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Product(s) |

Expression|

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Standard Name	bisC
Gene Synonym(s)	ECK3538, b3551, JW5940^[1], JW5940
Product Desc.	biotin sulfoxide reductase^[2]^[3] Biotin sulfoxide and methionine (S) sulfoxide reductase; cytoplasmic^[4]
Product Synonyms(s)	biotin sulfoxide reductase^[1], B3551^[2]^[1], BisC^[2]^[1] , ECK3538, JW5940, b3551
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): bisC^[2]
Regulation/Activity
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Notes

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BisC is sufficient for growth on Met-S-SO. BisC is unable to act on protein bound Met-S-SO. The N-terminal 39 amino acids are required for activity and anchor BisC to the cytoplasmic side of the inner membrane.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	bisC
Mnemonic	Biotin sulfoxide
Synonyms	ECK3538, b3551, JW5940^[1], JW5940
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	80.01 minutes	MG1655: 3714363..3712084 <gbrowseImage> name=NC_000913:3712084..3714363 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3646798..3644519 <gbrowseImage> name=NC_012967:3644519..3646798 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3600410..3602743 <gbrowseImage> name=NC_012759:3600410..3602743 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3924075..3926354 <gbrowseImage> name=NC_007779:3924075..3926354 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3811940..3809661 <gbrowseImage> name=NC_010473:3809661..3811940 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
bisC(del) (Keio:JW5940)	deletion	deletion	PMID:16738554	Shigen
bisC::Tn5KAN-I-SceI (FB21274)	Insertion at nt 308 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21274	does not contain pKD46
bisC::Tn5KAN-I-SceI (FB21275)	Insertion at nt 308 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21275	contains pKD46
bisC9::Mu cts				CGSC:12224
bisC32				CGSC:73458
bisC10				CGSC:74336
bisC14				CGSC:74340
bisC20				CGSC:74341
bisC22				CGSC:74594
bisC33				CGSC:74710
bisC38				CGSC:74718
bisC39				CGSC:74720
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
9F6	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: % ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10124	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10124	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000120	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946915	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0122	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011597	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BisC
Synonyms	biotin sulfoxide reductase^[1], B3551^[2]^[1], BisC^[2]^[1] , ECK3538, JW5940, b3551
Product description	biotin sulfoxide reductase^[2]^[3] Biotin sulfoxide and methionine (S) sulfoxide reductase; cytoplasmic^[4]
EC number (for enzymes)	1.-.-.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0009055	electron carrier activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006655	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030151	molybdenum ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006657	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030151	molybdenum ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0500	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MENSLQSAVR DQVHSNTRVR FPMVRKGFLA SPENPQGIRG QDEFVRVSWD EALDLIHQQH KRIREAYGPA SIFAGSYGWR SNGVLHKAST LLQRYMALAG GYTGHLGDYS TGAAQAIMPY VVGGSEVYQQ QTSWPLVLEH SDVVVLWSAN PLNTLKIAWN ASDEQGLSYF SALRDSGKKL ICIDPMRSET VDFFGDKMEW VAPHMGTDVA LMLGIAHTLV ENGWHDEAFL ARCTTGYAVF ASYLLGESDG IAKTAEWAAE ICGVGAAKIR ELAAIFHQNT TMLMAGWGMQ RQQFGEQKHW MIVTLAAMLG QIGTPGGGFG LSYHFANGGN PTRRSAVLSS MQGSLPGGCD AVDKIPVARI VEALENPGGA YQHNGMNRHF PDIRFIWWAG GANFTHHQDT NRLIRAWQKP ELVVISECFW TAAAKHADIV LPATTSFERN DLTMTGDYSN QHLVPMKQVV PPRYEARNDF DVFAELSERW EKGGYARFTE GKSELQWLET FYNVARQRGA SQQVELPPFA EFWQANQLIE MPENPDSERF IRFADFCRDP LAHPLKTASG KIEIFSQRIA DYGYPDCPGH PMWLEPDEWQ GNAEPEQLQV LSAHPAHRLH SQLNYSSLRE LYAVANREPV TIHPDDAQER GIQDGDTVRL WNARGQILAG AVISEGIKPG VICIHEGAWP DLDLTADGIC KNGAVNVLTK DLPSSRLGNG CAGNTALAWL EKYNGPELTL TAFEPPASS
Length	739
Mol. Wt	81.91 kDa
pI	5.9 (calculated)
Extinction coefficient	152,290 - 153,540 (calc based on 21 Y, 22 W, and 10 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	56..517	PF00384 Molybdopterin oxidoreductase	PMID:19920124
Domain	636..754	PF01568 Molydopterin dinucleotide binding domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=bisC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:145698325	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946915	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011597	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P20099	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10124	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10124	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946915	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000120	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0122	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	9.99E+01	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	241	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	172	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	466	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
bisC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3714343..3714383 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3551 (EcoliWiki Page)	NCBI GEO profiles for bisC
microarray	GenExpDB:b3551 (EcoliWiki Page)	Summary of data for bisC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3713825..3714244) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:H3^[7]
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Notes

Accessions Related to bisC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10124	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0122	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3551
EcoGene	EcoGene:EG10124	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000120	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011597	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000012907 (score: 1.000; bootstrap: 87%)	From Inparanoid:20070104
Shigella flexneri	BISC	From SHIGELLACYC
E. coli O157	BISC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00384 Molybdopterin oxidoreductase
Pfam (EcoliWiki Page)	PF01568 Molydopterin dinucleotide binding domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:50692
Superfamily (EcoliWiki Page)	SUPERFAMILY:53706
EcoCyc	EcoCyc:EG10124	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10124	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000120	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0122	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011597	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

bisC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to bisC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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