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Product(s) |

Expression|

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Standard Name	dhaL
Gene Synonym(s)	ECK1187, b1199, JW5186, ycgS, dhaK2, ptsD, ysgS^[1]^[2]
Product Desc.	dihydroxyacetone kinase subunit L^[2]^[3]; Component of dihydroxyacetone kinase^[3] Dihydroxyacetone kinase, PTS-dependent ADP-binding subunit II; DhaK(2):DhaL:DhaM(2) complex^[4]
Product Synonyms(s)	dihydroxyacetone kinase, C-terminal domain^[1], B1199^[2]^[1], YcgS^[2]^[1], DhaL^[2]^[1], YsgS^[2]^[1] , dhaK2, ECK1187, JW5186, ptsD, ycgS, b1199
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dhaKLM^[2]
Regulation/Activity
Quick Links
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Notes

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DhaL is similar to C-terminal half of single protein DHA kinases. Phosphoryl donor is DhaM, not ATP. DhaK is a activator of DhaR.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	dhaL
Mnemonic	Dihydroxyacetone
Synonyms	ECK1187, b1199, JW5186, ycgS, dhaK2, ptsD, ysgS^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	26.91 minutes	MG1655: 1248980..1248348 <gbrowseImage> name=NC_000913:1248348..1248980 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1249907..1249275 <gbrowseImage> name=NC_012967:1249275..1249907 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1137069..1137701 <gbrowseImage> name=NC_012759:1137069..1137701 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1251334..1250702 <gbrowseImage> name=NC_007779:1250702..1251334 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1289221..1288589 <gbrowseImage> name=NC_010473:1288589..1289221 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔdhaL (Keio:JW5186)	deletion	deletion	PMID:16738554	Shigen CGSC11227^[5]
ΔdhaL789::kan			PMID:16738554	CGSC:100594
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5186	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCACTGAGCAGAACTCAAAT Primer 2:CCtTCTTTTGCGGCTAACGCCAA
7C10	Kohara Phage	Genobase	PMID:3038334
12A3	Kohara Phage	Genobase	PMID:3038334
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 61% ^[6]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 7% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6626	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13900	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003221	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945748	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3659	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004026	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DhaL
Synonyms	dihydroxyacetone kinase, C-terminal domain^[1], B1199^[2]^[1], YcgS^[2]^[1], DhaL^[2]^[1], YsgS^[2]^[1] , dhaK2, ECK1187, JW5186, ptsD, ycgS, b1199
Product description	dihydroxyacetone kinase subunit L^[2]^[3]; Component of dihydroxyacetone kinase^[3] Dihydroxyacetone kinase, PTS-dependent ADP-binding subunit II; DhaK(2):DhaL:DhaM(2) complex^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004371	glycerone kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004007	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006071	glycerol metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004007	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006071	glycerol metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0319	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016772	transferase activity, transferring phosphorus-containing groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012737	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004371	glycerone kinase activity	PMID:11350937	IPI: Inferred from Physical Interaction	EcoCyc:EG12399 EcoCyc:G6627	F		complete
Contributes to	GO:0016740	transferase activity	PMID:11350937	IDA: Inferred from Direct Assay		F	See fig 4C	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of dihydroxyacetone kinase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSLSRTQIVN WLTRCGDIFS TESEYLTGLD REIGDADHGL NMNRGFSKVV EKLPAIADKD IGFILKNTGM TLLSSVGGAS GPLFGTFFIR AAQATQARQS LTLEELYQMF RDGADGVISR GKAEPGDKTM CDVWVPVVES LRQSSEQNLS VPVALEAASS IAESAAQSTI TMQARKGRAS YLGERSIGHQ DPGATSVMFM MQMLALAAKE
Length	210
Mol. Wt	22.631 kDa
pI	5.2 (calculated)
Extinction coefficient	15,470 - 15,720 (calc based on 3 Y, 2 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	32..206	PF02734 DAK2 domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dhaL taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129162	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945748	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004026	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76014	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6626	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13900	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945748	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003221	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3659	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	285	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	410	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	357	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
dhaKLM
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1248960..1249000 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1199 (EcoliWiki Page)	NCBI GEO profiles for dhaL
microarray	GenExpDB:b1199 (EcoliWiki Page)	Summary of data for dhaL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to dhaL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6626	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3659	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1199
EcoGene	EcoGene:EG13900	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003221	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004026	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Gallus gallus	ENSGALP00000023496 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S1286	From SHIGELLACYC
E. coli O157	Z1970	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02734 DAK2 domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:101473
EcoCyc	EcoCyc:G6626	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13900	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003221	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3659	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004026	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

dhaL:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dhaL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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