emrB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
emrB |
|---|---|
| Mnemonic |
Efflux-multidrug resistance |
| Synonyms |
ECK2680, b2686, JW2661[1], JW2661 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
60.58 minutes |
MG1655: 2810638..2812176 |
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NC_012967: 2707475..2709013 |
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NC_012759: 2696450..2697988 |
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W3110 |
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W3110: 2811272..2812810 |
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DH10B: 2903180..2904718 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔemrB (Keio:JW2661) |
deletion |
deletion |
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ΔemrB767::kan |
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emrB in mutant strain CDM5 |
Resistant to |
Resistant to Thiolactomycin in vivo. It is still sensitive in Vitro. |
Strain: CDM5 Parental Strain: UB1005 |
See figure 2. | |||
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pEMR2.1 in strain RP437 |
Resistant to |
This milticopy plasmid in strain RP437, emrA + & emrB + in pUC18, is resistant to Nalidixic acid |
Parental: RP437 CGSC Expiramental: RP437 with plasmid pEMR2.1 |
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pEMR2.1 in strain RP437 |
Resistant to |
This milticopy plasmid in strain RP437, emrA + & emrB + in pUC18, is resistant to Carbonyl cyanide m-chlorophenyl hydrazone (CCCP). |
Parental: RP437 CGSC Expiramental: RP437 with plasmid pEMR2.1 |
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emrB::Tn10(Kan) |
Resistant to |
Resistance to Thiolactomycin |
Experimental Strain: SJ261 Parental Strain: UB1005 |
See table 2 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2661 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCAACAGCAAAAACCGCTGGA Primer 2:CCGTGCGCACCGCCTCCGCCGCC | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 1% [8] | ||
|
Linked marker |
est. P1 cotransduction: 59% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Furukawa, H et al. (1993) Thiolactomycin resistance in Escherichia coli is associated with the multidrug resistance efflux pump encoded by emrAB. J. Bacteriol. 175 3723-9 PubMed
- ↑ 5.0 5.1 Lomovskaya, O & Lewis, K (1992) Emr, an Escherichia coli locus for multidrug resistance. Proc. Natl. Acad. Sci. U.S.A. 89 8938-42 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
