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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	ftsI
Gene Synonym(s)	ECK0085, b0084, JW0082, pbpB, sep^[1], sep
Product Desc.	essential cell division protein FtsI; penicillin-binding protein 3^[2]^[3] Transpetidase, PBP3; penicillin-binding protein 3 involved in septal peptidoglycan synthesis^[4]
Product Synonyms(s)	transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3)^[1], B0084^[2]^[1], Sep^[2]^[1], PbpB^[2]^[1], FtsI^[2]^[1], PBP3^[2]^[1] , ECK0085, JW0082, pbpB, sep, b0084
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ^[2]
Regulation/Activity
Quick Links
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Notes

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FtsI is recruited by its cognate SEDS protein FtsW. Essential gene, Hara (1997). FtsI was reported to be a lipoprotein (Hayashi, 1989), but only 15% of the protein was modified. The physiological significance of this is unknown. The sequence is not predicted to be a lipoprotein cleavage site. Divisome.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ftsI
Mnemonic	Filamentation, temperature sensitive
Synonyms	ECK0085, b0084, JW0082, pbpB, sep^[1], sep
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	1.97 minutes	MG1655: 91413..93179 <gbrowseImage> name=NC_000913:91413..93179 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 94217..95983 <gbrowseImage> name=NC_012967:94217..95983 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 91412..93178 <gbrowseImage> name=NC_012759:91412..93178 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 91413..93179 <gbrowseImage> name=NC_007779:91413..93179 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 65517..67283 <gbrowseImage> name=NC_010473:65517..67283 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ftsIS307A,T		S307A,T		Unable to bind penicillin			seeded from UniProt:P0AD68
ftsIS307C		S307C		Still able to bind penicillin			seeded from UniProt:P0AD68
ftsIN361S		N361S		In PBPBR1; obtained after selection for increased resistance to cephalexin, causes a change in the shape of the cell: The polar caps are pointed			seeded from UniProt:P0AD68
ftsI23(ts)	TAC to GAC	Y380D	Growth Phenotype	at non-permissive temperature filaments with indented morphology were formed	PMID:8682793	CGSC:91088
ftsI2158(ts)			Growth Phenotype	filaments form at 42C	PMID:4591963 PMID:338600	CGSC:10211	part of AX655 strain
pbpB2158				temperature shift to 42 C caused blunt constrictions	PMID:3280547		a shift back to permissive temperature results in aberrant constrictions and formation of minicell-like cells
ftsI372 (Ts)		Pro-372 to Leu	Growth Phenotype	after 2 hours at 42C filamentation occurs and amount of PMP3 was significantly reduced	PMID:1551860
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0082	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAGCAGCGGCGAAAACGCA Primer 2:CCCGATCTGCCACCTGTCCCCTC
6C1	Kohara Phage	Genobase	PMID:3038334
6F3	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 77% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 6% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10341	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10341	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000335	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944799	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0337	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000309	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FtsI
Synonyms	transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3)^[1], B0084^[2]^[1], Sep^[2]^[1], PbpB^[2]^[1], FtsI^[2]^[1], PBP3^[2]^[1] , ECK0085, JW0082, pbpB, sep, b0084
Product description	essential cell division protein FtsI; penicillin-binding protein 3^[2]^[3] Transpetidase, PBP3; penicillin-binding protein 3 involved in septal peptidoglycan synthesis^[4]
EC number (for enzymes)	2.4.1.129^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008955	peptidoglycan glycosyltransferase activity	PMID:6450748	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0008955	peptidoglycan glycosyltransferase activity	PMID:7030331	IDA: Inferred from Direct Assay		F		complete
	GO:0031226	intrinsic to plasma membrane	PMID:3881388	IDA: Inferred from Direct Assay		C		complete
	GO:0005887	integral to plasma membrane	PMID:319999	IDA: Inferred from Direct Assay		C		complete
	GO:0005515	protein binding	PMID:18165305	IGI: Inferred from Genetic Interaction		F	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0007047	cellular cell wall organization	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0961	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0131	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0133	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008658	penicillin binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008658	penicillin binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005311	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008955	peptidoglycan glycosyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.4.1.129	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009252	peptidoglycan biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0573	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009273	peptidoglycan-based cell wall biogenesis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001460	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0132	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:20497333	IPI: Inferred from Physical Interaction	EcoliWiki:ftsN	F		complete
	GO:0032155	cell division site part	PMID:9603865	IDA: Inferred from Direct Assay		C		complete
	GO:0005515	protein binding	PMID:18165305	IGI: Inferred from Genetic Interaction	EcoliWiki:mtgA	F		complete
	GO:0051301	cell division	PMID:1103132	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0008658	penicillin binding	PMID:1103132	IDA: Inferred from Direct Assay		F		complete
	GO:0042493	response to drug	PMID:1103132	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKAAAKTQKP KRQEEHANFI SWRFALLCGC ILLALAFLLG RVAWLQVISP DMLVKEGDMR SLRVQQVSTS RGMITDRSGR PLAVSVPVKA IWADPKEVHD AGGISVGDRW KALANALNIP LDQLSARINA NPKGRFIYLA RQVNPDMADY IKKLKLPGIH LREESRRYYP SGEVTAHLIG FTNVDSQGIE GVEKSFDKWL TGQPGERIVR KDRYGRVIED ISSTDSQAAH NLALSIDERL QALVYRELNN AVAFNKAESG SAVLVDVNTG EVLAMANSPS YNPNNLSGTP KEAMRNRTIT DVFEPGSTVK PMVVMTALQR GVVRENSVLN TIPYRINGHE IKDVARYSEL TLTGVLQKSS NVGVSKLALA MPSSALVDTY SRFGLGKATN LGLVGERSGL YPQKQRWSDI ERATFSFGYG LMVTPLQLAR VYATIGSYGI YRPLSITKVD PPVPGERVFP ESIVRTVVHM MESVALPGGG GVKAAIKGYR IAIKTGTAKK VGPDGRYINK YIAYTAGVAP ASQPRFALVV VINDPQAGKY YGGAVSAPVF GAIMGGVLRT MNIEPDALTT GDKNEFVINQ GEGTGGRS
Length	588
Mol. Wt	63.877 kDa
pI	10.0 (calculated)
Extinction coefficient	64,290 - 64,540 (calc based on 21 Y, 6 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	260..555	PF00905 Penicillin binding protein transpeptidase domain	PMID:19920124
Domain	66..222	PF03717 Penicillin-binding Protein dimerisation domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ftsI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128077	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944799	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000309	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AD68	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10341	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10341	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944799	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000335	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0337	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	349	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	144	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	226	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Localization of FtsI depends on prior localization of FtsA and FtsZ. <ref_name='PMID:9603865'/>

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:91393..91433 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0084 (EcoliWiki Page)	NCBI GEO profiles for ftsI
microarray	GenExpDB:b0084 (EcoliWiki Page)	Summary of data for ftsI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ftsI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10341	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0337	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0084
EcoGene	EcoGene:EG10341	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000335	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000309	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FTSI	From SHIGELLACYC
E. coli O157	FTSI	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00905 Penicillin binding protein transpeptidase domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:56519
Superfamily (EcoliWiki Page)	SUPERFAMILY:56601
Pfam (EcoliWiki Page)	PF03717 Penicillin-binding Protein dimerisation domain
EcoCyc	EcoCyc:EG10341	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10341	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000335	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0337	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000309	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ftsI:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ftsI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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