glmU:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

glmU

Gene Synonym(s)

ECK3723, b3730, JW3708, yieA, tms(B.s.)[1][2], tms

Product Desc.

GlmU[2][3];

Component of NAG1P-URIDYLTRANS-CPLX[2]

Bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase[4]

Product Synonyms(s)

fused N-acetyl glucosamine-1-phosphate uridyltransferase[1], glucosamine-1-phosphate acetyl transferase[1], B3730[2][1], YieA[2][1], GlmU[2][1] , ECK3723, JW3708, tms, yieA, b3730

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): glmUS[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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GlmU contains 22 hexapeptide repeats. Binds TrxA (Kumar, 2004). HT_Cmplx21_Cyt: GlmU+Pta.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glmU

Mnemonic

Glucosamine

Synonyms

ECK3723, b3730, JW3708, yieA, tms(B.s.)[1][2], tms

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.31 minutes 

MG1655: 3913223..3911853
<gbrowseImage> name=NC_000913:3911853..3913223 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3875566..3874196
<gbrowseImage> name=NC_012967:3874196..3875566 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3800186..3801556
<gbrowseImage> name=NC_012759:3800186..3801556 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3721481..3722851
<gbrowseImage> name=NC_007779:3721481..3722851 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4010807..4009437
<gbrowseImage> name=NC_010473:4009437..4010807 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

glmUR18A

R18A

Dramatically impairs the uridyltransferase activity

seeded from UniProt:P0ACC7

glmUK25A

K25A

8-fold decrease in uridyltransferase activity

seeded from UniProt:P0ACC7

glmUC296A

C296A

No effect

seeded from UniProt:P0ACC7

glmUC307A

C307A

1350-fold decrease in acetyltransferase activity

seeded from UniProt:P0ACC7

glmUC324A

C324A

8-fold decrease in acetyltransferase activity

seeded from UniProt:P0ACC7

glmUC385A

C385A

No effect

seeded from UniProt:P0ACC7

glmUG14A

G14A

8-fold decrease in uridyltransferase activity. Creates steric conflict and decreases affinity for UTP

seeded from UniProt:P0ACC7

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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3708

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTTGAATAATGCTATGAGCGT

Primer 2:CCtTTTTTCTTTACCGGACGACG

2A1

Kohara Phage

Genobase

PMID:3038334

7A1

Kohara Phage

Genobase

PMID:3038334

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[5]

est. P1 cotransduction: 21% [6]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[5]

est. P1 cotransduction: 52% [6]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11198

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11198

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001179

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948246

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1184

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012201

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GlmU

Synonyms

fused N-acetyl glucosamine-1-phosphate uridyltransferase[1], glucosamine-1-phosphate acetyl transferase[1], B3730[2][1], YieA[2][1], GlmU[2][1] , ECK3723, JW3708, tms, yieA, b3730

Product description

GlmU[2][3];

Component of NAG1P-URIDYLTRANS-CPLX[2]

Bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01631

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0000902

cell morphogenesis

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01631

P

Seeded from EcoCyc (v14.0)

complete

GO:0000902

cell morphogenesis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

P

Seeded from EcoCyc (v14.0)

complete

GO:0003977

UDP-N-acetylglucosamine diphosphorylase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01631

F

Seeded from EcoCyc (v14.0)

complete

GO:0003977

UDP-N-acetylglucosamine diphosphorylase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

F

Seeded from EcoCyc (v14.0)

complete

GO:0003977

UDP-N-acetylglucosamine diphosphorylase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.23

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01631

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0007047

cellular cell wall organization

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0961

P

Seeded from EcoCyc (v14.0)

complete

GO:0008360

regulation of cell shape

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0133

P

Seeded from EcoCyc (v14.0)

complete

GO:0009103

lipopolysaccharide biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01631

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0573

P

Seeded from EcoCyc (v14.0)

complete

GO:0019134

glucosamine-1-phosphate N-acetyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01631

