fabH:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

fabH

Gene Synonym(s)

ECK1077, b1091, JW1077[1], JW1077

Product Desc.

FABH[2][3];

Component of β-ketoacyl-acyl carrier protein synthase III[3]

beta-Ketoacyl-ACP synthase III; KAS III; monomer[4]

Product Synonyms(s)

3-oxoacyl-[acyl-carrier-protein] synthase III[1], B1091[2][1], FabH[2][1] , ECK1077, JW1077, b1091

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): plsX-fabHDG[2], rpmF-plsX-fabHDG[2], fabHDG[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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fabH is an essential gene.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fabH

Mnemonic

Fatty acid biosynthesis

Synonyms

ECK1077, b1091, JW1077[1], JW1077

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

24.74 minutes 

MG1655: 1147982..1148935
<gbrowseImage> name=NC_000913:1147982..1148935 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1163328..1164281
<gbrowseImage> name=NC_012967:1163328..1164281 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1051907..1052860
<gbrowseImage> name=NC_012759:1051907..1052860 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1150336..1151289
<gbrowseImage> name=NC_007779:1150336..1151289 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1203427..1204380
<gbrowseImage> name=NC_010473:1203427..1204380 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1147982

Edman degradation

PMID:1551888


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔfabH (Keio:JW1077)

deletion

deletion

PMID:16738554

Shigen
CGSC8298[5]

fabHKK256EE

KK256EE

Abolishes the binding to malonyl- ACP but not that of the substrate

seeded from UniProt:P0A6R0

fabHN274A

N274A

Loss of activity

seeded from UniProt:P0A6R0

fabHA253Y

A253Y

Abolishes both binding to malonyl- ACP and binding to substrate

seeded from UniProt:P0A6R0

fabHKK256AA

KK256AA

Strongly reduces both binding to malonyl-ACP and binding to substrate

seeded from UniProt:P0A6R0

fabHC112S

C112S

Loss of activity

seeded from UniProt:P0A6R0

fabHK214E,A

K214E,A

Strongly reduces the binding to malonyl-ACP but not that of the substrate

seeded from UniProt:P0A6R0

fabHH244A

H244A

Loss of activity

seeded from UniProt:P0A6R0

fabHR249E,A

R249E,A

Abolishes the binding to malonyl- ACP but not that of the substrate

seeded from UniProt:P0A6R0

ΔfabH721::kan

PMID:16738554

CGSC:99702


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1077

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTATACGAAGATTATTGGTAC

Primer 2:CCGAAACGAACCAGCGCGGAGCC

14C1

Kohara Phage

Genobase

PMID:3038334

E9G1

Kohara Phage

Genobase

PMID:3038334

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[5]

est. P1 cotransduction: % [6]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[5]

est. P1 cotransduction: 78% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10277

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10277

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000271

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946003

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0273

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003696

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FabH

Synonyms

3-oxoacyl-[acyl-carrier-protein] synthase III[1], B1091[2][1], FabH[2][1] , ECK1077, JW1077, b1091

Product description

FABH[2][3];

Component of β-ketoacyl-acyl carrier protein synthase III[3]

beta-Ketoacyl-ACP synthase III; KAS III; monomer[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016038

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016039

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0511

F

Seeded from EcoCyc (v14.0)

complete

GO:0004315

3-oxoacyl-(acyl-carrier-protein) synthase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01815

F

Seeded from EcoCyc (v14.0)

complete

GO:0004315

3-oxoacyl-(acyl-carrier-protein) synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004655

F

Seeded from EcoCyc (v14.0)

complete

GO:0004315

3-oxoacyl-(acyl-carrier-protein) synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013751

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01815

P

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004655

P

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013751

P

Seeded from EcoCyc (v14.0)

complete

GO:0006633

fatty acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0275

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016038

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016039

P

Seeded from EcoCyc (v14.0)

complete

GO:0008415

acyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0012

F

Seeded from EcoCyc (v14.0)

complete

GO:0008610

lipid biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013747

P

Seeded from EcoCyc (v14.0)

complete

GO:0008610

lipid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0444

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016747

transferase activity, transferring acyl groups other than amino-acyl groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013747

F

Seeded from EcoCyc (v14.0)

complete

GO:0033818

beta-ketoacyl-acyl-carrier-protein synthase III activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.3.1.180

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of β-ketoacyl-acyl carrier protein synthase III

could be indirect

Protein

nadE

PMID:16606699

Experiment(s):EBI-1138826

Protein

cca

PMID:16606699

Experiment(s):EBI-1138826

Protein

intS

PMID:16606699

Experiment(s):EBI-1138826
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MYTKIIGTGS YLPEQVRTNA DLEKMVDTSD EWIVTRTGIR ERHIAAPNET VSTMGFEAAT
RAIEMAGIEK DQIGLIVVAT TSATHAFPSA ACQIQSMLGI KGCPAFDVAA ACAGFTYALS
VADQYVKSGA VKYALVVGSD VLARTCDPTD RGTIIIFGDG AGAAVLAASE EPGIISTHLH
ADGSYGELLT LPNADRVNPE NSIHLTMAGN EVFKVAVTEL AHIVDETLAA NNLDRSQLDW
LVPHQANLRI ISATAKKLGM SMDNVVVTLD RHGNTSAASV PCALDEAVRD GRIKPGQLVL
LEAFGGGFTW GSALVRF
Length

317

Mol. Wt

33.514 kDa

pI

5.0 (calculated)

Extinction coefficient

25,440 - 26,065 (calc based on 6 Y, 3 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

228..317

PF08541 3-Oxoacyl-[acyl-carrier-protein (ACP) synthase III C terminal ]

PMID:19920124

Domain

106..183

PF08545 3-Oxoacyl-[acyl-carrier-protein (ACP) synthase III]

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fabH taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129054

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946003

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003696

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6R0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10277

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10277

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946003

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000271

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0273

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.32E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

84.754+/-0.734

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.240818468

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

7355

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1564

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4455

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

plsX-fabHDG

rpmF-plsX-fabHDG

fabHDG

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1147962..1148002 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1091 (EcoliWiki Page)

NCBI GEO profiles for fabH

microarray

GenExpDB:b1091 (EcoliWiki Page)

Summary of data for fabH from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to fabH Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10277

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0273

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1091

EcoGene

EcoGene:EG10277

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000271

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003696

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT1G62640 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q7X8V9 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000023228 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

FABH

From SHIGELLACYC

E. coli O157

FABH

From ECOO157CYC

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

Pfam (EcoliWiki Page)

PF08541 3-Oxoacyl-[acyl-carrier-protein (ACP) synthase III C terminal ]

Pfam (EcoliWiki Page)

PF08545 3-Oxoacyl-[acyl-carrier-protein (ACP) synthase III]


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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