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Expression|

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Standard Name	fhuB
Gene Synonym(s)	ECK0152, b0153, JW0149^[1], JW0149
Product Desc.	FhuB^[2]^[3]; Component of iron (III) hydroxamate ABC transporter^[2]^[3]; ferrichrome uptake system^[3] Ferrichrome-dependent iron uptake, ABC transporter permease; integral cytoplasmic membrane component^[4]
Product Synonyms(s)	fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components^[1], B0153^[2]^[1], FhuB^[2]^[1] , ECK0152, JW0149, b0153
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fhuACDB^[2], OP00027, fhuA
Regulation/Activity
Quick Links
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Notes

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fhuB mutants are albomycin resistant. FhuB has 20 transmembrane helices. The N- and C-terminal halves of FhuB are homologous.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fhuB
Mnemonic	Ferric hydroxamate uptake
Synonyms	ECK0152, b0153, JW0149^[1], JW0149
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	3.7 minutes	MG1655: 171462..173444 <gbrowseImage> name=NC_000913:171462..173444 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 174304..176286 <gbrowseImage> name=NC_012967:174304..176286 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 171461..173443 <gbrowseImage> name=NC_012759:171461..173443 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 171462..173444 <gbrowseImage> name=NC_007779:171462..173444 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 145566..147548 <gbrowseImage> name=NC_010473:145566..147548 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
fhuB(del) (Keio:JW0149)	deletion	deletion	PMID:16738554	Shigen CGSC8419^[5]
fhuB::Tn5KAN-2 (FB20024)	Insertion at nt 180 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20024	does not contain pKD46
fhuB478::Tn10				CGSC:8770
fhuB769(del)::kan			PMID:16738554	CGSC:100850
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0149	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTAAACGAATTGCGCTTTT Primer 2:CCACGGCTCTGCTTTCTCAACAA
15A7	Kohara Phage	Genobase	PMID:3038334
9H2	Kohara Phage	Genobase	PMID:3038334
4E4	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 43% ^[6]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 4% ^[6] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10303	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10303	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000297	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946890	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0299	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000526	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FhuB
Synonyms	fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components^[1], B0153^[2]^[1], FhuB^[2]^[1] , ECK0152, JW0149, b0153
Product description	FhuB^[2]^[3]; Component of iron (III) hydroxamate ABC transporter^[2]^[3]; ferrichrome uptake system^[3] Ferrichrome-dependent iron uptake, ABC transporter permease; integral cytoplasmic membrane component^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000522	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000522	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006811	ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006826	iron ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0410	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000522	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of iron (III) hydroxamate ABC transporter	could be indirect
Protein	Subunits of ferrichrome uptake system	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 16 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSKRIALFPA LLLALLVIVA TALTWMNFSQ ALPRSQWAQA AWSPDIDVIE QMIFHYSLLP RLAISLLVGA GLGLVGVLFQ QVLRNPLAEP TTLGVATGAQ LGITVTTLWA IPGAMASQFA AQAGACVVGL IVFGVAWGKR LSPVTLILAG LVVSLYCGAI NQLLVIFHHD QLQSMFLWST GTLTQTDWGG VERLWPQLLG GVMLTLLLLR PLTLMGLDDG VARNLGLALS LARLAALSLA IVISALLVNA VGIIGFIGLF APLLAKMLGA RRLLPRLMLA SLIGALILWL SDQIILWLTR VWMEVSTGSV TALIGAPLLL WLLPRLRSIS APDMKVNDRV AAERQHVLAF ALAGGVLLLM AVVVALSFGR DAHGWTWASG ALLEDLMPWR WPRIMAALFA GVMLAVAGCI IQRLTGNPMA SPEVLGISSG AAFGVVLMLF LVPGNAFGWL LPAGSLGAAV TLLIIMIAAG RGGFSPHRML LAGMALSTAF TMLLMMLQAS GDPRMAQVLT WISGSTYNAT DAQVWRTGIV MVILLAITPL CRRWLTILPL GGDTARAVGM ALTPTRIALL LLAACLTATA TMTIGPLSFV GLMAPHIARM MGFRRTMPHI VISALVGGLL LVFADWCGRM VLFPFQIPAG LLSTFIGAPY FIYLLRKQSR
Length	660
Mol. Wt	70.426 kDa
pI	11.3 (calculated)
Extinction coefficient	122,950 - 123,700 (calc based on 5 Y, 21 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	7..325	PF01032 FecCD transport family	PMID:19920124
Domain	347..657	PF01032 FecCD transport family	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fhuB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128146	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946890	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000526	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P06972	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10303	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10303	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946890	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000297	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0299	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	68	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	18^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fhuACDB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:171442..171482 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0153 (EcoliWiki Page)	NCBI GEO profiles for fhuB
microarray	GenExpDB:b0153 (EcoliWiki Page)	Summary of data for fhuB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to fhuB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10303	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0299	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0153
EcoGene	EcoGene:EG10303	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000297	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000526	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FHUB	From SHIGELLACYC
E. coli O157	FHUB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01032 FecCD transport family
Pfam (EcoliWiki Page)	PF01032 FecCD transport family
Superfamily (EcoliWiki Page)	SUPERFAMILY:81345
Superfamily (EcoliWiki Page)	SUPERFAMILY:81345
EcoCyc	EcoCyc:EG10303	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10303	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000297	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0299	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000526	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

fhuB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fhuB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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