F

Seeded from EcoCyc (v14.0)

complete

GO:0019134

glucosamine-1-phosphate N-acetyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005882

F

Seeded from EcoCyc (v14.0)

complete

GO:0019134

glucosamine-1-phosphate N-acetyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.3.1.157

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0050897

cobalt ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0170

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of NAG1P-URIDYLTRANS-CPLX

could be indirect

Protein

rpsB

PMID:15690043

Experiment(s):EBI-878861

Protein

rplO

PMID:16606699

Experiment(s):EBI-1146586

Protein

rpsC

PMID:16606699

Experiment(s):EBI-1146586

Protein

rpsG

PMID:16606699

Experiment(s):EBI-1146586

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1146586

Protein

ulaF

PMID:16606699

Experiment(s):EBI-1146586

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1146586

Protein

fur

PMID:16606699

Experiment(s):EBI-1146586

Protein

yafO

PMID:16606699

Experiment(s):EBI-1146586

Protein

rpsE

PMID:16606699

Experiment(s):EBI-1146586

Protein

rplB

PMID:16606699

Experiment(s):EBI-1146586

Protein

rplA

PMID:16606699

Experiment(s):EBI-1146586

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1146586

Protein

yjfM

PMID:16606699

Experiment(s):EBI-1146586

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MLNNAMSVVI LAAGKGTRMY SDLPKVLHTL AGKAMVQHVI DAANELGAAH VHLVYGHGGD
LLKQALKDDN LNWVLQAEQL GTGHAMQQAA PFFADDEDIL MLYGDVPLIS VETLQRLRDA
KPQGGIGLLT VKLDDPTGYG RITRENGKVT GIVEHKDATD EQRQIQEINT GILIANGADM
KRWLAKLTNN NAQGEYYITD IIALAYQEGR EIVAVHPQRL SEVEGVNNRL QLSRLERVYQ
SEQAEKLLLA GVMLRDPARF DLRGTLTHGR DVEIDTNVII EGNVTLGHRV KIGTGCVIKN
SVIGDDCEIS PYTVVEDANL AAACTIGPFA RLRPGAELLE GAHVGNFVEM KKARLGKGSK
AGHLTYLGDA EIGDNVNIGA GTITCNYDGA NKFKTIIGDD VFVGSDTQLV APVTVGKGAT
IAAGTTVTRN VGENALAISR VPQTQKEGWR RPVKKK
Length

456

Mol. Wt

49.19 kDa

pI

6.5 (calculated)

Extinction coefficient

32,890 - 33,390 (calc based on 11 Y, 3 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

7..220

PF00483 Nucleotidyl transferase

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glmU taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131598

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948246

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012201

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACC7

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11198

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11198

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948246

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001179

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1184

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

7.37E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

42.07+/-0.406

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.152554745

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

3249

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

795

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2575

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

glmUS

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3913203..3913243 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3730 (EcoliWiki Page)

NCBI GEO profiles for glmU

microarray

GenExpDB:b3730 (EcoliWiki Page)

Summary of data for glmU from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3912729..3913198) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ04; Well:C7[7]

<protect></protect>

Notes

Accessions Related to glmU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11198

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1184

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3730

EcoGene

EcoGene:EG11198

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001179

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012201

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Oryza gramene

  • Q6Z9A3 (score: 1.000; bootstrap: 100%)
  • Q941T9 (score: 0.983)
  • Q84JH5 (score: 0.902)
  • Q8H057 (score: 0.120)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000043430 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

GLMU

From SHIGELLACYC

E. coli O157

GLMU

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00483 Nucleotidyl transferase

Superfamily (EcoliWiki Page)

SUPERFAMILY:51161

Superfamily (EcoliWiki Page)

SUPERFAMILY:53448

Panther (EcoliWiki Page)

PTHR22572:SF17

EcoCyc

EcoCyc:EG11198

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11198

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001179

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1184

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012201

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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