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Gene |

Product(s) |

Expression|

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Standard Name	efp
Gene Synonym(s)	ECK4141, b4147, JW4107^[1], JW4107
Product Desc.	protein chain elongation factor EF-P^[2]^[3] Elongation factor EF-P, stimulates peptide bond formation^[4]
Product Synonyms(s)	Elongation factor EF-P^[1], B4147^[2]^[1], Efp^[2]^[1] , ECK4141, JW4107, b4147
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): efp^[2]
Regulation/Activity
Quick Links
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Notes

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efp has been reported to be an essential gene (Aoki, 1997) and to be a non-essential gene (Baba, 2006). Binds TrxA (Kumar, 2004).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	efp
Mnemonic	Elongation Factor-Peptidyltransferase
Synonyms	ECK4141, b4147, JW4107^[1], JW4107
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	94.27 minutes	MG1655: 4373722..4374288 <gbrowseImage> name=NC_000913:4373722..4374288 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4354410..4354976 <gbrowseImage> name=NC_012967:4354410..4354976 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4312457..4313023 <gbrowseImage> name=NC_012759:4312457..4313023 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4380379..4380945 <gbrowseImage> name=NC_007779:4380379..4380945 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4474084..4474650 <gbrowseImage> name=NC_010473:4474084..4474650 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4373725	Edman degradation	PMID:1956781 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δefp (Keio:JW4107)	deletion	deletion	PMID:16738554	Shigen CGSC10957^[5]
Δefp-772::kan			PMID:16738554	CGSC:100688
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4107	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAACGTACTATAGCAACGA Primer 2:CCtTTCACGCGAGAGACGTATTC
9B1	Kohara Phage	Genobase	PMID:3038334
1D4	Kohara Phage	Genobase	PMID:3038334
3H6	Kohara Phage	Genobase	PMID:3038334
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 54% ^[6] Synonyms:zjd-2231::Tn10
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 8% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12099	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12099	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002004	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948661	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2023	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013583	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Efp
Synonyms	Elongation factor EF-P^[1], B4147^[2]^[1], Efp^[2]^[1] , ECK4141, JW4107, b4147
Product description	protein chain elongation factor EF-P^[2]^[3] Elongation factor EF-P, stimulates peptide bond formation^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003746	translation elongation factor activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00141	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003746	translation elongation factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001059	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003746	translation elongation factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011768	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003746	translation elongation factor activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0251	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00141	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011768	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:1105576	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0648	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006414	translational elongation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00141	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006414	translational elongation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001059	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006414	translational elongation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011768	P	Seeded from EcoCyc (v14.0)	complete
	GO:0043022	ribosome binding	PMID:18201202	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MATYYSNDFR AGLKIMLDGE PYAVEASEFV KPGKGQAFAR VKLRRLLTGT RVEKTFKSTD SAEGADVVDM NLTYLYNDGE FWHFMNNETF EQLSADAKAI GDNAKWLLDQ AECIVTLWNG QPISVTPPNF VELEIVDTDP GLKGDTAGTG GKPATLSTGA VVKVPLFVQI GEVIKVDTRS GEYVSRVK
Length	188
Mol. Wt	20.59 kDa
pI	4.8 (calculated)
Extinction coefficient	25,440 - 25,565 (calc based on 6 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A6N4
Domain	69..123	PF01132 Elongation factor P (EF-P) OB domain		PMID:19920124
Domain	5..62	PF08207 Elongation factor P (EF-P) KOW-like domain		PMID:19920124
Domain	131..186	PF09285 Elongation factor P, C-terminal		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=efp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131972	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948661	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013583	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6N4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12099	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12099	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948661	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002004	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2023	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	5.85E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 EMG2	1600	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: M148	PMID: 9298646
Protein	E. coli K-12 MG1655	41819	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	7562	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	19159	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
efp
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4373702..4373742 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4147 (EcoliWiki Page)	NCBI GEO profiles for efp
microarray	GenExpDB:b4147 (EcoliWiki Page)	Summary of data for efp from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4373161..4373340) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ08; Well:H8^[7]
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Notes

Accessions Related to efp Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12099	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2023	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4147
EcoGene	EcoGene:EG12099	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002004	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013583	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000002223 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G08740 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0217192 (score: 1.000; bootstrap: 100%) DDB0168187 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000041614 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	EFP	From SHIGELLACYC
E. coli O157	EFP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01132 Elongation factor P (EF-P) OB domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:50104
Superfamily (EcoliWiki Page)	SUPERFAMILY:50249
Superfamily (EcoliWiki Page)	SUPERFAMILY:50249
Pfam (EcoliWiki Page)	PF08207 Elongation factor P (EF-P) KOW-like domain
Pfam (EcoliWiki Page)	PF09285 Elongation factor P, C-terminal
PFAM (EcoliWiki Page)	PFAM:PF01132
EcoCyc	EcoCyc:EG12099	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12099	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002004	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2023	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013583	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

efp:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to efp Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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