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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	aroB
Gene Synonym(s)	ECK3376, b3389, JW3352^[1], JW3352
Product Desc.	AROB^[2]^[3] 3-Dehydroquinate synthase^[4]
Product Synonyms(s)	3-dehydroquinate synthase^[1], B3389^[2]^[1], AroB^[2]^[1] , ECK3376, JW3352, b3389
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aroKB-damX-dam-rpe-gph-trpS^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aroB
Mnemonic	Aromatic
Synonyms	ECK3376, b3389, JW3352^[1], JW3352
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	75.77 minutes, 75.77 minutes	MG1655: 3516508..3515420 <gbrowseImage> name=NC_000913:3515420..3516508 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3447168..3446080 <gbrowseImage> name=NC_012967:3446080..3447168 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3402577..3403665 <gbrowseImage> name=NC_012759:3402577..3403665 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4121930..4123018 <gbrowseImage> name=NC_007779:4121930..4123018 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3614253..3613165 <gbrowseImage> name=NC_010473:3613165..3614253 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
aroB(del) (Keio:JW3352)	deletion	deletion	PMID:16738554	Shigen CGSC10501^[5]
aroB::Tn5KAN-I-SceI (FB21151)	Insertion at nt 150 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21151	contains pKD46
aroB::Tn5KAN-I-SceI (FB21152)	Insertion at nt 150 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21152	does not contain pKD46
aroB351				CGSC:8221
aroB15				CGSC:10900
aroB11				CGSC:11004
aroB724(del)::kan			PMID:16738554	CGSC:100060
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3352	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAGAGGATTGTCGTTACTCT Primer 2:CCgGCTGATTGACAATCGGCAAT
4D4	Kohara Phage	Genobase	PMID:3038334
6F9	Kohara Phage	Genobase	PMID:3038334
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 65% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
glgP721::Tn10	Linked marker	CAG18638 = CGSC7451^[5]	est. P1 cotransduction: 11% ^[6] Synonyms:zhg-3086::Tn10, zhh-21::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10074	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10074	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000070	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947927	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0072	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011067	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AroB
Synonyms	3-dehydroquinate synthase^[1], B3389^[2]^[1], AroB^[2]^[1] , ECK3376, JW3352, b3389
Product description	AROB^[2]^[3] 3-Dehydroquinate synthase^[4]
EC number (for enzymes)	4.6.1.3^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003856	3-dehydroquinate synthase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00110	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003856	3-dehydroquinate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002658	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003856	3-dehydroquinate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016037	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003856	3-dehydroquinate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.3.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00110	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016037	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008652	cellular amino acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0028	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016303	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00110	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016037	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0057	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016838	carbon-oxygen lyase activity, acting on phosphates	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016303	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050897	cobalt ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0170	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003856	3-dehydroquinate synthase activity	PMID:6386050	IDA: Inferred from Direct Assay		F		complete
	GO:0005737	cytoplasm	PMID:6386050	IDA: Inferred from Direct Assay		C		complete
	GO:0009073	aromatic amino acid family biosynthetic process	PMID:6386050	TAS: Traceable Author Statement		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	groL	PMID:16606699	Experiment(s):EBI-1145778
Protein	fdnH	PMID:16606699	Experiment(s):EBI-1145778
Protein	yjhR	PMID:16606699	Experiment(s):EBI-1145778
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MERIVVTLGE RSYPITIASG LFNEPASFLP LKSGEQVMLV TNETLAPLYL DKVRGVLEQA GVNVDSVILP DGEQYKSLAV LDTVFTALLQ KPHGRDTTLV ALGGGVVGDL TGFAAASYQR GVRFIQVPTT LLSQVDSSVG GKTAVNHPLG KNMIGAFYQP ASVVVDLDCL KTLPPRELAS GLAEVIKYGI ILDGAFFNWL EENLDALLRL DGPAMAYCIR RCCELKAEVV AADERETGLR ALLNLGHTFG HAIEAEMGYG NWLHGEAVAA GMVMAARTSE RLGQFSSAET QRIITLLKRA GLPVNGPREM SAQAYLPHML RDKKVLAGEM RLILPLAIGK SEVRSGVSHE LVLNAIADCQ SA
Length	362
Mol. Wt	38.881 kDa
pI	6.0 (calculated)
Extinction coefficient	24,410 - 25,035 (calc based on 9 Y, 2 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	17..326	PF01761 3-dehydroquinate synthase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aroB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131267	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947927	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011067	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P07639	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10074	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10074	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947927	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000070	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0072	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	31.075+/-0.216	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.223805147	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	770	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	444	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	516	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aroKB-damX-dam-rpe-gph-trpS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3516488..3516528 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3389 (EcoliWiki Page)	NCBI GEO profiles for aroB
microarray	GenExpDB:b3389 (EcoliWiki Page)	Summary of data for aroB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aroB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10074	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0072	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3389
EcoGene	EcoGene:EG10074	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000070	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011067	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G66120 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000012442 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000014538 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q651F0 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000026460 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	AROB	From SHIGELLACYC
E. coli O157	AROB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01761 3-dehydroquinate synthase
Panther (EcoliWiki Page)	PTHR21090:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:56796
EcoCyc	EcoCyc:EG10074	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10074	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000070	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0072	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011067	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

aroB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aroB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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