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Expression|

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Standard Name	emrB
Gene Synonym(s)	ECK2680, b2686, JW2661^[1], JW2661
Product Desc.	EmrB multidrug MFS transporter^[2]^[3]; Component of EmrAB-TolC Multidrug Efflux Transport System^[3] EmrAB-TolC multidrug resistance pump; energy-dependent efflux of octane, thiolactomycin, and uncouplers DNP, CCCP, and FCCP; inner membrane-spanning translocase^[4]
Product Synonyms(s)	multidrug efflux system protein^[1], B2686^[2]^[1], EmrB^[2]^[1] , ECK2680, JW2661, b2686
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): emrRAB^[2]
Regulation/Activity
Quick Links
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Notes

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Major Facilitator Superfamily (MFS).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	emrB
Mnemonic	Efflux-multidrug resistance
Synonyms	ECK2680, b2686, JW2661^[1], JW2661
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.58 minutes	MG1655: 2810638..2812176 <gbrowseImage> name=NC_000913:2810638..2812176 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2707475..2709013 <gbrowseImage> name=NC_012967:2707475..2709013 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2696450..2697988 <gbrowseImage> name=NC_012759:2696450..2697988 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2811272..2812810 <gbrowseImage> name=NC_007779:2811272..2812810 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2903180..2904718 <gbrowseImage> name=NC_010473:2903180..2904718 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔemrB (Keio:JW2661)	deletion	deletion			PMID:16738554	Shigen CGSC10099^[5]
ΔemrB767::kan					PMID:16738554	CGSC:100700
emrB in mutant strain CDM5			Resistant to	Resistant to Thiolactomycin in vivo. It is still sensitive in Vitro.	PMID:8509326	Strain: CDM5 Parental Strain: UB1005	See figure 2.
pEMR2.1 in strain RP437			Resistant to	This milticopy plasmid in strain RP437, emrA ⁺ & emrB ⁺ in pUC18, is resistant to Nalidixic acid	PMID:1409590	Parental: RP437 CGSC Expiramental: RP437 with plasmid pEMR2.1
pEMR2.1 in strain RP437			Resistant to	This milticopy plasmid in strain RP437, emrA ⁺ & emrB ⁺ in pUC18, is resistant to Carbonyl cyanide m-chlorophenyl hydrazone (CCCP).	PMID:1409590	Parental: RP437 CGSC Expiramental: RP437 with plasmid pEMR2.1
emrB::Tn10(Kan)			Resistant to	Resistance to Thiolactomycin	PMID:8509326	Experimental Strain: SJ261 Parental Strain: UB1005	See table 2
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2661	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAACAGCAAAAACCGCTGGA Primer 2:CCGTGCGCACCGCCTCCGCCGCC
8G10	Kohara Phage	Genobase	PMID:3038334
23B7	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: 1% ^[6]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 59% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11439	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11439	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001403	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947167	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1409	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008840	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	EmrB
Synonyms	multidrug efflux system protein^[1], B2686^[2]^[1], EmrB^[2]^[1] , ECK2680, JW2661, b2686
Product description	EmrB multidrug MFS transporter^[2]^[3]; Component of EmrAB-TolC Multidrug Efflux Transport System^[3] EmrAB-TolC multidrug resistance pump; energy-dependent efflux of octane, thiolactomycin, and uncouplers DNP, CCCP, and FCCP; inner membrane-spanning translocase^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004638	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004638	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011701	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of EmrAB-TolC Multidrug Efflux Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQQQKPLEGA QLVIMTIALS LATFMQVLDS TIANVAIPTI AGNLGSSLSQ GTWVITSFGV ANAISIPLTG WLAKRVGEVK LFLWSTIAFA IASWACGVSS SLNMLIFFRV IQGIVAGPLI PLSQSLLLNN YPPAKRSIAL ALWSMTVIVA PICGPILGGY ISDNYHWGWI FFINVPIGVA VVLMTLQTLR GRETRTERRR IDAVGLALLV IGIGSLQIML DRGKELDWFS SQEIIILTVV AVVAICFLIV WELTDDNPIV DLSLFKSRNF TIGCLCISLA YMLYFGAIVL LPQLLQEVYG YTATWAGLAS APVGIIPVIL SPIIGRFAHK LDMRRLVTFS FIMYAVCFYW RAYTFEPGMD FGASAWPQFI QGFAVACFFM PLTTITLSGL PPERLAAASS LSNFTRTLAG SIGTSITTTM WTNRESMHHA QLTESVNPFN PNAQAMYSQL EGLGMTQQQA SGWIAQQITN QGLIISANEI FWMSAGIFLV LLGLVWFAKP PFGAGGGGGG AH
Length	512
Mol. Wt	55.626 kDa
pI	8.1 (calculated)
Extinction coefficient	104,390 - 105,265 (calc based on 11 Y, 16 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	19..414	PF07690 Major Facilitator Superfamily		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=emrB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130598	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947167	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008840	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEJ0	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11439	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11439	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947167	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001403	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1409	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	181	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	40	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	59	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
emrRAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2810618..2810658 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2686 (EcoliWiki Page)	NCBI GEO profiles for emrB
microarray	GenExpDB:b2686 (EcoliWiki Page)	Summary of data for emrB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to emrB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11439	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1409	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2686
EcoGene	EcoGene:EG11439	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001403	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008840	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000018326 (score: 1.000; bootstrap: 100%) ENSANGP00000020909 (score: 0.369) ENSANGP00000017425 (score: 0.254) ENSANGP00000022354 (score: 0.208) ENSANGP00000003717 (score: 0.162) ENSANGP00000018243 (score: 0.161) ENSANGP00000024634 (score: 0.137) ENSANGP00000022243 (score: 0.101) ENSANGP00000011833 (score: 0.081) ENSANGP00000009351 (score: 0.070) ENSANGP00000021350 (score: 0.061)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0191430 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YKR105C (score: 1.000; bootstrap: 77%) YCL069W (score: 0.764) YGR224W (score: 0.428) YPR198W (score: 0.305)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC16A37c (score: 1.000; bootstrap: 92%) SPAC3H16c (score: 0.323) SPAC13992 (score: 0.314) SPBC3E76c (score: 0.057)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000051762 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	EMRB	From SHIGELLACYC
E. coli O157	EMRB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:103473
Pfam (EcoliWiki Page)	PF07690 Major Facilitator Superfamily
EcoCyc	EcoCyc:EG11439	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11439	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001403	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1409	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008840	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

emrB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to emrB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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