glgB:On One Page
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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| Standard Name |
glgB |
|---|---|
| Gene Synonym(s) |
ECK3418, b3432, JW3395[1], JW3395 |
| Product Desc. |
GLYCOGEN- 1,4-alpha-glucan branching enzyme; glycogen branching enzyme[2] |
| Product Synonyms(s) |
1,4-alpha-glucan branching enzyme[1], B3432[3][1], GlgB[3][1] , ECK3418, JW3395, b3432 |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links |
|
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
glgB |
|---|---|
| Mnemonic |
Glycogen |
| Synonyms |
ECK3418, b3432, JW3395[1], JW3395 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
76.93 minutes |
MG1655: 3571525..3569339 |
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|
NC_012967: 3504956..3502770 |
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NC_012759: 3457833..3460019 |
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|
W3110 |
|
W3110: 4066913..4069099 |
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|
DH10B: 3669270..3667084 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔglgB (Keio:JW3395) |
deletion |
deletion |
PMID:16738554 |
||||
|
glgB::Tn5KAN-I-SceI (FB21203) |
Insertion at nt 1255 in Minus orientation |
PMID:15262929 |
contains pKD46 | ||||
|
glgB2 |
|||||||
|
ΔglgB765::kan |
PMID:16738554 |
| |||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3395 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCTCCGATCGTATCGATAGAGA Primer 2:CCTTCTGCCTCCCGAACCAGCCA | |
|
Kohara Phage |
PMID:3038334 | ||
|
Kohara Phage |
PMID:3038334 | ||
|
Linked marker |
est. P1 cotransduction: 82% [6] | ||
|
zhg-50::Tn10 |
Linked marker |
est. P1 cotransduction: 45% [6] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120000371 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EchoBASE:EB0373 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0011209 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
GlgB |
|---|---|
| Synonyms |
1,4-alpha-glucan branching enzyme[1], B3432[3][1], GlgB[3][1] , ECK3418, JW3395, b3432 |
| Product description |
GLYCOGEN- 1,4-alpha-glucan branching enzyme; glycogen branching enzyme[2] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
GO:0000271 |
polysaccharide biosynthetic process |
P |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
|
GO:0003844 |
1,4-alpha-glucan branching enzyme activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00685 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0003844 |
1,4-alpha-glucan branching enzyme activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006407 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0003844 |
1,4-alpha-glucan branching enzyme activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:2.4.1.18 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0004553 |
hydrolase activity, hydrolyzing O-glycosyl compounds |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004193 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0005978 |
glycogen biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00685 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0005978 |
glycogen biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006407 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0005978 |
glycogen biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0320 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0043169 |
cation binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006047 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0043169 |
cation binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006048 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0043169 |
cation binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013780 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0043169 |
cation binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013781 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0006974 |
response to DNA damage stimulus |
PMID:11967071 |
IEP: Inferred from Expression Pattern |
P |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
ygcL |
PMID:16606699 |
Experiment(s):EBI-1145888 | |
|
Protein |
groL |
PMID:16606699 |
Experiment(s):EBI-1145888 | |
|
Protein |
norV |
PMID:16606699 |
Experiment(s):EBI-1145888 | |
|
Protein |
ybfO |
PMID:16606699 |
Experiment(s):EBI-1145888 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE PKTGRKLAKL ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF SVWAPNARRV SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID ALRVDAVASM IYRDYSRKEG EWIPNEFGGR ENLEAIEFLR NTNRILGEQV SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL DYMKLDPVYR QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD EIASHGRQHS LSLTLPPLAT IWLVREAE |
| Length |
728 |
| Mol. Wt |
84.338 kDa |
| pI |
6.2 (calculated) |
| Extinction coefficient |
190,680 - 190,930 (calc based on 32 Y, 26 W, and 2 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
ASAP:ABE-0011209 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EcoCyc:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB:ECK120000371 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB0373 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
E. coli K-12 MC4100 |
9.99E+01 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
|
Protein |
E. coli K-12 MG1655 |
117 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
448 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
239 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
| edit table |
Notes
Transcription and Transcriptional Regulation
<protect>
|
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
 Figure courtesy of RegulonDB |
</protect>
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:3571505..3571545
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
</protect>
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for glgB | |
|
microarray |
Summary of data for glgB from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
|
GFP Fusion |
Intergenic region (3571036..3571469) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
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Notes
Accessions Related to glgB Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB0373 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120000371 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0011209 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
| Organism | Homologs (Statistics) | Comments |
|---|---|---|
|
Anopheles gambiae |
|
From Inparanoid:20070104 |
|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
|
Bos taurus |
|
From Inparanoid:20070104 |
|
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
|
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
|
Canis familiaris |
|
From Inparanoid:20070104 |
|
Ciona intestinalis |
|
From Inparanoid:20070104 |
|
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
|
Drosophila melanogaster |
|
From Inparanoid:20070104 |
|
Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
|
Gallus gallus |
|
From Inparanoid:20070104 |
|
Homo sapiens |
|
From Inparanoid:20070104 |
|
Macaca mulatta |
|
From Inparanoid:20070104 |
|
Monodelphis domestica |
|
From Inparanoid:20070104 |
|
Mus musculus |
|
From Inparanoid:20070104 |
|
Oryza gramene |
|
From Inparanoid:20070104 |
|
Pan troglodytes |
|
From Inparanoid:20070104 |
|
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
|
Takifugu rubripes |
|
From Inparanoid:20070104 |
|
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
|
Xenopus tropicalis |
|
From Inparanoid:20070104 |
|
Shigella flexneri |
GLGB |
From SHIGELLACYC |
|
E. coli O157 |
GLGB |
From ECOO157CYC |
| edit table |
Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
PF02922 Carbohydrate-binding module 48 (Isoamylase N-terminal domain) |
||
|
EcoCyc:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG10378 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120000371 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB0373 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0011209 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
Categories
- Genes in OpenBioSystems with Promoter Fusions
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Dictyostelium discoideum
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Drosophila pseudoobscura
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Oryza gramene
- Genes with homologs in Pan troglodytes
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157
