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On One Page

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Expression|

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Standard Name	flgM
Gene Synonym(s)	ECK1056, b1071, JW1058^[1], JW1058, mviS
Product Desc.	anti-sigma factor for FliA (sigma 28)^[2]^[3] Anti-sigma 28 (FliA) factor; regulator of FlhD^[4]
Product Synonyms(s)	anti-sigma factor for FliA (sigma 28)^[1], B1071^[2]^[1], FlgM^[2]^[1] , ECK1056, JW1058, mviS, b1071
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): flgMN^[2], flgAMN^[2]
Regulation/Activity
Quick Links
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Notes

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Flk prevents premature FlgM secretion. FlgM function is stopped when it is secreted into the periplasm or the medum. FlgM is characterized primarily in Salmonella; the fMet of Salmonella FlgM is clipped. yceN was identified as one of two ORFs adjacent to the flg locus in a region originally named mviS, then renamed mviN and mviM when sequenced; mviMN was thought to be required for mouse virulence in Salmonella, but the mviMN deletion in ST39 also removed flgM (Carsiotis, 1989). flgM was later shown to be able to complement the mviS phenotype, and thus flgM is the real mouse virulence gene mviS in this region of the Salmonella chromosome (Schmitt, 1994).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	flgM
Mnemonic	Flagella
Synonyms	ECK1056, b1071, JW1058^[1], JW1058, mviS
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	24.33 minutes	MG1655: 1129351..1129058 <gbrowseImage> name=NC_000913:1129058..1129351 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1144700..1144407 <gbrowseImage> name=NC_012967:1144407..1144700 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1032983..1033276 <gbrowseImage> name=NC_012759:1032983..1033276 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1131705..1131412 <gbrowseImage> name=NC_007779:1131412..1131705 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1184796..1184503 <gbrowseImage> name=NC_010473:1184503..1184796 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔflgM (Keio:JW1058)	deletion	deletion	PMID:16738554	Shigen CGSC9015^[5]
flgM::Tn5KAN-2 (FB20094)	Insertion at nt 67 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20094	does not contain pKD46
ΔflgM740::kan			PMID:16738554	CGSC:100920
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1058	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTATTGATCGCACTTCGCC Primer 2:CCGTTACTCTGCAAGTCTTGCTG
E4H10S	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: 2% ^[6]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 37% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G369	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12664	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002689	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946684	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2530	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003634	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FlgM
Synonyms	anti-sigma factor for FliA (sigma 28)^[1], B1071^[2]^[1], FlgM^[2]^[1] , ECK1056, JW1058, mviS, b1071
Product description	anti-sigma factor for FliA (sigma 28)^[2]^[3] Anti-sigma 28 (FliA) factor; regulator of FlhD^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016481	negative regulation of transcription	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007412	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016564	transcription repressor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007412	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019861	flagellum	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007412	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	fliA	PMID:16606699	Experiment(s):EBI-1138760
Protein	gltX	PMID:16606699	Experiment(s):EBI-1138760
Protein	yahG	PMID:16606699	Experiment(s):EBI-1138760
Protein	tatE	PMID:16606699	Experiment(s):EBI-1138760
Protein	pepD	PMID:16606699	Experiment(s):EBI-1138760
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1138760
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSIDRTSPLK PVSTVQPRET TDAPVTNSRA AKTTASTSTS VTLSDAQAKL MQPGSSDINL ERVEALKLAI RNGELKMDTG KIADALINEA QQDLQSN
Length	97
Mol. Wt	10.34 kDa
pI	5.2 (calculated)
Extinction coefficient	0 (calc based on 0 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	25..90	PF04316 Anti-sigma-28 factor, FlgM		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=flgM taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

FlgM is an intrinsically disordered protein in vitro. In vivo, there is evidence that it gains structure^[7].

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129034	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946684	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003634	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEM4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G369	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12664	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946684	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002689	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2530	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	136	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	151	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	85^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
flgMN
flgAMN
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1129331..1129371 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1071 (EcoliWiki Page)	NCBI GEO profiles for flgM
microarray	GenExpDB:b1071 (EcoliWiki Page)	Summary of data for flgM from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1129264..1129463) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:B2^[8]
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Notes

Accessions Related to flgM Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G369	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2530	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1071
EcoGene	EcoGene:EG12664	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002689	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003634	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FLGM	From SHIGELLACYC
E. coli O157	FLGM	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04316 Anti-sigma-28 factor, FlgM
Superfamily (EcoliWiki Page)	SUPERFAMILY:101498
EcoCyc	EcoCyc:G369	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12664	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002689	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2530	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003634	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Dedmon, MM et al. (2002) FlgM gains structure in living cells. Proc. Natl. Acad. Sci. U.S.A. 99 12681-4 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

flgM:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to flgM Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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