arcA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
arcA |
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Gene Synonym(s) |
ECK4393, b4401, JW4364, dye, fexA, msp, seg, sfrA, cpxC[1], cpxC |
Product Desc. |
ArcA transcriptional dual regulator[2][3] DNA-binding response regulator in two-component regulatory system with ArcB or CpxA product Response regulator, represses aerobic genes anaerobically; also activates some anaerobic genes; phosphorylated by ArcB[4] |
Product Synonyms(s) |
B4401 [2], negative response regulator of genes in aerobic pathways, (sensors, ArcB and CpxA) [2], CpxC [2], Dye [2], FexA [2], Msp [2], Seg [2], SfrA [2], respiration control protein ArcA [2], unphosphorylated respiration control protein ArcA [2], unmodified respiration control protein ArcA [2], ArcA control protein [2], dye resistance protein [2], aerobic respiration control protein ArcA [2] , cpxC, dye, ECK4393, fexA, JW4364, msp, seg, sfrA, b4401 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
arcA |
---|---|
Mnemonic |
Aerobic respiration control |
Synonyms |
ECK4393, b4401, JW4364, dye, fexA, msp, seg, sfrA, cpxC[1], cpxC |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
99.96 minutes |
MG1655: 4638329..4637613 |
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NC_012967: 4628466..4627750 |
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NC_012759: 4576097..4576813 |
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W3110 |
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W3110: 4644986..4644270 |
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DH10B: 4684791..4684075 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
4637613 |
Edman degradation |
PMID:9298646 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
arcA(del) (Keio:JW4364) |
deletion |
deletion |
PMID:16738554 |
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arcA43(del) |
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arcA45(del) |
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arcA44:(del):kan |
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arcA726(del)::kan |
PMID:16738554 |
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arcA |
mutation |
mutation |
represses aerobic function |
PMID:2565334 |
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arcA(P)::Tn10dCam |
Mutagenesis rate |
Decreased stress-induced mutagenesis (SIM) phenotype. |
PMID:23224554 |
Parental Strain: SMR4562 Experimental Strain: SMR12023 |
Mutation was comparatively weak with decrease only happening to 33-67% of population. | ||
CAG45114 arcA726(del)::FRTKanFRT |
deletion |
SigmaE Activity |
Decrease in SigmaE Activity |
PMID:23224554 |
Parental Strain: CAG45114 Experimental Strain:SMR1360 |
See Table S11 for experimental Data | |
CAG45114 arcA726(del)::FRTKanFRT |
Deletion |
SigmaE activity |
Increase in sigmaE activity |
PMID23224554 |
Parental Strain: CAG45114 Experimental Strain: SMR15360 |
See table S11 for full experimental data | |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW4364 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCCAGACCCCGCACATTCTTAT Primer 2:CCATCTTCCAGATCACCGCAGAA | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 14% [6] | ||
Linked marker |
est. P1 cotransduction: 93% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000057 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0059 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0014434 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
ArcA |
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Synonyms |
B4401 [2], negative response regulator of genes in aerobic pathways, (sensors, ArcB and CpxA) [2], CpxC [2], Dye [2], FexA [2], Msp [2], Seg [2], SfrA [2], respiration control protein ArcA [2], unphosphorylated respiration control protein ArcA [2], unmodified respiration control protein ArcA [2], ArcA control protein [2], dye resistance protein [2], aerobic respiration control protein ArcA [2] , cpxC, dye, ECK4393, fexA, JW4364, msp, seg, sfrA, b4401 |
Product description |
ArcA transcriptional dual regulator[2][3] DNA-binding response regulator in two-component regulatory system with ArcB or CpxA product Response regulator, represses aerobic genes anaerobically; also activates some anaerobic genes; phosphorylated by ArcB[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001867 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR016032 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
PMID:1512197 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001867 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR016032 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0902 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
PMID:1512197 |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003700 |
transcription factor activity |
PMID:14711822 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003700 |
transcription factor activity |
PMID:2964639 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003700 |
transcription factor activity |
PMID:2984198 |
ISS: Inferred from Sequence or Structural Similarity |
UniProtKB:P0AA16 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005622 |
intracellular |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR016032 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006355 |
regulation of transcription, DNA-dependent |
PMID:16849788 |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016563 |
transcription activator activity |
PMID:14711822 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016563 |
transcription activator activity |
PMID:2964639 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016563 |
transcription activator activity |
PMID:2984198 |
ISS: Inferred from Sequence or Structural Similarity |
UniProtKB:P0AA16 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016564 |
transcription repressor activity |
PMID:14711822 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016564 |
transcription repressor activity |
PMID:2964639 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016564 |
transcription repressor activity |
PMID:2984198 |
ISS: Inferred from Sequence or Structural Similarity |
UniProtKB:P0AA16 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0045892 |
negative regulation of transcription, DNA-dependent |
PMID:14711822 |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0045892 |
negative regulation of transcription, DNA-dependent |
PMID:2964639 |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0045892 |
negative regulation of transcription, DNA-dependent |
PMID:2984198 |
ISS: Inferred from Sequence or Structural Similarity |
UniProtKB:P0AA16 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0045893 |
positive regulation of transcription, DNA-dependent |
PMID:14711822 |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0045893 |
positive regulation of transcription, DNA-dependent |
PMID:2964639 |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0045893 |
positive regulation of transcription, DNA-dependent |
PMID:2984198 |
ISS: Inferred from Sequence or Structural Similarity |
UniProtKB:P0AA16 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions
See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
prs |
PMID:16606699 |
Experiment(s):EBI-1147992 | |
Protein |
ArcB |
ArcA and ArcB are cognate response regulator and sensor kinase, respectively. |
| |
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Notes
Phosphorylation required for DNA binding
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
cytoplasm |
From EcoCyc[3] |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MQTPHILIVE DELVTRNTLK SIFEAEGYDV FEATDGAEMH QILSEYDINL VIMDINLPGK NGLLLARELR EQANVALMFL TGRDNEVDKI LGLEIGADDY ITKPFNPREL TIRARNLLSR TMNLGTVSEE RRSVESYKFN GWELDINSRS LIGPDGEQYK LPRSEFRAML HFCENPGKIQ SRAELLKKMT GRELKPHDRT VDVTIRRIRK HFESTPDTPE IIATIHGEGY RFCGDLED |
Length |
238 |
Mol. Wt |
27.292 kDa |
pI |
5.1 (calculated) |
Extinction coefficient |
14,440 - 14,690 (calc based on 6 Y, 1 W, and 2 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0014434 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000057 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0059 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
2.66E+03 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
E. coli K-12 EMG2 |
200 |
molecules/cell |
|
Quantitative protein sequencing |
Spot ID: M118-I |
PMID: 9298646 |
Protein |
E. coli K-12 MG1655 |
8000 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
3622 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
4068 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:4638309..4638349
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
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This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for arcA | |
microarray |
Summary of data for arcA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to arcA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0059 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000057 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0014434 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
Shigella flexneri |
ARCA |
From SHIGELLACYC |
E. coli O157 |
ARCA |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10061 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000057 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0059 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0014434 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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