fhuA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fhuA

Mnemonic

Ferric hydroxamate uptake

Synonyms

ECK0149, b0150, JW0146, T1, T5rec, tonA[1], tonA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

3.61 minutes 

MG1655: 167484..169727
<gbrowseImage> name=NC_000913:167484..169727 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 170326..172569
<gbrowseImage> name=NC_012967:170326..172569 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 167483..169726
<gbrowseImage> name=NC_012759:167483..169726 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 167484..169727
<gbrowseImage> name=NC_007779:167484..169727 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 141588..143831
<gbrowseImage> name=NC_010473:141588..143831 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

167583

Edman degradation

PMID:3079747[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

W254X

Nonsynonomous mutation

PMID:18245285[3]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

fhuA(del) (Keio:JW0146)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC8416[5]

fhuA(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[6]

fhuA23

CGSC:5378

fhuA2

CGSC:6386

fhuA21

CGSC:6399

fhuA54

CGSC:6622

fhuA27

CGSC:6662

fhuA67

CGSC:7544

fhuA48

CGSC:8557

fhuA49

CGSC:8560

fhuA1

CGSC:9063

fhuA22

CGSC:9545

fhuA24

CGSC:9546

fhuA14

CGSC:9612

fhuA31

CGSC:9684

fhuA61

CGSC:9826

fhuA16

CGSC:10254

fhuA53

CGSC:10282

fhuA13

CGSC:10327

fhuA25

CGSC:11067

fhuA66

CGSC:11117

fhuA32

CGSC:11550

fhuA58

CGSC:12957

fhuA46

CGSC:14297

fhuA57

CGSC:14331

fhuA47

CGSC:14447

fhuA55

CGSC:14558

fhuA50

CGSC:16324

fhuA51

CGSC:16325

fhuA52

CGSC:16326

fhuA12

CGSC:16905

fhuA26

CGSC:16922

fhuA0

CGSC:75345

fhuA766(del)::kan

PMID:16738554[4]

CGSC:100848

fhuA(del)

Resistant to

Resistant to phage T2.

PMID:1444296[7]

Experimental Strain:EKDr

see figure 5 & 9

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0146

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCGCGCGTTCCAAAACTGCTCA

Primer 2:CCGAAACGGAAGGTTGCGGTTGC

15A7

Kohara Phage

Genobase

PMID:3038334[9]

9H2

Kohara Phage

Genobase

PMID:3038334[9]

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 50% [10]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 3% [10]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10302

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10302

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000296

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944856

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0298

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000520

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Coulton, JW et al. (1986) Protein fusions of beta-galactosidase to the ferrichrome-iron receptor of Escherichia coli K-12. J. Bacteriol. 165 181-92 PubMed
  3. Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. Brochu, A et al. (1992) Modes of action and inhibitory activities of new siderophore-beta-lactam conjugates that use specific iron uptake pathways for entry into bacteria. Antimicrob. Agents Chemother. 36 2166-75 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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