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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	bglX
Gene Synonym(s)	ECK2125, b2132, JW2120, glh, yohA^[1], yohA
Product Desc.	β-D-glucoside glucohydrolase, periplasmic^[2]^[3] Periplasmic beta-glucosidase^[4]
Product Synonyms(s)	beta-D-glucoside glucohydrolase, periplasmic^[1], B2132^[2]^[1], YohA^[2]^[1], Glh^[2]^[1], BglX^[2]^[1] , ECK2125, glh, JW2120, yohA, b2132
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): bglX^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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First 20 aa are predicted to be a type I signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	bglX
Mnemonic	beta-Glucoside
Synonyms	ECK2125, b2132, JW2120, glh, yohA^[1], yohA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	47.8 minutes	MG1655: 2220011..2217714 <gbrowseImage> name=NC_000913:2217714..2220011 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2173227..2170930 <gbrowseImage> name=NC_012967:2170930..2173227 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2110197..2112494 <gbrowseImage> name=NC_012759:2110197..2112494 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2225323..2223026 <gbrowseImage> name=NC_007779:2223026..2225323 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2311019..2308722 <gbrowseImage> name=NC_010473:2308722..2311019 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔbglX (Keio:JW2120)	deletion	deletion	PMID:16738554	Shigen CGSC9720^[5]
bglX::Tn5KAN-2 (FB20631)	Insertion at nt 282 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20631	contains pKD46
bglX::Tn5KAN-2 (FB20632)	Insertion at nt 282 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20632	does not contain pKD46
ΔbglX758::kan			PMID:16738554	CGSC:100192
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2120	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAATGGCTATGTTCAGTAGG Primer 2:CCaAGCAACTCAAACTCGCCTTT
1E1	Kohara Phage	Genobase	PMID:3038334
2B2	Kohara Phage	Genobase	PMID:3038334
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
napA722::Tn10	Linked marker	CAG12098 = CGSC7400^[5]	est. P1 cotransduction: % ^[6] Synonyms:zeg-722, zei-722::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12013	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12013	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001932	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946682	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1951	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007043	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BglX
Synonyms	beta-D-glucoside glucohydrolase, periplasmic^[1], B2132^[2]^[1], YohA^[2]^[1], Glh^[2]^[1], BglX^[2]^[1] , ECK2125, glh, JW2120, yohA, b2132
Product description	β-D-glucoside glucohydrolase, periplasmic^[2]^[3] Periplasmic beta-glucosidase^[4]
EC number (for enzymes)	3.2.1.21^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001764	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002772	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019800	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001764	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002772	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019800	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID:8757730	IDA: Inferred from Direct Assay		C	isolated BglX from periplasmic extracts, data not shown in reference	complete
	GO:0008422	beta-glucosidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.2.1.21	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008422	beta-glucosidase activity	PMID:8757730	IDA: Inferred from Direct Assay		F	assay using ONP-beta-D-glucopyranoside	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015926	glucosidase activity	PMID:8757730	IDA: Inferred from Direct Assay		F	assay using ONP-beta-D-glucopyranoside	complete
	GO:0016798	hydrolase activity, acting on glycosyl bonds	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	ycgE	PMID:16606699	Experiment(s):EBI-1141998
Protein	ynaE	PMID:16606699	Experiment(s):EBI-1141998
Protein	rplB	PMID:16606699	Experiment(s):EBI-1141998
Protein	ydfK	PMID:16606699	Experiment(s):EBI-1141998
Protein	rplF	PMID:16606699	Experiment(s):EBI-1141998
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm
Periplasm	PMID:8757730	EchoLocation:bglX
