flgI:On One Page

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

<protect>

Standard Name

flgI

Gene Synonym(s)

ECK1065, b1080, JW1067, flaM, fla FIX, fla, F, FIX[1][2]

Product Desc.

flagellar P-ring protein FlgI[2][3];

Component of Flagellar Motor Complex[2][3]; Flagellum[3]

Product Synonyms(s)

predicted flagellar basal body protein[1], B1080[2][1], Fla[2][1], FlaM[2][1], FlgI[2][1] , ECK1065, flaM, JW1067, b1080

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): flgBCDEFGHIJ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]



Gene

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

flgI

Mnemonic

Flagella

Synonyms

ECK1065, b1080, JW1067, flaM, fla FIX, fla, F, FIX[1][2]

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

24.47 minutes 

MG1655: 1135497..1136594
<gbrowseImage> name=NC_000913:1135497..1136594 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1150843..1151940
<gbrowseImage> name=NC_012967:1150843..1151940 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1039422..1040519
<gbrowseImage> name=NC_012759:1039422..1040519 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1137851..1138948
<gbrowseImage> name=NC_007779:1137851..1138948 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1190942..1192039
<gbrowseImage> name=NC_010473:1190942..1192039 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔflgI (Keio:JW1067)

deletion

deletion

PMID:16738554

Shigen
CGSC9023[4]

ΔflgI749::kan

PMID:16738554

CGSC:100914


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1067

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATTAAATTTCTCTCTGCATT

Primer 2:CCGATGATTTCCAGTTTTGCCCG

E3G11

Kohara Phage

Genobase

PMID:3038334

14C1

Kohara Phage

Genobase

PMID:3038334

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[4]

est. P1 cotransduction: 1% [5]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[4]

est. P1 cotransduction: 49% [5]

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G365

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20265

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002687

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947534

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4162

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003656

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



Product(s)

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FlgI

Synonyms

predicted flagellar basal body protein[1], B1080[2][1], Fla[2][1], FlaM[2][1], FlgI[2][1] , ECK1065, flaM, JW1067, b1080

Product description

flagellar P-ring protein FlgI[2][3];

Component of Flagellar Motor Complex[2][3]; Flagellum[3]

EC number (for enzymes)


<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0001539

ciliary or flagellar motility

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00416

P

Seeded from EcoCyc (v14.0)

complete

GO:0001539

ciliary or flagellar motility

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001782

P

Seeded from EcoCyc (v14.0)

complete

GO:0005198

structural molecule activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001782

F

Seeded from EcoCyc (v14.0)

complete

GO:0009288

bacterial-type flagellum

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0975

C

Seeded from EcoCyc (v14.0)

complete

GO:0009425

bacterial-type flagellum basal body

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0142

C

Seeded from EcoCyc (v14.0)

complete

GO:0009428

bacterial-type flagellum basal body, distal rod, P ring

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001782

C

Seeded from EcoCyc (v14.0)

complete

GO:0030288

outer membrane-bounded periplasmic space

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001782

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0200

C

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Flagellar Motor Complex

could be indirect

Protein

Subunits of Flagellum

could be indirect


</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Periplasm

PMID:12730325

EchoLocation:flgI


<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MIKFLSALIL LLVTTAAQAE RIRDLTSVQG VRQNSLIGYG LVVGLDGTGD QTTQTPFTTQ
TLNNMLSQLG ITVPTGTNMQ LKNVAAVMVT ASLPPFGRQG QTIDVVVSSM GNAKSLRGGT
LLMTPLKGVD SQVYALAQGN ILVGGAGASA GGSSVQVNQL NGGRITNGAV IERELPSQFG
VGNTLNLQLN DEDFSMAQQI ADTINRVRGY GSATALDART IQVRVPSGNS SQVRFLADIQ
NMQVNVTPQD AKVVINSRTG SVVMNREVTL DSCAVAQGNL SVTVNRQANV SQPDTPFGGG
QTVVTPQTQI DLRQSGGSLQ SVRSSASLNN VVRALNALGA TPMDLMSILQ SMQSAGCLRA
KLEII
Length

365

Mol. Wt

38.168 kDa

pI

9.6 (calculated)

Extinction coefficient

4,470 - 4,720 (calc based on 3 Y, 0 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-19

UniProt Manual:Signal Peptides

UniProt:P0A6S3

Domain

21..365

PF02119 Flagellar P-ring protein

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=flgI taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176076

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947534

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003656

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6S3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G365

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20265

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947534

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002687

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4162

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]




Expression

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

13

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

8

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

[back to top]


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

flgBCDEFGHIJ

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


<protect><gbrowseImage> name=NC_000913:1135477..1135517 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1080 (EcoliWiki Page)

NCBI GEO profiles for flgI

microarray

GenExpDB:b1080 (EcoliWiki Page)

Summary of data for flgI from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

<protect></protect>

Notes

Accessions Related to flgI Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G365

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4162

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1080

EcoGene

EcoGene:EG20265

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002687

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003656

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



Evolution

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

FLGI

From SHIGELLACYC

E. coli O157

FLGI

From ECOO157CYC


Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02119 Flagellar P-ring protein

EcoCyc

EcoCyc:G365

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20265

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002687

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4162

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003656

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

[back to top]