fabA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fabA

Mnemonic

Fatty acid biosynthesis

Synonyms

ECK0945, b0954, JW0937[1], JW0937

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

21.88 minutes 

MG1655: 1015693..1015175
<gbrowseImage> name=NC_000913:1015175..1015693 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1033561..1033043
<gbrowseImage> name=NC_012967:1033043..1033561 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 918143..918661
<gbrowseImage> name=NC_012759:918143..918661 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1016892..1016374
<gbrowseImage> name=NC_007779:1016374..1016892 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1069621..1069103
<gbrowseImage> name=NC_010473:1069103..1069621 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1015175

Edman degradation

PMID:2832401[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

fabAP76L

P76L

(in allele FABA6; TS)

Strain variation; seeded from UniProt:P0A6Q3

fabAG102D

G102D

(in allele FABA2; TS)

Strain variation; seeded from UniProt:P0A6Q3

fabA18(ts)

temperature sensitive

CGSC:6300

fabA2(ts)

temperature sensitive

CGSC:5236

fabA203(p)

PMID:6362720[3]

CGSC:7453


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0937

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGTAGATAAACGCGAATCCTA

Primer 2:CCGAAGGCAGACGTATCCTGGAA

E2E5

Kohara Phage

Genobase

PMID:3038334[5]

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[6]

est. P1 cotransduction: 64% [7]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[6]

est. P1 cotransduction: 12% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10273

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10273

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000267

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945568

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0269

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003229

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Cronan, JE Jr et al. (1988) Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase. J. Biol. Chem. 263 4641-6 PubMed
  3. Clark, DP et al. (1983) Beta-hydroxydecanoyl thio ester dehydrase does not catalyze a rate-limiting step in Escherichia coli unsaturated fatty acid synthesis. Biochemistry 22 5897-902 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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