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Standard Name	glnL
Gene Synonym(s)	ECK3862, b3869, JW3840, GlnC, glnR, ntrB, G256^[1]^[2]
Product Desc.	NtrB^[2]^[3]; Component of NtrB^[2]^[3] Bifunctional protein kinase/phosphatase, nitrogen regulator II, NRII; homodimeric^[4]
Product Synonyms(s)	sensory histidine kinase in two-component regulatory system with GlnG^[1], B3869^[2]^[1], NRII^[2]^[1], GlnR^[2]^[1], GlnL^[2]^[1], kinase-phosphotransferase nitrogen regulator II^[2]^[1], unphosphorylated kinase-phosphotransferase nitrogen regulator II^[2]^[1], unmodified kinase-phosphotransferase nitrogen regulator II^[2]^[1] , ECK3862, glnR, JW3840, ntrB, b3869
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): glnALG^[2], OP00034, glnLG^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	glnL
Mnemonic	Glutamine
Synonyms	ECK3862, b3869, JW3840, GlnC, glnR, ntrB, G256^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	87.36 minutes	MG1655: 4054362..4053313 <gbrowseImage> name=NC_000913:4053313..4054362 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4034597..4033548 <gbrowseImage> name=NC_012967:4033548..4034597 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3942982..3944031 <gbrowseImage> name=NC_012759:3942982..3944031 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3580342..3581391 <gbrowseImage> name=NC_007779:3580342..3581391 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4153282..4152233 <gbrowseImage> name=NC_010473:4152233..4153282 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4053316	Edman degradation	PMID:2016302
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔglnL (Keio:JW3840)	deletion	deletion	PMID:16738554	Shigen CGSC10774^[5]
ΔglnL2001			PMID:2865248	CGSC:76312
ΔglnL731::kan			PMID:16738554	CGSC:101166
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3840	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAACAGGCACGCAGCCCGA Primer 2:CCTTTCCTGATAGGCAGGTAAAC
10F1	Kohara Phage	Genobase	PMID:3038334
8D12	Kohara Phage	Genobase	PMID:3038334
fad-751::Tn10	Linked marker	CAG18496 = CGSC7465^[5]	est. P1 cotransduction: 37% ^[6] Synonyms:fadAB101::Tn10
zih-35::Tn10	Linked marker	CAG18495 = CGSC7467^[5]	est. P1 cotransduction: 68% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10387	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10387	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000380	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948360	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0382	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012634	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GlnL
Synonyms	sensory histidine kinase in two-component regulatory system with GlnG^[1], B3869^[2]^[1], NRII^[2]^[1], GlnR^[2]^[1], GlnL^[2]^[1], kinase-phosphotransferase nitrogen regulator II^[2]^[1], unphosphorylated kinase-phosphotransferase nitrogen regulator II^[2]^[1], unmodified kinase-phosphotransferase nitrogen regulator II^[2]^[1] , ECK3862, glnR, JW3840, ntrB, b3869
Product description	NtrB^[2]^[3]; Component of NtrB^[2]^[3] Bifunctional protein kinase/phosphatase, nitrogen regulator II, NRII; homodimeric^[4]
EC number (for enzymes)
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Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of NtrB	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MATGTQPDAG QILNSLINSI LLIDDNLAIH YANPAAQQLL AQSSRKLFGT PLPELLSYFS LNIELMQESL EAGQGFTDNE VTLVIDGRSH ILSVTAQRMP DGMILLEMAP MDNQRRLSQE QLQHAQQVAA RDLVRGLAHE IKNPLGGLRG AAQLLSKALP DPSLLEYTKV IIEQADRLRN LVDRLLGPQL PGTRVTESIH KVAERVVTLV SMELPDNVRL IRDYDPSLPE LAHDPDQIEQ VLLNIVRNAL QALGPEGGEI ILRTRTAFQL TLHGERYRLA ARIDVEDNGP GIPPHLQDTL FYPMVSGREG GTGLGLSIAR NLIDQHSGKI EFTSWPGHTE FSVYLPIRK
Length	349
Mol. Wt	38.557 kDa
pI	5.5 (calculated)
Extinction coefficient	15,930 (calc based on 7 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	233..348	PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase	PMID:19920124
Domain	129..189	PF00512 His Kinase A (phosphoacceptor) domain	PMID:19920124
Domain	10..108	PF00989 PAS fold	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glnL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131709	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948360	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012634	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFB5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10387	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10387	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948360	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000380	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0382	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.86E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	27.489+/-0.235	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.054814109	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	54	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	82	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	24^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
glnALG
glnLG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4054342..4054382 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3869 (EcoliWiki Page)	NCBI GEO profiles for glnL
microarray	GenExpDB:b3869 (EcoliWiki Page)	Summary of data for glnL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4053821..4054252) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ01; Well:F7^[7]
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Notes

Accessions Related to glnL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10387	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0382	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3869
EcoGene	EcoGene:EG10387	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000380	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012634	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000133 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT4G18130 (score: 1.000; bootstrap: 100%) AT2G18790 (score: 0.549) AT4G16250 (score: 0.524) AT1G09570 (score: 0.438) AT5G35840 (score: 0.432)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0215354 (score: 1.000; bootstrap: 100%) DDB0220020 (score: 0.090) DDB0215358 (score: 0.079)	From Inparanoid:20070104
Shigella flexneri	GLNL	From SHIGELLACYC
E. coli O157	GLNL	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00512 His Kinase A (phosphoacceptor) domain
Pfam (EcoliWiki Page)	PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Superfamily (EcoliWiki Page)	SUPERFAMILY:47384
Panther (EcoliWiki Page)	PTHR26402:SF24
Superfamily (EcoliWiki Page)	SUPERFAMILY:55785
Superfamily (EcoliWiki Page)	SUPERFAMILY:55874
Pfam (EcoliWiki Page)	PF00989 PAS fold
EcoCyc	EcoCyc:EG10387	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10387	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000380	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0382	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012634	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 ^2.21 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

glnL:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to glnL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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