emrE:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

EmrE

Synonyms

DLP12 prophage; multidrug resistance protein[1], B0543[2][1], MvrC[2][1], EmrE[2][1] , eb, ECK0534, JW0531, mvrC, b0543

Product description

EmrE SMR transporter[2][3]

Multidrug resistance pump, DLP12 prophage; methylviologen sensitivity[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006805

xenobiotic metabolic process

PMID:1320256[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:12356478[6]

IDA: Inferred from Direct Assay

C

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

PMID:12356478[6]

IDA: Inferred from Direct Assay

P

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0042493

response to drug

PMID:7896833[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0015297

antiporter activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0050

F

Seeded from EcoCyc (v14.0)

complete

GO:0015297

antiporter activity

PMID:7896833[7]

IDA: Inferred from Direct Assay

F

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000390

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

PMID:9688273[8]

IDA: Inferred from Direct Assay

C

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071[9]

IEP: Inferred from Expression Pattern

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpmB

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

rpsT

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

rplK

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

fimA

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753[10]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized to the periplasm with 4 predicted transmembrane domains

Daley et al. (2005) [11]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MNPYIYLGGA ILAEVIGTTL MKFSEGFTRL WPSVGTIICY CASFWLLAQT LAYIPTGIAY
AIWSGVGIVL ISLLSWGFFG QRLDLPAIIG MMLICAGVLI INLLSRSTPH
Length

110

Mol. Wt

11.958 kDa

pI

8.0 (calculated)

Extinction coefficient

29,450 - 29,825 (calc based on 5 Y, 4 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

2..94

PF00893 Small Multidrug Resistance protein

PMID:19920124[12]

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tmhmm.php?gene=emrE&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=emrE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128526

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948442

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001863

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P23895

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10629

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10629

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948442

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000622

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0623

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Morimyo, M et al. (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity. Nucleic Acids Res. 20 3159-65 PubMed
  6. ↑ 6.0 6.1 Winstone, TL et al. (2002) Optimization of expression and the purification by organic extraction of the integral membrane protein EmrE. Protein Expr. Purif. 26 111-21 PubMed
  7. ↑ 7.0 7.1 Yerushalmi, H et al. (1995) EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents. J. Biol. Chem. 270 6856-63 PubMed
  8. ↑ Schwaiger, M et al. (1998) NMR investigation of the multidrug transporter EmrE, an integral membrane protein. Eur. J. Biochem. 254 610-9 PubMed
  9. ↑ Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  11. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  12. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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