aspS:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aspS

Mnemonic

Aspartate

Synonyms

ECK1867, b1866, JW1855, tls[1], tls

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

41.96 minutes 

MG1655: 1948546..1946774
<gbrowseImage> name=NC_000913:1946774..1948546 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1929292..1927520
<gbrowseImage> name=NC_012967:1927520..1929292 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1838833..1840605
<gbrowseImage> name=NC_012759:1838833..1840605 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1952236..1950464
<gbrowseImage> name=NC_007779:1950464..1952236 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2039117..2037345
<gbrowseImage> name=NC_010473:2037345..2039117 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1946774

Edman degradation

PMID:2129559[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1855

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCCGTACAGAATATTGTGGACA

Primer 2:CCGTTATTCTCAGCCTTCTTCAC

20H4

Kohara Phage

Genobase

PMID:3038334[4]

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[5]

est. P1 cotransduction: 55% [6]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[5]

est. P1 cotransduction: 15% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10097

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10097

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000093

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946385

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0095

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006226

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Eriani, G et al. (1990) Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases. Nucleic Acids Res. 18 7109-18 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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