PMID:17805344
| Citation |
Grainge, I, Bregu, M, Vazquez, M, Sivanathan, V, Ip, SC and Sherratt, DJ (2007) Unlinking chromosome catenanes in vivo by site-specific recombination. EMBO J. 26:4228-38 |
|---|---|
| Abstract |
A challenge for chromosome segregation in all domains of life is the formation of catenated progeny chromosomes, which arise during replication as a consequence of the interwound strands of the DNA double helix. Topoisomerases play a key role in DNA unlinking both during and at the completion of replication. Here we report that chromosome unlinking can instead be accomplished by multiple rounds of site-specific recombination. We show that step-wise, site-specific recombination by XerCD-dif or Cre-loxP can unlink bacterial chromosomes in vivo, in reactions that require KOPS-guided DNA translocation by FtsK. Furthermore, we show that overexpression of a cytoplasmic FtsK derivative is sufficient to allow chromosome unlinking by XerCD-dif recombination when either subunit of TopoIV is inactivated. We conclude that FtsK acts in vivo to simplify chromosomal topology as Xer recombination interconverts monomeric and dimeric chromosomes. |
| Links |
PubMed PMC2230843 Online version:10.1038/sj.emboj.7601849 |
| Keywords |
Chromosomes, Bacterial/genetics; Chromosomes, Bacterial/metabolism; DNA Replication/physiology; DNA Topoisomerase IV/genetics; DNA Topoisomerase IV/metabolism; DNA, Catenated/genetics; DNA, Catenated/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Integrases/genetics; Integrases/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Recombination, Genetic/physiology |
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