edd:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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Standard name

edd

Mnemonic

Entner-Douderoff dehydratase

Synonyms

ECK1852, b1851, JW1840[1], JW1840

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

41.62 minutes 

MG1655: 1932628..1930817
<gbrowseImage> name=NC_000913:1930817..1932628 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1913180..1911369
<gbrowseImage> name=NC_012967:1911369..1913180 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1822876..1824687
<gbrowseImage> name=NC_012759:1822876..1824687 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1936318..1934507
<gbrowseImage> name=NC_007779:1934507..1936318 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2023199..2021388
<gbrowseImage> name=NC_010473:2021388..2023199 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

edd(del) (Keio:JW1840)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC9536[3]

edd-1

CGSC:8107

edd-776(del)::kan

PMID:16738554[2]

CGSC:100686

edd(del)

deletion

deletion

Colony Morphology

Mutations were non-motile after growth in LB and failed to spread on agar, figure 7.

PMID:16299298[4]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1840

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAATCCACAATTGTTACGCGT

Primer 2:CCAAAAGTGATACAGGTTGCGCC

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[3]

est. P1 cotransduction: 98% [6]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[3]

est. P1 cotransduction: 5% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10257

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10257

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000251

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946362

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0253

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006166

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Leatham, MP et al. (2005) Mouse intestine selects nonmotile flhDC mutants of Escherichia coli MG1655 with increased colonizing ability and better utilization of carbon sources. Infect. Immun. 73 8039-49 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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