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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	folE
Gene Synonym(s)	ECK2146, b2153, JW2140^[1], JW2140
Product Desc.	GTP cyclohydrolase I^[2]^[3]; Component of GTP cyclohydrolase I^[2]^[3] GTP cyclohydrolase I^[4]
Product Synonyms(s)	GTP cyclohydrolase I^[1], B2153^[2]^[1], FolE^[2]^[1] , ECK2146, JW2140, b2153
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yeiB^[2]
Regulation/Activity
Quick Links
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Notes

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Although a folE null mutant could not be made by Baba (2006), leading to its inclusion in the set of 303 essential genes, Klaus (2005) were able to construct a deletion mutation in folE. It requires LB+ thymidine and grows poorly, explaining the Baba negative result. Thus, folE is not an essential gene. FolE is the first enzyme of pre-Q(0) biosynthesis (Phillips, 2008).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	folE
Mnemonic	Folate
Synonyms	ECK2146, b2153, JW2140^[1], JW2140
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	48.3 minutes	MG1655: 2241674..2241006 <gbrowseImage> name=NC_000913:2241006..2241674 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2194890..2194222 <gbrowseImage> name=NC_012967:2194222..2194890 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2133489..2134157 <gbrowseImage> name=NC_012759:2133489..2134157 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2246986..2246318 <gbrowseImage> name=NC_007779:2246318..2246986 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2332663..2331995 <gbrowseImage> name=NC_010473:2331995..2332663 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2241009	Edman degradation	PMID:1459137 PMID:1665332 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2140	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCATCACTCAGTAAAGAAGC Primer 2:CCGTTGTGATGACGCACAGCGCG
7F1	Kohara Phage	Genobase	PMID:3038334
7H12	Kohara Phage	Genobase	PMID:3038334
5H11	Kohara Phage	Genobase	PMID:3038334
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: 3% ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
napA722::Tn10	Linked marker	CAG12098 = CGSC7400^[5]	est. P1 cotransduction: 4% ^[6] Synonyms:zeg-722, zei-722::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11375	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11375	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001343	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949040	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1349	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007117	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FolE
Synonyms	GTP cyclohydrolase I^[1], B2153^[2]^[1], FolE^[2]^[1] , ECK2146, JW2140, b2153
Product description	GTP cyclohydrolase I^[2]^[3]; Component of GTP cyclohydrolase I^[2]^[3] GTP cyclohydrolase I^[4]
EC number (for enzymes)	3.5.4.16^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	PMID:821948	IDA: Inferred from Direct Assay		C	FolE was present in the supernatant after subcellular fractionation. Seeded from Riley et al 2006 ^[1].	complete
	GO:0005737	cytoplasm	PMID:4296838	IDA: Inferred from Direct Assay		C	GTP cyclohyrdolase I activity was purified from the supernatant after subcellular fractionation.	complete
	GO:0003934	GTP cyclohydrolase I activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00223	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003934	GTP cyclohydrolase I activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001474	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005525	GTP binding	PMID:821948	IDA: Inferred from Direct Assay		F		complete
	GO:0003934	GTP cyclohydrolase I activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018234	F	Seeded from EcoCyc (v14.0)	complete
	GO:0032991	protein-containing complex	PMID:821948	IDA: Inferred from Direct Assay		C	Based on biochemical characterization.	complete
	GO:0003934	GTP cyclohydrolase I activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.5.4.16	F	Seeded from EcoCyc (v14.0)	complete
	GO:0032991	protein-containing complex	PMID:7663943	IDA: Inferred from Direct Assay		C	FolE is a homodecamer based on X-ray crystallography.	complete
	GO:0005525	GTP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0342	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006730	one-carbon metabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00223	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006730	one-carbon metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0554	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046654	tetrahydrofolate biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00223	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046654	tetrahydrofolate biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001474	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046654	tetrahydrofolate biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018234	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of GTP cyclohydrolase I	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPSLSKEAAL VHEALVARGL ETPLRPPVHE MDNETRKSLI AGHMTEIMQL LNLDLADDSL METPHRIAKM YVDEIFSGLD YANFPKITLI ENKMKVDEMV TVRDITLTST CEHHFVTIDG KATVAYIPKD SVIGLSKINR IVQFFAQRPQ VQERLTQQIL IALQTLLGTN NVAVSIDAVH YCVKARGIRD ATSATTTTSL GGLFKSSQNT RHEFLRAVRH HN
Length	222
Mol. Wt	24.83 kDa
pI	7.4 (calculated)
Extinction coefficient	5,960 - 6,210 (calc based on 4 Y, 0 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A6T5
Domain	117..202	PF01227 GTP cyclohydrolase I		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=folE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130091	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949040	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007117	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6T5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11375	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11375	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949040	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001343	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1349	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	7.37E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	4658	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	9024	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	8104	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yeiB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2241654..2241694 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2153 (EcoliWiki Page)	NCBI GEO profiles for folE
microarray	GenExpDB:b2153 (EcoliWiki Page)	Summary of data for folE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2241600..2241941) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:H2^[7]
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Notes

Accessions Related to folE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11375	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1349	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2153
EcoGene	EcoGene:EG11375	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001343	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007117	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000013527 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000017411 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G07270 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000009020 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00031200 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00000298 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000022043 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000008998 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-001205-3 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040625-159 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0191399 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0003162 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA21789-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000019895 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000254299 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000005461 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000014636 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:95675 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000010797 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000014821 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YGR267C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC17A53 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000141488 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000164083 (score: 0.546) NEWSINFRUP00000157715 (score: 0.222)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00034788001 (score: 1.000; bootstrap: 100%) GSTENP00005535001 (score: 0.544) GSTENP00019319001 (score: 0.221)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000026690 (score: 1.000; bootstrap: 72%) ENSXETP00000049200 (score: 0.536)	From Inparanoid:20070104
Shigella flexneri	FOLE	From SHIGELLACYC
E. coli O157	FOLE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01227 GTP cyclohydrolase I
Panther (EcoliWiki Page)	PTHR11109:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:55620
EcoCyc	EcoCyc:EG11375	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11375	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001343	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1349	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007117	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

folE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to folE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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