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Gene |

Product(s) |

Expression|

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Standard Name	eamA
Gene Synonym(s)	ECK1526, b1533, JW5250, ydeD^[1], ydeD
Product Desc.	O-acetylserine/cysteine export protein^[2]^[3] Efflux pump for cysteine and O-acetyl-L-serine^[4]
Product Synonyms(s)	cysteine and O-acetyl-L-serine efflux system^[1], B1533^[2]^[1], EamA^[2]^[1], YdeD^[2]^[1] , ECK1526, JW5250, ydeD, b1533
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): eamA^[2]
Regulation/Activity
Quick Links
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Notes

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EamA belongs to the EamA/RhaT transporter family, DMT superfamily. Dasler et al. used start codon mutagenesis to verify this start codon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	eamA
Mnemonic	Efflux of amino acid metabolites
Synonyms	ECK1526, b1533, JW5250, ydeD^[1], ydeD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	34.88 minutes	MG1655: 1619161..1618262 <gbrowseImage> name=NC_000913:1618262..1619161 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1588903..1588004 <gbrowseImage> name=NC_012967:1588004..1588903 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1510321..1511220 <gbrowseImage> name=NC_012759:1510321..1511220 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1622851..1621952 <gbrowseImage> name=NC_007779:1621952..1622851 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1708786..1707887 <gbrowseImage> name=NC_010473:1707887..1708786 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔeamA (Keio:JW5250)	deletion	deletion	PMID:16738554	Shigen CGSC11270^[5]
ydeD::Tn5KAN-I-SceI (FB20352)	Insertion at nt 304 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20352	contains pKD46
ydeD::Tn5KAN-I-SceI (FB20353)	Insertion at nt 304 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20353	does not contain pKD46
ΔeamA754::kan			PMID:16738554	CGSC:100672
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5250	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCGCGAAAAGATGGGGTGTT Primer 2:CCACTTCCCACCTTTACCGCTTT
2H2	Kohara Phage	Genobase	PMID:3038334
2B2	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdh-925::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11639	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11639	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001585	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946081	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1594	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005116	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	EamA
Synonyms	cysteine and O-acetyl-L-serine efflux system^[1], B1533^[2]^[1], EamA^[2]^[1], YdeD^[2]^[1] , ECK1526, JW5250, ydeD, b1533
Product description	O-acetylserine/cysteine export protein^[2]^[3] Efflux pump for cysteine and O-acetyl-L-serine^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0029	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000620	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 10 predicted transmembrane domains	Daley et al. (2005) ^[7]
membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLAGLRFMLV AFPAIFFVAR PKVPLNLLLG YGLTISFAQF AFLFCAINFG MPAGLASLVL QAQAFFTIML GAFTFGERLH GKQLAGIALA IFGVLVLIED SLNGQHVAML GFMLTLAAAF SWACGNIFNK KIMSHSTRPA VMSLVIWSAL IPIIPFFVAS LILDGSATMI HSLVTIDMTT ILSLMYLAFV ATIVGYGIWG TLLGRYETWR VAPLSLLVPV VGLASAALLL DERLTGLQFL GAVLIMTGLY INVFGLRWRK AVKVGS
Length	266
Mol. Wt	28.728 kDa
pI	10.2 (calculated)
Extinction coefficient	34,950 - 35,200 (calc based on 5 Y, 5 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	14..131	PF00892 EamA-like transporter family	PMID:19920124
Domain	153..287	PF00892 EamA-like transporter family	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=eamA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111291	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946081	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005116	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31125	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11639	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11639	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946081	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001585	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1594	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	79	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	156	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	52	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
eamA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1619141..1619181 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1533 (EcoliWiki Page)	NCBI GEO profiles for eamA
microarray	GenExpDB:b1533 (EcoliWiki Page)	Summary of data for eamA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to eamA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11639	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1594	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1533
EcoGene	EcoGene:EG11639	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001585	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005116	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000002373 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000029839 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	YDED	From SHIGELLACYC
E. coli O157	YDED	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00892 EamA-like transporter family
Pfam (EcoliWiki Page)	PF00892 EamA-like transporter family
Superfamily (EcoliWiki Page)	SUPERFAMILY:103481
Superfamily (EcoliWiki Page)	SUPERFAMILY:103481
EcoCyc	EcoCyc:EG11639	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11639	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001585	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1594	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005116	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

eamA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to eamA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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