emrE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
emrE |
|---|---|
| Mnemonic |
E.coli (or ethidium) multidrug resistance Efflux-multidrug resistance |
| Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
12.23 minutes |
MG1655: 567538..567870 |
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NC_012967: 540205..540537 |
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NC_012759: 470298..470630 |
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W3110 |
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W3110: 567538..567870 |
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DH10B: 506870..507202 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔemrE (Keio:JW0531) |
deletion |
deletion |
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|
emrEE14C |
E14C |
No substrate binding. No transport activity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEY4C |
Y4C |
Still binds substrate. No transport activity in the presence of a proton gradient, but still transports substrate in the absence of a proton gradient. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEW63C,Y |
W63C,Y |
No transport activity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
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emrEW63F |
W63F |
Still binds substrate, with two- fold reduction in substrate affinity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEY6C,F,L |
Y6C,F,L |
No effect on resistance to toxicants |
seeded from UniProt:P23895 | ||||
|
emrEY60C,F |
Y60C,F |
Still binds substrate, with lower affinity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEL7C |
L7C |
No substrate binding. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEE14D |
E14D |
Still binds substrate. No transport activity in the presence of a proton gradient, but still transports substrate in the absence of a proton gradient. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEY53C |
Y53C |
No effect on resistance to toxicants |
seeded from UniProt:P23895 | ||||
|
emrEI11C |
I11C |
Still binds substrate, with lower affinity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEA10C |
A10C |
Still binds substrate, with lower affinity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
|
emrEG17C |
G17C |
No substrate binding. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
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emrEY4F,W |
Y4F,W |
No effect on resistance to toxicants |
seeded from UniProt:P23895 | ||||
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emrET18C |
T18C |
Still binds substrate, with lower affinity. Resistance to toxicants is abolished |
seeded from UniProt:P23895 | ||||
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emrEY40C,F,L,M,S,T,V |
Y40C,F,L,M,S,T,V |
Modifies substrate specificity |
seeded from UniProt:P23895 | ||||
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ΔemrE750::kan |
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mrvC in strain MC48 |
Resistant to |
Resistance to Methyl Viologen |
Strain MC48 |
Figure 2 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0531 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAACCCTTATATTTATCTTGG Primer 2:CCATGTGGTGTGCTTCGTGACAA | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 58% [8] | ||
|
Linked marker |
est. P1 cotransduction: 2% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Morimyo, M et al. (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity. Nucleic Acids Res. 20 3159-65 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
