCTTCGTCGTGTTCTTCCCACGC

4H12

Kohara Phage

Genobase

PMID:3038334

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[5]

est. P1 cotransduction: 10% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[5]

est. P1 cotransduction: 32% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10398

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10398

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000391

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948423

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0393

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012825

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GlpK

Synonyms

glycerol kinase[1], B3926[2][1], GlpK[2][1] , ECK3918, JW3897, b3926

Product description

GlpK[2][3];

Component of glycerol kinase[2][3]

Glycerol kinase[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004370

glycerol kinase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00186

F

Seeded from EcoCyc (v14.0)

complete

GO:0004370

glycerol kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005999

F

Seeded from EcoCyc (v14.0)

complete

GO:0004370

glycerol kinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.30

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00186

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000577

P

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018483

P

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018484

P

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018485

P

Seeded from EcoCyc (v14.0)

complete

GO:0006071

glycerol metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0319

P

Seeded from EcoCyc (v14.0)

complete

GO:0006072

glycerol-3-phosphate metabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00186

P

Seeded from EcoCyc (v14.0)

complete

GO:0006072

glycerol-3-phosphate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005999

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000577

F

Seeded from EcoCyc (v14.0)

complete

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018483

F

Seeded from EcoCyc (v14.0)

complete

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018484

F

Seeded from EcoCyc (v14.0)

complete

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018485

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of glycerol kinase

could be indirect

Protein

cysI

PMID:19402753

LCMS(ID Probability):99.6

Protein

motB

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTEKKYIVAL DQGTTSSRAV VMDHDANIIS VSQREFEQIY PKPGWVEHDP MEIWATQSST
LVEVLAKADI SSDQIAAIGI TNQRETTIVW EKETGKPIYN AIVWQCRRTA EICEHLKRDG
LEDYIRSNTG LVIDPYFSGT KVKWILDHVE GSRERARRGE LLFGTVDTWL IWKMTQGRVH
VTDYTNASRT MLFNIHTLDW DDKMLEVLDI PREMLPEVRR SSEVYGQTNI GGKGGTRIPI
SGIAGDQQAA LFGQLCVKEG MAKNTYGTGC FMLMNTGEKA VKSENGLLTT IACGPTGEVN
YALEGAVFMA GASIQWLRDE MKLINDAYDS EYFATKVQNT NGVYVVPAFT GLGAPYWDPY
ARGAIFGLTR GVNANHIIRA TLESIAYQTR DVLEAMQADS GIRLHALRVD GGAVANNFLM
QFQSDILGTR VERPEVREVT ALGAAYLAGL AVGFWQNLDE LQEKAVIERE FRPGIETTER
NYRYAGWKKA VKRAMAWEEH DE
Length

502

Mol. Wt

56.23 kDa

pI

5.3 (calculated)

Extinction coefficient

98,320 - 98,945 (calc based on 18 Y, 13 W, and 5 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6F3

Domain

6..253

PF00370 FGGY family of carbohydrate kinases, N-terminal domain

PMID:19920124

Domain

262..452

PF02782 FGGY family of carbohydrate kinases, C-terminal domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glpK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131764

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948423

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012825

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6F3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10398

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10398

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948423

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000391

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0393

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.16E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

377

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

129

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

172

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

glpFKX

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4115225..4115265 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3926 (EcoliWiki Page)

NCBI GEO profiles for glpK

microarray

GenExpDB:b3926 (EcoliWiki Page)

Summary of data for glpK from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to glpK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10398

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0393

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3926

EcoGene

EcoGene:EG10398

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000391

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012825

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000018137 (score: 1.000; bootstrap: 98%)
  • ENSANGP00000020314 (score: 0.144)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000004685 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT1G80460 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000030898 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000000378 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00037874 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00020007 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000020225 (score: 1.000; bootstrap: 99%)
  • ENSCAFP00000013019 (score: 0.772)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000012436 (score: 1.000; bootstrap: 97%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0218222 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0025592 (score: 1.000; bootstrap: 99%)
  • FBgn0035266 (score: 0.092)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA14911-PA (score: 1.000; bootstrap: 98%)
  • GA20751-PA (score: 0.102)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000026206 (score: 1.000; bootstrap: 95%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000302036 (score: 1.000; bootstrap: 100%)
  • ENSP00000354642 (score: 0.880)
  • ENSP00000350624 (score: 0.830)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000031343 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000022823 (score: 0.802)
  • ENSMMUP00000007837 (score: 0.571)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000018135 (score: 1.000; bootstrap: 99%)
  • ENSMODP00000002944 (score: 0.828)

From Inparanoid:20070104

Mus musculus

  • MGI:106594 (score: 1.000; bootstrap: 99%)
  • MGI:1329027 (score: 0.767)
  • MGI:891990 (score: 0.744)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000040915 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000028445 (score: 0.784)
  • ENSPTRP00000037327 (score: 0.237)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000045775 (score: 1.000; bootstrap: 99%)
  • ENSRNOP00000047647 (score: 0.714)
  • ENSRNOP00000043080 (score: 0.630)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YHL032C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCPJ7322c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000163684 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000136236 (score: 0.109)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00033107001 (score: 1.000; bootstrap: 100%)
  • GSTENP00019440001 (score: 0.198)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000040298 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Shigella flexneri

GLPK

From SHIGELLACYC

E. coli O157

GLPK

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00370 FGGY family of carbohydrate kinases, N-terminal domain

Pfam (EcoliWiki Page)

PF02782 FGGY family of carbohydrate kinases, C-terminal domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:53067

Superfamily (EcoliWiki Page)

SUPERFAMILY:53067

Panther (EcoliWiki Page)

PTHR10196:SF9

EcoCyc

EcoCyc:EG10398

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10398

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000391

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0393

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012825

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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