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Standard Name	btuD
Gene Synonym(s)	ECK1707, b1709, JW1699^[1], JW1699
Product Desc.	BtuD^[2]^[3]; Component of vitamin B12 ABC transporter^[2]^[3]; vitamin B12 transport system^[3] B12 uptake, ABC transporter permease^[4]
Product Synonyms(s)	vitamin B12 transporter subunit : ATP-binding component of ABC superfamily^[1], B1709^[2]^[1], BtuD^[2]^[1] , ECK1707, JW1699, b1709
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): btuCED^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name	btuD
Mnemonic	B12 uptake
Synonyms	ECK1707, b1709, JW1699^[1], JW1699
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	38.6 minutes	MG1655: 1791582..1790833 <gbrowseImage> name=NC_000913:1790833..1791582 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1770825..1770076 <gbrowseImage> name=NC_012967:1770076..1770825 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1682892..1683641 <gbrowseImage> name=NC_012759:1682892..1683641 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1795272..1794523 <gbrowseImage> name=NC_007779:1794523..1795272 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1882153..1881404 <gbrowseImage> name=NC_010473:1881404..1882153 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔbtuD (Keio:JW1699)	deletion	deletion	PMID:16738554	Shigen CGSC9438^[5]
btuD::Tn5KAN-2 (FB20435)	Insertion at nt 105 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20435	contains pKD46
ΔbtuD783::kan			PMID:16738554	CGSC:100216
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Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW1699	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTATTGTGATGCAGTTACA Primer 2:CCGATGGTCGAAATCAGCATTCT
20F5	Kohara Phage	Genobase	PMID:3038334
20H8	Kohara Phage	Genobase	PMID:3038334
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 62% ^[6] Synonyms:zdh-925::Tn10
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:zdi-276::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc	EcoCyc:EG10128	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10128	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000124	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945751	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0126	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005707	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BtuD
Synonyms	vitamin B12 transporter subunit : ATP-binding component of ABC superfamily^[1], B1709^[2]^[1], BtuD^[2]^[1] , ECK1707, JW1699, b1709
Product description	BtuD^[2]^[3]; Component of vitamin B12 ABC transporter^[2]^[3]; vitamin B12 transport system^[3] B12 uptake, ABC transporter permease^[4]
EC number (for enzymes)	3.6.3.33^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003593	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003439	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017871	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01005	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015420	cobalamin-transporting ATPase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01005	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000300	peripheral to membrane of membrane fraction	PMID:3528128^[7]	IDA: Inferred from Direct Assay		C		complete
	GO:0015420	cobalamin-transporting ATPase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.6.3.33	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015889	cobalamin transport	GO_REF:0000020	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0015889	cobalamin transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01005	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015889	cobalamin transport	GOA:hamap GO_REF:0000020	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016887	ATPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003439	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016887	ATPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017871	F	Seeded from EcoCyc (v14.0)	complete
	GO:0017111	nucleoside-triphosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003593	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of vitamin B12 ABC transporter	could be indirect
Protein	yfjI		PMID:16606699^[8]	Experiment(s):EBI-1140564
Protein	groL		PMID:16606699^[8]	Experiment(s):EBI-1140564
Protein	Subunits of vitamin B12 transport system	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSIVMQLQDV AESTRLGPLS GEVRAGEILH LVGPNGAGKS TLLARMAGMT SGKGSIQFAG QPLEAWSATK LALHRAYLSQ QQTPPFATPV WHYLTLHQHD KTRTELLNDV AGALALDDKL GRSTNQLSGG EWQRVRLAAV VLQITPQANP AGQLLLLDEP MNSLDVAQQS ALDKILSALC QQGLAIVMSS HDLNHTLRHA HRAWLLKGGK MLASGRREEV LTPPNLAQAY GMNFRRLDIE GHRMLISTI
Length	249
Mol. Wt	27.081 kDa
pI	9.5 (calculated)
Extinction coefficient	26,470 - 26,595 (calc based on 3 Y, 4 W, and 1 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
Domain	40..162	PF00005 ABC transporter		PMID:19920124^[9]
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<motif_map/>

Structure
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Structures

<beststructure> gene=btuD taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129665	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945751	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005707	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P06611	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10128	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10128	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945751	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000124	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0126	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	112	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	92	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	49^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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Transcription unit(s)
btuCED
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Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:1791562..1791602 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

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Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1709 (EcoliWiki Page)	NCBI GEO profiles for btuD
microarray	GenExpDB:b1709 (EcoliWiki Page)	Summary of data for btuD from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to btuD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10128	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0126	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1709
EcoGene	EcoGene:EG10128	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000124	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005707	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Shigella flexneri	BTUD	From SHIGELLACYC
E. coli O157	BTUD	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00005 ABC transporter
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Panther (EcoliWiki Page)	PTHR24220:SF143
EcoCyc	EcoCyc:EG10128	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10128	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000124	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0126	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005707	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ de Veaux, LC et al. (1986) Identification of the btuCED polypeptides and evidence for their role in vitamin B12 transport in Escherichia coli. J. Bacteriol. 167 920-7 PubMed
↑ ^8.0 ^8.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

btuD:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

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Sequence Features

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Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to btuD Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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