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKWLCSVGIA VSLALQPALA DDLFGNHPLT PEARDAFVTE LLKKMTVDEK IGQLRLISVG PDNPKEAIRE MIKDGQVGAI FNTVTRQDIR AMQDQVMELS RLKIPLFFAY DVLHGQRTVF PISLGLASSF NLDAVKTVGR VSAYEAADDG LNMTWAPMVD VSRDPRWGRA SEGFGEDTYL TSTMGKTMVE AMQGKSPADR YSVMTSVKHF AAYGAVEGGK EYNTVDMSPQ RLFNDYMPPY KAGLDAGSGA VMVALNSLNG TPATSDSWLL KDVLRDQWGF KGITVSDHGA IKELIKHGTA ADPEDAVRVA LKSGINMSMS DEYYSKYLPG LIKSGKVTMA ELDDAARHVL NVKYDMGLFN DPYSHLGPKE SDPVDTNAES RLHRKEAREV ARESLVLLKN RLETLPLKKS ATIAVVGPLA DSKRDVMGSW SAAGVADQSV TVLTGIKNAV GENGKVLYAK GANVTSDKGI IDFLNQYEEA VKVDPRSPQE MIDEAVQTAK QSDVVVAVVG EAQGMAHEAS SRTDITIPQS QRDLIAALKA TGKPLVLVLM NGRPLALVKE DQQADAILET WFAGTEGGNA IADVLFGDYN PSGKLPMSFP RSVGQIPVYY SHLNTGRPYN ADKPNKYTSR YFDEANGALY PFGYGLSYTT FTVSDVKLSA PTMKRDGKVT ASVQVTNTGK REGATVVQMY LQDVTASMSR PVKQLKGFEK ITLKPGETQT VSFPIDIEAL KFWNQQMKYD AEPGKFNVFI GTDSARVKKG EFELL
Length	765
Mol. Wt	83.46 kDa
pI	6.1 (calculated)
Extinction coefficient	82,740 - 82,865 (calc based on 26 Y, 8 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-20	UniProt Manual:Signal Peptides	UniProt:P33363
Domain	96..320	PF00933 Glycosyl hydrolase family 3 N terminal domain		PMID:19920124
Domain	395..650	PF01915 Glycosyl hydrolase family 3 C terminal domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=bglX taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130070	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946682	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007043	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P33363	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12013	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12013	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946682	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001932	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1951	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.68E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	769	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	227	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	584	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
bglX
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2219991..2220031 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2132 (EcoliWiki Page)	NCBI GEO profiles for bglX
microarray	GenExpDB:b2132 (EcoliWiki Page)	Summary of data for bglX from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to bglX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12013	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1951	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2132
EcoGene	EcoGene:EG12013	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001932	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007043	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G02640 (score: 1.000; bootstrap: 54%) AT5G09730 (score: 1.000; bootstrap: 51%) AT5G64570 (score: 0.744) AT5G49360 (score: 0.598) AT3G19620 (score: 0.439) AT5G10560 (score: 0.364) AT1G78060 (score: 0.362) AT5G09700 (score: 0.315)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0215373 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000001155 (score: 1.000; bootstrap: 66%)	From Inparanoid:20070104
Oryza gramene	Q6VB92 (score: 1.000; bootstrap: 51%) Q8W2Z9 (score: 0.755) Q9LLB8 (score: 0.618) Q8W2Z8 (score: 0.612) Q42835 (score: 0.602) Q8RWR5 (score: 0.597) Q6PQF3 (score: 0.597) Q6ZG89 (score: 0.581) Q8W2Z2 (score: 0.548) Q9XEI3 (score: 0.546) Q9XE93 (score: 0.543) Q94ED2 (score: 0.541) Q60EH3 (score: 0.134)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC16834 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000009549 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	BGLX	From SHIGELLACYC
E. coli O157	BGLX	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01915 Glycosyl hydrolase family 3 C terminal domain
Pfam (EcoliWiki Page)	PF00933 Glycosyl hydrolase family 3 N terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:51445
Superfamily (EcoliWiki Page)	SUPERFAMILY:52279
EcoCyc	EcoCyc:EG12013	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12013	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001932	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1951	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007043	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

bglX:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to bglX Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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