glnV:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

tRNA-Gln(CUG) (Glutamine tRNA2)

Synonyms

tRNA-Gln(CUG) (Glutamine tRNA2)[1]

Product description


EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
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Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

supE44 (synonymous to glnV) acts as an amber nonsense mutation (insertion of UAG stop codon) suppressor. This tRNA structural gene mutation, first isolated by Bachmann et al., 1976, causes the transition of the GUC glutamine anticodon to a AUC anticodon. The mutant SupE44 tRNA competes with the amber nonsense mutation tRNA in translation, yielding a phenotype with lower levels of termination of translation.

supE44 's phenotype was determined by Inokuchi et al., 1979. Upon infection with mutant phage containing supE44, higher levels of tRNAGln2 were noted; nuclease digestion and gel analysis yielded similar results to Yaniv and Folk's (1975) tRNAGln2 sequences.

See Bachmann et al., 1976, [2]; Inokuchi et al., 1979, [3]; and Yaniv and Folk, 1975, [4].

Last updated 11/11/07 by user TPian.

Localization

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Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties

Type

tRNA

Sequence
UGGGGUAUCG CCAAGCGGUA AGGCACCGGA UUCUGAUUCC GGCAUUCCGA GGUUCGAAUC
CUCGUACCCC AGCCA
Mol. Wt

24264.42


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Type Residues Description Notes References

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Image couresy of Rfam


Structure
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Structures

<beststructure> gene=glnV taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc

EcoCyc:EG30029

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG30029

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002471

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4192

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002274

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Bachmann, BJ et al. (1976) Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev 40 116-67 PubMed
  3. Inokuchi, H et al. (1979) Identification of transfer RNA suppressors in Escherichia coli. II. Duplicate genes for tRNA2Gln. J. Mol. Biol. 132 663-77 PubMed
  4. Yaniv, M & Folk, WR (1975) The nucleotide sequences of the two glutamine transfer ribonucleic acids from Escherichia coli. J. Biol. Chem. 250 3243-53 PubMed

Categories

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