bfr:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

bfr

Mnemonic

Bacterioferritin

Synonyms

ECK3323, b3336, JW3298[1], JW3298

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.67 minutes, 74.67 minutes 

MG1655: 3464747..3464271
<gbrowseImage> name=NC_000913:3464271..3464747 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3394630..3394154
<gbrowseImage> name=NC_012967:3394154..3394630 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3351428..3351904
<gbrowseImage> name=NC_012759:3351428..3351904 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4173691..4174167
<gbrowseImage> name=NC_007779:4173691..4174167 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3562492..3562016
<gbrowseImage> name=NC_010473:3562016..3562492 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3464271

Edman degradation

PMID:2644932[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

bfrN78G

N78G

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrR57K

R57K

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrL68I

L68I

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrAQIREEG152SQIKVKD

AQIREEG152SQIKVKD

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrR88Q

R88Q

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrA92R

A92R

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrD96E

D96E

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrG106A

G106A

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrR125A

R125A

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrN100D

N100D

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrTKV5VKI

TKV5VKI

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfrK38M

K38M

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

Δbfr (Keio:JW3298)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10467[4]

bfrQKM142GKI

QKM142GKI

(in strain: ECOR 30)

Strain variation; seeded from UniProt:P0ABD3

bfr::Tn5KAN-I-SceI (FB21118)

Insertion at nt 178 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21118

contains pKD46

bfr::Tn5KAN-I-SceI (FB21119)

Insertion at nt 178 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21119

does not contain pKD46

Δbfr-746::kan

PMID:16738554[3]

CGSC:100182


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3298

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAGGTGATACTAAAGTTAT

Primer 2:CCACCTTCTTCGCGGATCTGTGC

9H3

Kohara Phage

Genobase

PMID:3038334[7]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[4]

est. P1 cotransduction: 23% [8]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 20% [8]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10113

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10113

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000109

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947839

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0111

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010903

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Andrews, SC et al. (1989) Amino acid sequence of the bacterioferritin (cytochrome b1) of Escherichia coli-K12. Biochem. Biophys. Res. Commun. 158 489-96 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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