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Gene |

Product(s) |

Expression|

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Standard Name	aroA
Gene Synonym(s)	ECK0899, b0908, JW0891^[1], JW0891
Product Desc.	AROA^[2]^[3] 5-enolpyruvyl shikimate-3-phosphate synthase; ESPS synthase; 3-phosphoshikimate-1-carboxyvinyltransferase^[4]
Product Synonyms(s)	5-enolpyruvylshikimate-3-phosphate synthetase^[1], B0908^[2]^[1], AroA^[2]^[1] , ECK0899, JW0891, b0908
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): serC-aroA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aroA
Mnemonic	Aromatic
Synonyms	ECK0899, b0908, JW0891^[1], JW0891
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	20.65 minutes	MG1655: 958035..959318 <gbrowseImage> name=NC_000913:958035..959318 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 975904..977187 <gbrowseImage> name=NC_012967:975904..977187 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 861003..862286 <gbrowseImage> name=NC_012759:861003..862286 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 959234..960517 <gbrowseImage> name=NC_007779:959234..960517 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1011963..1013246 <gbrowseImage> name=NC_010473:1011963..1013246 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
aroAG96A		G96A	(confers glyphosate inhibition)			Strain variation; seeded from UniProt:P0A6D3
aroA(del) (Keio:JW0891)	deletion	deletion		PMID:16738554	Shigen CGSC8914^[5]
aroA::Tn5KAN-2 (FB20249)	Insertion at nt 265 in Minus orientation			PMID:15262929	E. coli Genome Project:FB20249	does not contain pKD46
aroA354					CGSC:8214
aroA2					CGSC:9085
aroA357					CGSC:9912
aroA273::Tn10					CGSC:10270
aroA0					CGSC:74065
aroA732(del)::kan				PMID:16738554	CGSC:100058
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0891	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAATCCCTGACGTTACAACC Primer 2:CCGGCTGCCTGGCTAATCCGCGC
13E3	Kohara Phage	Genobase	PMID:3038334
6D12	Kohara Phage	Genobase	PMID:3038334
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 67% ^[6] Synonyms:zbj-1230::Tn10
zcb-3059::Tn10	Linked marker	CAG12094 = CGSC7358^[5]	est. P1 cotransduction: 14% ^[6]
MG1655aroA::Tn10	Strain		PMID:9721304
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10073	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10073	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000069	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945528	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0071	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003095	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AroA
Synonyms	5-enolpyruvylshikimate-3-phosphate synthetase^[1], B0908^[2]^[1], AroA^[2]^[1] , ECK0899, JW0891, b0908
Product description	AROA^[2]^[3] 5-enolpyruvyl shikimate-3-phosphate synthase; ESPS synthase; 3-phosphoshikimate-1-carboxyvinyltransferase^[4]
EC number (for enzymes)	2.5.1.19^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013792	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003866	3-phosphoshikimate 1-carboxyvinyltransferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00210	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003866	3-phosphoshikimate 1-carboxyvinyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006264	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003866	3-phosphoshikimate 1-carboxyvinyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016228	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003866	3-phosphoshikimate 1-carboxyvinyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.5.1.19	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00210	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016228	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008652	cellular amino acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0028	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00210	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016228	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0057	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001986	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	nadE	PMID:16606699	Experiment(s):EBI-1138324
Protein	hisP	PMID:16606699	Experiment(s):EBI-1138324
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE GDASSASYFL AAAAIKGGTV KVTGIGRNSM QGDIRFADVL EKMGATICWG DDYISCTRGE LNAIDMDMNH IPDAAMTIAT AALFAKGTTT LRNIYNWRVK ETDRLFAMAT ELRKVGAEVE EGHDYIRITP PEKLNFAEIA TYNDHRMAMC FSLVALSDTP VTILDPKCTA KTFPDYFEQL ARISQAA
Length	427
Mol. Wt	46.097 kDa
pI	5.4 (calculated)
Extinction coefficient	30,370 - 31,120 (calc based on 13 Y, 2 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	6..420	PF00275 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aroA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128875	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945528	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003095	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6D3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10073	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10073	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945528	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000069	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0071	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	6.73E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1248	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1636	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1018	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
serC-aroA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:958015..958055 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0908 (EcoliWiki Page)	NCBI GEO profiles for aroA
microarray	GenExpDB:b0908 (EcoliWiki Page)	Summary of data for aroA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aroA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10073	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0071	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0908
EcoGene	EcoGene:EG10073	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000069	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003095	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000025558 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT2G45300 (score: 1.000; bootstrap: 100%) AT1G48860 (score: 0.799)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000012252 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	O24566 (score: 1.000; bootstrap: 100%) Q95AK0 (score: 0.962) Q95AK1 (score: 0.946) Q93VK6 (score: 0.914) Q5VNW0 (score: 0.914) O80428 (score: 0.709) Q9AT37 (score: 0.494) Q6VVA4 (score: 0.296)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDR127W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC18342 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	AROA	From SHIGELLACYC
E. coli O157	AROA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00275 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Panther (EcoliWiki Page)	PTHR21090:SF5
Superfamily (EcoliWiki Page)	SUPERFAMILY:55205
EcoCyc	EcoCyc:EG10073	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10073	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000069	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0071	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003095	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

aroA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aroA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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