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Standard Name	dacC
Gene Synonym(s)	ECK0829, b0839, JW0823, pbp^[1]
Product Desc.	penicillin-binding protein 6^[2]^[3] D-alanine D-alanine carboxypeptidase PBP6; penicillin-binding protein 6^[4]
Product Synonyms(s)	D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a)^[1], B0839^[2]^[1], DacC^[2]^[1], D-alanyl-D-alanine carboxypeptidase^[2]^[1] , ECK0829, JW0823, b0839
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dacC^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name	dacC
Mnemonic	D-Alanine carboxypeptidase
Synonyms	ECK0829, b0839, JW0823, pbp^[1]
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	18.97 minutes	MG1655: 879950..881152 <gbrowseImage> name=NC_000913:879950..881152 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 873279..874481 <gbrowseImage> name=NC_012967:873279..874481 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 782918..784120 <gbrowseImage> name=NC_012759:782918..784120 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 881149..882351 <gbrowseImage> name=NC_007779:881149..882351 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 933878..935080 <gbrowseImage> name=NC_010473:933878..935080 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	880031	Edman degradation	PMID:7042389
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔdacC (Keio:JW0823)	deletion	deletion	PMID:16738554	Shigen CGSC8863^[5]
ΔdacC739::kan			PMID:16738554	CGSC:100520
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW0823	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACGCAATACTCCTCTCTCCT Primer 2:CCAGAGAACCAGCTGCCGAACCA
3H12	Kohara Phage	Genobase	PMID:3038334
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: 5% ^[6] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 2% ^[6] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc	EcoCyc:EG10203	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10203	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000197	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945455	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0199	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002860	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DacC
Synonyms	D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a)^[1], B0839^[2]^[1], DacC^[2]^[1], D-alanyl-D-alanine carboxypeptidase^[2]^[1] , ECK0829, JW0823, b0839
Product description	penicillin-binding protein 6^[2]^[3] D-alanine D-alanine carboxypeptidase PBP6; penicillin-binding protein 6^[4]
EC number (for enzymes)	3.4.16.4^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004180	carboxypeptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0121	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005887	integral to plasma membrane	PMID:319999^[7]	IDA: Inferred from Direct Assay		C		complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001967	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012907	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015956	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018044	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0133	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001967	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012907	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018044	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.4.16.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009252	peptidoglycan biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0573	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTQYSSLLRG LAAGSAFLFL FAPTAFAAEQ TVEAPSVDAR AWILMDYASG KVLAEGNADE KLDPASLTKI MTSYVVGQAL KADKIKLTDM VTVGKDAWAT GNPALRGSSV MFLKPGDQVS VADLNKGVII QSGNDACIAL ADYVAGSQES FIGLMNGYAK KLGLTNTTFQ TVHGLDAPGQ FSTARDMALL GKALIHDVPE EYAIHKEKEF TFNKIRQPNR NRLLWSSNLN VDGMKTGTTA GAGYNLVASA TQGDMRLISV VLGAKTDRIR FNESEKLLTW GFRFFETVTP IKPDATFVTQ RVWFGDKSEV NLGAGEAGSV TIPRGQLKNL KASYTLTEPQ LTAPLKKGQV VGTIDFQLNG KSIEQRPLIV MENVEEGGFF GRVWDFVMMK FHQWFGSWFS
Length	400
Mol. Wt	43.609 kDa
pI	8.1 (calculated)
Extinction coefficient	55,920 - 56,045 (calc based on 8 Y, 8 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-27	UniProt Manual:Signal Peptides	UniProt:P08506
Modification Site	108	phosphorylation site at S108	probability less than 75%	PMID:17938405^[8]
Modification Site	109	phosphorylation site at S109	probability less than 75%	PMID:17938405^[8]
Domain	28..266	PF00768 D-alanyl-D-alanine carboxypeptidase		PMID:19920124^[9]
Domain	285..376	PF07943 Penicillin-binding protein 5, C-terminal domain		PMID:19920124^[9]
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<motif_map/>

Structure
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Structures

<beststructure> gene=dacC taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Structures described by Chen et al (2009)^[10]

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128807	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945455	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002860	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P08506	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10203	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10203	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945455	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000197	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0199	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	740	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	876	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	630	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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Transcription unit(s)
dacC
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:879930..879970 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0839 (EcoliWiki Page)	NCBI GEO profiles for dacC
microarray	GenExpDB:b0839 (EcoliWiki Page)	Summary of data for dacC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to dacC Expression

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Database	Accession	Notes
EcoCyc	EcoCyc:EG10203	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0199	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0839
EcoGene	EcoGene:EG10203	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000197	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002860	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000051393 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	DACC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR21581:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:56601
Superfamily (EcoliWiki Page)	SUPERFAMILY:69189
Pfam (EcoliWiki Page)	PF00768 D-alanyl-D-alanine carboxypeptidase
Pfam (EcoliWiki Page)	PF07943 Penicillin-binding protein 5, C-terminal domain
EcoCyc	EcoCyc:EG10203	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10203	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000197	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0199	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002860	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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References

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↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Spratt, BG (1977) Properties of the penicillin-binding proteins of Escherichia coli K12,. Eur. J. Biochem. 72 341-52 PubMed
↑ ^8.0 ^8.1 Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
↑ ^9.0 ^9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
↑ Chen, Y et al. (2009) Crystal structures of penicillin-binding protein 6 from Escherichia coli. J. Am. Chem. Soc. 131 14345-54 PubMed

dacC:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

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Sequence Features

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Alleles and Phenotypes

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Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

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Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dacC Expression

Notes

Links

Evolution

Homologs in Other Organisms

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Notes

Families

Notes

Links

References

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