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Standard Name	glnV
Gene Synonym(s)	ECK0656, b0665, JWR0012, Su2, Su_II, supE, glnValpha, suII^[1], suII
Product Desc.
Product Synonyms(s)	tRNA-Gln(CUG) (Glutamine tRNA2)^[1]
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): metT-leuW-glnUW-metU-glnVX^[2], metT
Regulation/Activity
Quick Links
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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	glnV
Mnemonic	Glutamine
Synonyms	ECK0656, b0665, JWR0012, Su2, Su_II, supE, glnValpha, suII^[1], suII
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	15 minutes	MG1655: 695839..695765 <gbrowseImage> name=NC_000913:695765..695839 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 678637..678711 <gbrowseImage> name=NC_012967:678637..678711 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 598525..598599 <gbrowseImage> name=NC_012759:598525..598599 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 697038..696964 <gbrowseImage> name=NC_007779:696964..697038 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 748431..748357 <gbrowseImage> name=NC_010473:748357..748431 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
glnV42(AS)			amber (UAG) suppressor		CGSC:4563
glnV44(AS)			amber (UAG) suppressor	PMID:5331503	CGSC:4497
glnV0(AS)			amber (UAG) suppressor		CGSC:10415
glnV33(AS)			amber (UAG) suppressor		CGSC:11602
glnV22(AS)			amber (UAG) suppressor		CGSC:13836
glnV41(AS)			amber (UAG) suppressor		CGSC:13854
glnV93(AS)			amber (UAG) suppressor		CGSC:27776
glnV89(AS)			amber (UAG) suppressor		CGSC:74381
glnV44 rpsL-asuB3	Insertion of anti-suppressor genes.	Efficiency of Suppressor	Decrease in suppressor efficiency	PMID:3918006		See in Table 5 for suppression efficiency results.
glnV44 rpsL-asuB3	Insertion of anti-suppressor genes.	Efficiency of Suppressor	Decrease in suppressor efficiency	PMID:3918006		See in Table 5 for suppression efficiency results.
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Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
16A8	Kohara Phage	Genobase	PMID:3038334
crcA280::Tn10	Linked marker	CAG12077 = CGSC7347^[3]	est. P1 cotransduction: 17% ^[4] Synonyms:zbe-280::Tn10
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[3]	est. P1 cotransduction: 85% ^[4] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG30029	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG30029	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002471	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945255	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4192	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002274	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	tRNA-Gln(CUG) (Glutamine tRNA2)
Synonyms	tRNA-Gln(CUG) (Glutamine tRNA2)^[1]
Product description
EC number (for enzymes)
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Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

supE44 (synonymous to glnV) acts as an amber nonsense mutation (insertion of UAG stop codon) suppressor. This tRNA structural gene mutation, first isolated by Bachmann et al., 1976, causes the transition of the GUC glutamine anticodon to a AUC anticodon. The mutant SupE44 tRNA competes with the amber nonsense mutation tRNA in translation, yielding a phenotype with lower levels of termination of translation.

supE44 's phenotype was determined by Inokuchi et al., 1979. Upon infection with mutant phage containing supE44, higher levels of tRNA^Gln₂ were noted; nuclease digestion and gel analysis yielded similar results to Yaniv and Folk's (1975) tRNA^Gln₂ sequences.

See Bachmann et al., 1976, ^[5]; Inokuchi et al., 1979, ^[6]; and Yaniv and Folk, 1975, ^[7].

Last updated 11/11/07 by user TPian.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties

Type	tRNA
Sequence	UGGGGUAUCG CCAAGCGGUA AGGCACCGGA UUCUGAUUCC GGCAUUCCGA GGUUCGAAUC CUCGUACCCC AGCCA
Mol. Wt	24264.42
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Domains/Motifs/Modification Sites See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map />

Image couresy of Rfam

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glnV taxon=562,83333 </beststructure>

Models

View models at:

PSI-PRED
Genomes TO Protein (GTOP)

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG30029	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG30029	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002471	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4192	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002274	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Transcription and Transcriptional Regulation

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Transcription unit(s)
metT-leuW-glnUW-metU-glnVX
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:695819..695859 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0665 (EcoliWiki Page)	NCBI GEO profiles for glnV
microarray	GenExpDB:b0665 (EcoliWiki Page)	Summary of data for glnV from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to glnV Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG30029	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4192	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0665
EcoGene	EcoGene:EG30029	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002471	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002274	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
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Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF01360
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Families

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Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 CGSC: The Coli Genetics Stock Center
↑ ^4.0 ^4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Bachmann, BJ et al. (1976) Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev 40 116-67 PubMed
↑ Inokuchi, H et al. (1979) Identification of transfer RNA suppressors in Escherichia coli. II. Duplicate genes for tRNA2Gln. J. Mol. Biol. 132 663-77 PubMed
↑ Yaniv, M & Folk, WR (1975) The nucleotide sequences of the two glutamine transfer ribonucleic acids from Escherichia coli. J. Biol. Chem. 250 3243-53 PubMed

glnV:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to glnV Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Families

Notes

Links

References

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