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Product(s) |

Expression|

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Standard Name	cysQ
Gene Synonym(s)	ECK4210, b4214, JW4172, amt, amtA^[1], amtA
Product Desc.	affects pool of 3'-phosphoadenosine-5'-phosphosulfate in pathway of sulfite synthesis^[2]^[3] 3'-phosphoadenosine 5'-phosphate (pAp) phosphatase; converts pAp to AMP; inhibited by lithium and calcium, leading to pAP accumulation; probable PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase: probably converts PAPS to^[4]
Product Synonyms(s)	PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase^[1], B4214^[2]^[1], Amt^[2]^[1], AmtA^[2]^[1], CysQ^[2]^[1] , amt, amtA, ECK4210, JW4172, b4214
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cysQ^[2]
Regulation/Activity
Quick Links
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Notes

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CysQ is a main target of lithium toxicity in vivo. CysQ (AmtA) was originally thought to be involved in ammonium transport. CysQ may be part of a futile sulfur cycle with APS kinase CysC.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cysQ
Mnemonic	Cysteine
Synonyms	ECK4210, b4214, JW4172, amt, amtA^[1], amtA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	95.58 minutes, 95.58 minutes	MG1655: 4434778..4435518 <gbrowseImage> name=NC_000913:4434778..4435518 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4421749..4422489 <gbrowseImage> name=NC_012967:4421749..4422489 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4373513..4374253 <gbrowseImage> name=NC_012759:4373513..4374253 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4441435..4442175 <gbrowseImage> name=NC_007779:4441435..4442175 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
Strain DH10B		P68F	Auxotrophies	Nonsynonomous mutation	PMID:18245285	ASAP	This is a SNP in E. coli K-12 Strain DH10B
ΔcysQ (Keio:JW4172)	deletion	deletion	Auxotrophies		PMID:16738554	Shigen CGSC11005^[5]
ΔcysQ763::kan	deletion	deletion	Auxotrophies		PMID:16738554	CGSC:100506
cysQ::Tn5tac1			Growth Phenotype	does not require cysteine for growth in anaerobic conditions, mutation is leaky but not revertible	PMID:1729235		IPTG-induced transcription from Tn5tac1 turns off cysQ expression
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4172	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTAGATCAAGTATGCCAGCT Primer 2:CCGTAAATAGACACTCTGAACCC
7E9	Kohara Phage	Genobase	PMID:3038334
5B5	Kohara Phage	Genobase	PMID:3038334
E1H3	Kohara Phage	Genobase	PMID:3038334
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 75% ^[6]
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[5]	est. P1 cotransduction: 71% ^[6] Synonyms:zjh-901::Tn10, zjg-920::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10043	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10043	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000039	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948728	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0041	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013786	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CysQ
Synonyms	PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase^[1], B4214^[2]^[1], Amt^[2]^[1], AmtA^[2]^[1], CysQ^[2]^[1] , amt, amtA, ECK4210, JW4172, b4214
Product description	affects pool of 3'-phosphoadenosine-5'-phosphosulfate in pathway of sulfite synthesis^[2]^[3] 3'-phosphoadenosine 5'-phosphate (pAp) phosphatase; converts pAp to AMP; inhibited by lithium and calcium, leading to pAP accumulation; probable PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase: probably converts PAPS to^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006240	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004437	inositol or phosphatidylinositol phosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000760	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004437	inositol or phosphatidylinositol phosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020550	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004437	inositol or phosphatidylinositol phosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020583	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008441	3'(2'),5'-bisphosphate nucleotidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.7	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:17616624	IC: Inferred by Curator		C		Missing: with/from
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006240	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
periplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLDQVCQLAR NAGDAIMQVY DGTKPMDVVS KADNSPVTAA DIAAHTVIMD GLRTLTPDVP VLSEEDPPGW EVRQHWQRYW LVDPLDGTKE FIKRNGEFTV NIALIDHGKP ILGVVYAPVM NVMYSAAEGK AWKEECGVRK QIQVRDARPP LVVISRSHAD AELKEYLQQL GEHQTTSIGS SLKFCLVAEG QAQLYPRFGP TNIWDTAAGH AVAAAAGAHV HDWQGKPLDY TPRESFLNPG FRVSIY
Length	246
Mol. Wt	27.174 kDa
pI	5.9 (calculated)
Extinction coefficient	44,920 - 45,295 (calc based on 8 Y, 6 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..243	PF00459 Inositol monophosphatase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cysQ taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132036	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948728	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013786	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P22255	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10043	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10043	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948728	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000039	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0041	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.12E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	902	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	533	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	667	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cysQ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4434758..4434798 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4214 (EcoliWiki Page)	NCBI GEO profiles for cysQ
microarray	GenExpDB:b4214 (EcoliWiki Page)	Summary of data for cysQ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4434065..4434386) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ05; Well:E5^[7]
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Notes

Accessions Related to cysQ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10043	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0041	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4214
EcoGene	EcoGene:EG10043	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000039	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013786	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001820 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00026489 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00012390 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0189923 (score: 1.000; bootstrap: 56%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0030465 (score: 1.000; bootstrap: 100%) FBgn0039698 (score: 0.105)	From Inparanoid:20070104
Drosophila pseudoobscura	GA13929-PA (score: 1.000; bootstrap: 100%) GA20589-PA (score: 0.115)	From Inparanoid:20070104
Oryza gramene	Q6Z413 (score: 1.000; bootstrap: 99%) Q6Z412 (score: 0.568) Q948F9 (score: 0.062) Q6K841 (score: 0.052)	From Inparanoid:20070104
Saccharomyces cerevisiae	YOL064C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC17534 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00020318001 (score: 1.000; bootstrap: 100%) GSTENP00013621001 (score: 0.108) GSTENP00006004001 (score: 0.073)	From Inparanoid:20070104
Shigella flexneri	CYSQ	From SHIGELLACYC
E. coli O157	CYSQ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00459 Inositol monophosphatase family
Panther (EcoliWiki Page)	PTHR20854:SF5
Superfamily (EcoliWiki Page)	SUPERFAMILY:56655
EcoCyc	EcoCyc:EG10043	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10043	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000039	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0041	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013786	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

cysQ:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cysQ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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