folP:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

folP

Mnemonic

Folate

Synonyms

ECK3166, b3177, JW3144, dhpS[1], dhpS

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

71.6 minutes 

MG1655: 3322933..3322085
<gbrowseImage> name=NC_000913:3322085..3322933 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3260145..3259297
<gbrowseImage> name=NC_012967:3259297..3260145 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3209233..3210081
<gbrowseImage> name=NC_012759:3209233..3210081 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3324766..3323918
<gbrowseImage> name=NC_007779:3323918..3324766 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3420678..3419830
<gbrowseImage> name=NC_010473:3419830..3420678 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3322085

Edman degradation

PMID:1657875[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

folPF28I

F28I

(in TS20; resistant to sulfonamide and temperature-sensitive)

Strain variation; seeded from UniProt:P0AC13

ΔfolP (Keio:JW3144)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10389[4]

folP::Tn5KAN-I-SceI (FB21059)

Insertion at nt 838 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21059

contains pKD46

ΔfolP775::kan

PMID:16738554[3]

CGSC:100986


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Notes

The Keio collection[3] lists a deletion of folP. The insertion in this strain is a duplication of the folP region.[6]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3144

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAACTCTTTGCCCAGGGTAC

Primer 2:CCtTCATAGCGTTTGTTTTCCTT

18H7

Kohara Phage

Genobase

PMID:3038334[8]

14F11

Kohara Phage

Genobase

PMID:3038334[8]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[4]

est. P1 cotransduction: % [9]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[4]

est. P1 cotransduction: 73% [9]
Synonyms:zgj-203::Tn10, zha-203::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG50011

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG50011

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002570

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947691

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4304

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010441

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Talarico, TL et al. (1991) Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100. J. Bacteriol. 173 7029-32 PubMed
  3. 3.0 3.1 3.2 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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