barA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
barA |
---|---|
Gene Synonym(s) | |
Product Desc. |
hybrid sensory histidine kinase, in two-component regulatory system with UvrY[1] Sensor histidine protein kinase, pleiotropic; controls the expresion of csrB/C sRNAs; works in concert with UvrY response regulator[3] |
Product Synonyms(s) | |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
BarA has both sensory kinase and response regulator domains. BarA is required for catalase (KatE) expression and resistance to hydrogen peroxide, through rpoS regulation.[3]. BarA is activated by short chain carbxylates.
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
barA |
---|---|
Mnemonic |
Bacterial adaptive response |
Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
62.79 minutes |
MG1655: 2913079..2915835 |
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NC_012967: 2803985..2806741 |
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NC_012759: 2800227..2802983 |
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W3110 |
|
W3110: 2913713..2916469 |
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DH10B: 3006957..3009713 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
barA(del) (Keio:JW2757) |
deletion |
deletion |
PMID:16738554 |
||||
barA::Tn5KAN-I-SceI (FB20927) |
Insertion at nt 1724 in Plus orientation |
PMID:15262929 |
contains pKD46 | ||||
barA::Tn5KAN-I-SceI (FB20928) |
Insertion at nt 1724 in Plus orientation |
PMID:15262929 |
does not contain pKD46 | ||||
barA1358(del) |
|||||||
barA614(del) |
PMID:12897016 |
||||||
barA784(del)::kan |
PMID:16738554 |
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barA784(del)::FRT |
Mutagenesis rate |
Decreased stress-induced mutagenesis (SIM) phenotype. |
PMID:23224554 |
Parental Strain: SMR4562 Experimental Strain: SMR12253 |
Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population. | ||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW2757 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCACCAACTACAGCCTGCGCGC Primer 2:CCgCCGAGAATTTTGCTGGCTTC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 35% [6] | ||
Linked marker |
est. P1 cotransduction: 50% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001335 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB1341 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0009131 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
BarA |
---|---|
Synonyms | |
Product description |
hybrid sensory histidine kinase, in two-component regulatory system with UvrY[1] Sensor histidine protein kinase, pleiotropic; controls the expresion of csrB/C sRNAs; works in concert with UvrY response regulator[3] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0009927 |
histidine phosphotransfer kinase activity |
PMID:11022030 |
IDA: Inferred from Direct Assay |
F |
complete | |||
GO:0000155 |
two-component sensor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003661 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000155 |
two-component sensor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008207 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0902 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004673 |
protein histidine kinase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004673 |
protein histidine kinase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:2.7.13.3 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003660 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008207 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009082 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003594 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0046777 |
protein amino acid autophosphorylation |
PMID:11022030 |
IDA: Inferred from Direct Assay |
P |
Figure 3 |
complete | ||
GO:0006350 |
transcription |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0804 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006468 |
protein amino acid phosphorylation |
PMID:11022030 |
IDA: Inferred from Direct Assay |
P |
Figure 3 |
complete | ||
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003660 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042542 |
response to hydrogen peroxide |
PMID:11022030 |
IMP: Inferred from Mutant Phenotype |
P |
complete | |||
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003661 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009082 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003661 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0472 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003660 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0812 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016310 |
phosphorylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004358 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0018106 |
peptidyl-histidine phosphorylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005467 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions
See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE PLAVSTEYGM SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN FHLDPSSMQL GSNVPFPRQL TVTRDGDIMI LRTPIISESY SPDESPSSDA KNSQNMLGYI ALELDLKSVR LQQYKEIFIS SVMMLFCIGI ALIFGWRLMR DVTGPIRNMV NTVDRIRRGQ LDSRVEGFML GELDMLKNGI NSMAMSLAAY HEEMQHNIDQ ATSDLRETLE QMEIQNVELD LAKKRAQEAA RIKSEFLANM SHELRTPLNG VIGFTRLTLK TELTPTQRDH LNTIERSANN LLAIINDVLD FSKLEAGKLI LESIPFPLRS TLDEVVTLLA HSSHDKGLEL TLNIKSDVPD NVIGDPLRLQ QIITNLVGNA IKFTENGNID ILVEKRALSN TKVQIEVQIR DTGIGIPERD QSRLFQAFRQ ADASISRRHG GTGLGLVITQ KLVNEMGGDI SFHSQPNRGS TFWFHINLDL NPNIIIEGPS TQCLAGKRLA YVEPNSAAAQ CTLDILSETP LEVVYSPTFS ALPPAHYDMM LLGIAVTFRE PLTMQHERLA KAVSMTDFLM LALPCHAQVN AEKLKQDGIG ACLLKPLTPT RLLPALTEFC HHKQNTLLPV TDESKLAMTV MAVDDNPANL KLIGALLEDM VQHVELCDSG HQAVERAKQM PFDLILMDIQ MPDMDGIRAC ELIHQLPHQQ QTPVIAVTAH AMAGQKEKLL GAGMSDYLAK PIEEERLHNL LLRYKPGSGI SSRVVTPEVN EIVVNPNATL DWQLALRQAA GKTDLARDML QMLLDFLPEV RNKVEEQLVG ENPEGLVDLI HKLHGSCGYS GVPRMKNLCQ LIEQQLRSGT KEEDLEPELL ELLDEMDNVA REASKILG |
Length |
918 |
Mol. Wt |
102.456 kDa |
pI |
5.5 (calculated) |
Extinction coefficient |
37,360 - 38,610 (calc based on 14 Y, 3 W, and 10 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0009131 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120001335 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1341 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
2.179+/-0.059 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.011066763 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
141 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
56 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
59 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2913059..2913099
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for barA | |
microarray |
Summary of data for barA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
GFP Fusion |
Intergenic region (2912930..2913082) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
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Notes
Accessions Related to barA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1341 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001335 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0009131 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Apis mellifera |
|
From Inparanoid:20070104 |
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
BARA |
From SHIGELLACYC |
E. coli O157 |
BARA |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase |
||
PF09984 Uncharacterized signal transduction histidine kinase domain (DUF2222) |
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EcoCyc:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11367 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001335 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1341 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0009131 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 4.0 4.1 Pernestig, AK et al. (2001) Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli. J. Biol. Chem. 276 225-31 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
Categories
- TMHMM Prediction
- Genes in OpenBioSystems with Promoter Fusions
- Genes with homologs in Apis mellifera
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Dictyostelium discoideum
- Genes with homologs in Oryza gramene
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157