{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	fbaA
Gene Synonym(s)	ECK2921, b2925, JW2892, ald, fda, fba^[1], fba
Product Desc.	fructose bisphosphate aldolase^[2]^[3]; Component of fructose bisphosphate aldolase class II^[2]^[3] Fructose-bisphosphate aldolase, class II; binds Zn(II); homdomeric^[4]
Product Synonyms(s)	fructose-bisphosphate aldolase, class II^[1], B2925^[2]^[1], Fda^[2]^[1], Ald^[2]^[1], FbaA^[2]^[1], Fba^[2]^[1] , ald, ECK2921, fba, fda, JW2892, b2925
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): epd-pgk-fbaA^[2], pgk-fbaA^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Binds TrxA (Kumar, 2004). kbaY, gatY, fbaA and ydjI are paralogs.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fbaA
Mnemonic	Fructose bis-P aldolase
Synonyms	ECK2921, b2925, JW2892, ald, fda, fba^[1], fba
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	66.13 minutes	MG1655: 3069266..3068187 <gbrowseImage> name=NC_000913:3068187..3069266 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2956995..2955916 <gbrowseImage> name=NC_012967:2955916..2956995 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2955335..2956414 <gbrowseImage> name=NC_012759:2955335..2956414 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3069900..3068821 <gbrowseImage> name=NC_007779:3068821..3069900 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3163136..3162057 <gbrowseImage> name=NC_010473:3162057..3163136 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	AA change(s)	Phenotype: Description	Reference	Availability	Comments
fbaAN36A	N36A	1.5% of wild-type activity. 5-fold decrease in FBP affinity			seeded from UniProt:P0AB71
fbaAQ60A	Q60A	81% of wild-type activity. 1.3-fold decrease in FBP affinity			seeded from UniProt:P0AB71
fbaAK326A	K326A	6% of wild-type activity. 2.2-fold decrease in FBP affinity			seeded from UniProt:P0AB71
fbaAC112A	C112A	Partial loss of activity			seeded from UniProt:P0AB71
fbaAS62A	S62A	8% of wild-type activity. 16-fold decrease in FBP affinity			seeded from UniProt:P0AB71
fbaAS62T	S62T	60% of wild-type activity. 2.5-fold decrease in FBP affinity			seeded from UniProt:P0AB71
fbaAH108A	H108A	Loss of activity			seeded from UniProt:P0AB71
fbaAH111A	H111A	Loss of activity			seeded from UniProt:P0AB71
fbaA2(ts)		temperature sensitive	PMID:16562136 PMID:1717436	CGSC:6161
fbaA1				CGSC:6134
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2892	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTAAGATTTTTGATTTCGT Primer 2:CCaAGAACGTCGATCGCGTTCAG
6C5	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: % ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: 54% ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10282	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10282	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000276	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947415	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0278	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009600	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FbaA
Synonyms	fructose-bisphosphate aldolase, class II^[1], B2925^[2]^[1], Fda^[2]^[1], Ald^[2]^[1], FbaA^[2]^[1], Fba^[2]^[1] , ald, ECK2921, fba, fda, JW2892, b2925
Product description	fructose bisphosphate aldolase^[2]^[3]; Component of fructose bisphosphate aldolase class II^[2]^[3] Fructose-bisphosphate aldolase, class II; binds Zn(II); homdomeric^[4]
EC number (for enzymes)	4.1.2.13^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004332	fructose-bisphosphate aldolase activity	PMID:16562136	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0006094	gluconeogenesis				P		Missing: evidence, reference
	GO:0004332	fructose-bisphosphate aldolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000771	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004332	fructose-bisphosphate aldolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006411	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004332	fructose-bisphosphate aldolase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.2.13	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000771	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006411	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0324	P	Seeded from EcoCyc (v14.0)	complete
	GO:0004332	fructose-bisphosphate aldolase activity	PMID:16562136	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	PMID:11985624	IDA: Inferred from Direct Assay		F		complete
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000771	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004332	fructose-bisphosphate aldolase activity	PMID: 16562136	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	PMID:8436219	IDA: Inferred from Direct Assay		F		complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of fructose bisphosphate aldolase class II	could be indirect
Protein	dnaK		PMID:15690043	Experiment(s):EBI-879773, EBI-884401
Protein	fldA		PMID:15690043	Experiment(s):EBI-879773
Protein	hsdR		PMID:15690043	Experiment(s):EBI-879773
Protein	rpsB		PMID:15690043	Experiment(s):EBI-879773
Protein	yfeR		PMID:15690043	Experiment(s):EBI-879773
Protein	fldA		PMID:19402753	MALDI(Z-score):17.053160
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Cytoplasm		PMID:2649077	EchoLocation:fbaA
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSKIFDFVKP GVITGDDVQK VFQVAKENNF ALPAVNCVGT DSINAVLETA AKVKAPVIVQ FSNGGASFIA GKGVKSDVPQ GAAILGAISG AHHVHQMAEH YGVPVILHTD HCAKKLLPWI DGLLDAGEKH FAATGKPLFS SHMIDLSEES LQENIEICSK YLERMSKIGM TLEIELGCTG GEEDGVDNSH MDASALYTQP EDVDYAYTEL SKISPRFTIA ASFGNVHGVY KPGNVVLTPT ILRDSQEYVS KKHNLPHNSL NFVFHGGSGS TAQEIKDSVS YGVVKMNIDT DTQWATWEGV LNYYKANEAY LQGQLGNPKG EDQPNKKYYD PRVWLRAGQT SMIARLEKAF QELNAIDVL
Length	359
Mol. Wt	39.146 kDa
pI	5.8 (calculated)
Extinction coefficient	41,370 - 41,870 (calc based on 13 Y, 4 W, and 4 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0AB71
Domain	14..359	PF01116 Fructose-bisphosphate aldolase class-II		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fbaA taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130826	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947415	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009600	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AB71	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10282	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10282	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947415	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000276	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0278	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	4.78E+04	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	676.369+/-4.653	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.21362+/-0.02498	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
Protein	E. coli K-12 MG1655	26609	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	13978	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	15477	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
epd-pgk-fbaA
pgk-fbaA
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:3069246..3069286 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2925 (EcoliWiki Page)	NCBI GEO profiles for fbaA
microarray	GenExpDB:b2925 (EcoliWiki Page)	Summary of data for fbaA from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to fbaA Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10282	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0278	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2925
EcoGene	EcoGene:EG10282	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000276	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009600	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Saccharomyces cerevisiae	YKL060C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC19C27 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	FBA	From SHIGELLACYC
E. coli O157	FBA	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01116 Fructose-bisphosphate aldolase class-II
Superfamily (EcoliWiki Page)	SUPERFAMILY:51569
EcoCyc	EcoCyc:EG10282	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10282	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000276	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0278	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009600	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

fbaA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fbaA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search