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Expression|

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Standard Name	caiB
Gene Synonym(s)	ECK0039, b0038, JW0037, yaaN^[1], yaaN
Product Desc.	CaiB^[2]^[3]; Component of γ-butyrobetainyl-CoA:carnitine CoA transferase^[2]^[3] Carnitine dehydratase; crotonobetaine reductase, CI component^[4]
Product Synonyms(s)	crotonobetainyl CoA:carnitine CoA transferase^[1], B0038^[2]^[1], YaaN^[2]^[1], CaiB^[2]^[1] , ECK0039, JW0037, yaaN, b0038
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): caiTABCDE^[2]
Regulation/Activity
Quick Links
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Notes

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Uses gamma-butyrobetainyl-CoA as co-factor.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	caiB
Mnemonic	Carnitine inducible
Synonyms	ECK0039, b0038, JW0037, yaaN^[1], yaaN
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	0.82 minutes	MG1655: 39115..37898 <gbrowseImage> name=NC_000913:37898..39115 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 37898..39115 <gbrowseImage> name=NC_012759:37898..39115 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 39115..37898 <gbrowseImage> name=NC_007779:37898..39115 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 39115..37898 <gbrowseImage> name=NC_010473:37898..39115 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	37898	Edman degradation	PMID:8188598 PMID:10209289
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
caiBT302A		T302A	(in strain: O44:K74)			Strain variation; seeded from UniProt:P31572
caiBV187A		V187A	(in strain: O44:K74)			Strain variation; seeded from UniProt:P31572
caiB(del) (Keio:JW0037)	deletion	deletion		PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0037	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGATCATCTACCCATGCCGAA Primer 2:CCGTCCTCAACTTTGGCCAGACC
22B12	Kohara Phage	Genobase	PMID:3038334
2F7	Kohara Phage	Genobase	PMID:3038334
4A3	Kohara Phage	Genobase	PMID:3038334
carB96::Tn10	Linked marker	CAG12093 = CGSC7322^[5]	est. P1 cotransduction: 83% ^[6] Synonyms:car-96::Tn10
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 13% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11559	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11559	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001512	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948997	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1520	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000136	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CaiB
Synonyms	crotonobetainyl CoA:carnitine CoA transferase^[1], B0038^[2]^[1], YaaN^[2]^[1], CaiB^[2]^[1] , ECK0039, JW0037, yaaN, b0038
Product description	CaiB^[2]^[3]; Component of γ-butyrobetainyl-CoA:carnitine CoA transferase^[2]^[3] Carnitine dehydratase; crotonobetaine reductase, CI component^[4]
EC number (for enzymes)	2.8.3.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009310	amine catabolic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003673	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01050	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003673	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008410	CoA-transferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01050	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008735	carnitine dehydratase activity	PMID:8188598	EXP: Inferred from Experiment		F		complete
	GO:0042413	carnitine catabolic process	PMID:8188598	EXP: Inferred from Experiment		P		complete
	GO:0008735	carnitine dehydratase activity	PMID:7815937	EXP: Inferred from Experiment		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of γ-butyrobetainyl-CoA:carnitine CoA transferase	could be indirect
Protein	dld		PMID:16606699	Experiment(s):EBI-1135397
Protein	rhsD		PMID:16606699	Experiment(s):EBI-1135397
Protein	sdhA		PMID:16606699	Experiment(s):EBI-1135397
Protein	rhsA		PMID:16606699	Experiment(s):EBI-1135397
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDHLPMPKFG PLAGLRVVFS GIEIAGPFAG QMFAEWGAEV IWIENVAWAD TIRVQPNYPQ LSRRNLHALS LNIFKDEGRE AFLKLMETTD IFIEASKGPA FARRGITDEV LWQHNPKLVI AHLSGFGQYG TEEYTNLPAY NTIAQAFSGY LIQNGDVDQP MPAFPYTADY FSGLTATTAA LAALHKVRET GKGESIDIAM YEVMLRMGQY FMMDYFNGGE MCPRMSKGKD PYYAGCGLYK CADGYIVMEL VGITQIEECF KDIGLAHLLG TPEIPEGTQL IHRIECPYGP LVEEKLDAWL ATHTIAEVKE RFAELNIACA KVLTVPELES NPQYVARESI TQWQTMDGRT CKGPNIMPKF KNNPGQIWRG MPSHGMDTAA ILKNIGYSEN DIQELVSKGL AKVED
Length	405
Mol. Wt	45.127 kDa
pI	4.9 (calculated)
Extinction coefficient	63,830 - 64,705 (calc based on 17 Y, 7 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	69..258	PF02515 CoA-transferase family III		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=caiB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128032	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000136	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31572	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11559	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11559	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948997	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001512	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948997	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1520	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	29	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	6^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
caiTABCDE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:39095..39135 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0038 (EcoliWiki Page)	NCBI GEO profiles for caiB
microarray	GenExpDB:b0038 (EcoliWiki Page)	Summary of data for caiB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to caiB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11559	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1520	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0038
EcoGene	EcoGene:EG11559	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001512	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000136	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Caenorhabditis elegans	WBGene00014128 (score: 1.000; bootstrap: 100%) WBGene00016034 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000017701 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	CAIB	From SHIGELLACYC
E. coli O157	CAIB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02515 CoA-transferase family III
Superfamily (EcoliWiki Page)	SUPERFAMILY:89796
EcoCyc	EcoCyc:EG11559	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11559	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001512	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1520	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000136	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

caiB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to caiB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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