{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	cysE
Gene Synonym(s)	ECK3597, b3607, JW3582^[1], JW3582
Product Desc.	serine acetyltransferase^[2]^[3]; Component of serine acetyltransferase^[3]; cysteine synthase^[2]; bifunctional CysEK cysteine biosynthesis complex^[3] Cysteine synthase, serine acetyltransferase (SAT); first step in cysteine synthesis; feedback inhibited by cysteine.hexameric^[4]
Product Synonyms(s)	serine acetyltransferase^[1], B3607^[2]^[1], CysE^[2]^[1] , ECK3597, JW3582, b3607
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cysE^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

CysE forms a bienzyme complex with CysK. CysE contains 13 hexapeptide repeats.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cysE
Mnemonic	Cysteine
Synonyms	ECK3597, b3607, JW3582^[1], JW3582
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	81.47 minutes	MG1655: 3780585..3779764 <gbrowseImage> name=NC_000913:3779764..3780585 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3723328..3722507 <gbrowseImage> name=NC_012967:3722507..3723328 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3668090..3668911 <gbrowseImage> name=NC_012759:3668090..3668911 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3857853..3858674 <gbrowseImage> name=NC_007779:3857853..3858674 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3878162..3877341 <gbrowseImage> name=NC_010473:3877341..3878162 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔcysE (Keio:JW3582)	deletion	deletion	Auxotrophies		PMID:16738554	Shigen CGSC10639^[5]
cysE::Tn5KAN-I-SceI (FB21338)	Insertion at nt 108 in Minus orientation		Auxotrophies		PMID:15262929	E. coli Genome Project:FB21338	does not contain pKD46
cysE::Tn5KAN-I-SceI (FB21339)	Insertion at nt 108 in Minus orientation		Auxotrophies		PMID:15262929	E. coli Genome Project:FB21339	contains pKD46
cysE51			Auxotrophies			CGSC:5852
cysE50			Resistant to	increased resistance to novobiocin	PMID:1886615	CGSC:5859
cysE52			Auxotrophies			CGSC:5861
cysE8			Auxotrophies			CGSC:13142
cysE157(fbR)			Auxotrophies	feedback resistant	PMID:3309158	CGSC:73928
ΔcysE720::kan	deletion	deletion	Auxotrophies		PMID:16738554	CGSC:100488
cysE in mutant strain SY400			Resistant to	Resistance to Novobiocin	PMID:1886615	Strain: SY400	See figure 1
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3582	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCGTGTGAAGAACTGGAAAT Primer 2:CCGATCCCATCCCCATACTCAAA
5D2	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 11% ^[6]
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10187	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10187	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000182	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948126	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0184	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011793	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CysE
Synonyms	serine acetyltransferase^[1], B3607^[2]^[1], CysE^[2]^[1] , ECK3597, JW3582, b3607
Product description	serine acetyltransferase^[2]^[3]; Component of serine acetyltransferase^[3]; cysteine synthase^[2]; bifunctional CysEK cysteine biosynthesis complex^[3] Cysteine synthase, serine acetyltransferase (SAT); first step in cysteine synthesis; feedback inhibited by cysteine.hexameric^[4]
EC number (for enzymes)	2.3.1.30^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006535	cysteine biosynthetic process from serine	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005881	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006535	cysteine biosynthetic process from serine	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010493	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009001	serine O-acetyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005881	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009001	serine O-acetyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010493	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009001	serine O-acetyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.3.1.30	F	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of serine acetyltransferase	could be indirect
Protein	nadE		PMID:16606699	Experiment(s):EBI-1146234
Protein	Subunits of cysteine synthase	could be indirect
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSCEELEIVW NNIKAEARTL ADCEPMLASF YHATLLKHEN LGSALSYMLA NKLSSPIMPA IAIREVVEEA YAADPEMIAS AACDIQAVRT RDPAVDKYST PLLYLKGFHA LQAYRIGHWL WNQGRRALAI FLQNQVSVTF QVDIHPAAKI GRGIMLDHAT GIVVGETAVI ENDVSILQSV TLGGTGKSGG DRHPKIREGV MIGAGAKILG NIEVGRGAKI GAGSVVLQPV PPHTTAAGVP ARIVGKPDSD KPSMDMDQHF NGINHTFEYG DGI
Length	273
Mol. Wt	29.316 kDa
pI	6.5 (calculated)
Extinction coefficient	26,930 - 27,305 (calc based on 7 Y, 3 W, and 3 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	9..113	PF06426 Serine acetyltransferase, N-terminal		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cysE taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131478	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948126	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011793	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9D4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10187	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10187	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948126	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000182	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0184	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.15E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	817	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	524	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	574	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cysE
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:3780565..3780605 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3607 (EcoliWiki Page)	NCBI GEO profiles for cysE
microarray	GenExpDB:b3607 (EcoliWiki Page)	Summary of data for cysE from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to cysE Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10187	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0184	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3607
EcoGene	EcoGene:EG10187	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000182	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011793	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000025308 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G56760 (score: 1.000; bootstrap: 100%) AT3G13110 (score: 0.220) AT1G55920 (score: 0.198) AT4G35640 (score: 0.111) AT2G17640 (score: 0.078)	From Inparanoid:20070104
Oryza gramene	Q6ZHU3 (score: 1.000; bootstrap: 100%) Q8W0E4 (score: 0.515) Q8GZP8 (score: 0.473) Q8GZP9 (score: 0.357) Q8GZP7 (score: 0.076)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC10398 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000010757 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	CYSE	From SHIGELLACYC
E. coli O157	CYSE	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:51161
Panther (EcoliWiki Page)	PTHR23416:SF2
Pfam (EcoliWiki Page)	PF06426 Serine acetyltransferase, N-terminal
EcoCyc	EcoCyc:EG10187	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10187	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000182	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0184	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011793	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

cysE:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cysE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search