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Product(s) |

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Standard Name	appA
Gene Synonym(s)	ECK0971, b0980, JW0963^[1], JW0963
Product Desc.	AppA^[2]^[3] Acid phosphatase, pH 2.5, exopolyphosphatase; phytase^[4]
Product Synonyms(s)	phosphoanhydride phosphorylase^[1], B0980^[2]^[1], AppA^[2]^[1] , ECK0971, JW0963, b0980
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): appA^[2], appCBA^[2], OP00137, cyx-appA, cyxAB-appA
Regulation/Activity
Quick Links
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Notes

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EvgAS regulon. AppA requires DsbA for activity.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	appA
Mnemonic	Acid (poly)phosphatase
Synonyms	ECK0971, b0980, JW0963^[1], JW0963
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	22.41 minutes	MG1655: 1039840..1041138 <gbrowseImage> name=NC_000913:1039840..1041138 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1057708..1059006 <gbrowseImage> name=NC_012967:1057708..1059006 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 942808..944106 <gbrowseImage> name=NC_012759:942808..944106 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1041039..1042337 <gbrowseImage> name=NC_007779:1041039..1042337 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1093768..1095066 <gbrowseImage> name=NC_010473:1093768..1095066 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1039906	Edman degradation	PMID:2168385 PMID:8387749 PMID:10696472
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔappA (Keio:JW0963)	deletion	deletion	PMID:16738554	Shigen CGSC8958^[5]
ΔappA724::kan			PMID:16738554	CGSC:100016
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0963	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAGCGATCTTAATCCCATT Primer 2:CCCAAACTGCACGCCGGTATGCG
4H9	Kohara Phage	Genobase	PMID:3038334
4H11	Kohara Phage	Genobase	PMID:3038334
zcb-3059::Tn10	Linked marker	CAG12094 = CGSC7358^[5]	est. P1 cotransduction: 21% ^[6]
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: 43% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10049	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10049	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000045	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946206	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0047	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003305	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AppA
Synonyms	phosphoanhydride phosphorylase^[1], B0980^[2]^[1], AppA^[2]^[1] , ECK0971, JW0963, b0980
Product description	AppA^[2]^[3] Acid phosphatase, pH 2.5, exopolyphosphatase; phytase^[4]
EC number (for enzymes)	3.1.3.26^[1] 3.1.3.2^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0511	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003993	acid phosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003993	acid phosphatase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008707	4-phytase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.26	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	eaeH	PMID:16606699	Experiment(s):EBI-1138534
Protein	rplB	PMID:16606699	Experiment(s):EBI-1138534
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Periplasm	PMID:8387749	EchoLocation:appA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKAILIPFLS LLIPLTPQSA FAQSEPELKL ESVVIVSRHG VRAPTKATQL MQDVTPDAWP TWPVKLGWLT PRGGELIAYL GHYQRQRLVA DGLLAKKGCP QSGQVAIIAD VDERTRKTGE AFAAGLAPDC AITVHTQADT SSPDPLFNPL KTGVCQLDNA NVTDAILSRA GGSIADFTGH RQTAFRELER VLNFPQSNLC LKREKQDESC SLTQALPSEL KVSADNVSLT GAVSLASMLT EIFLLQQAQG MPEPGWGRIT DSHQWNTLLS LHNAQFYLLQ RTPEVARSRA TPLLDLIKTA LTPHPPQKQA YGVTLPTSVL FIAGHDTNLA NLGGALELNW TLPGQPDNTP PGGELVFERW RRLSDNSQWI QVSLVFQTLQ QMRDKTPLSL NTPPGEVKLT LAGCEERNAQ GMCSLAGFTQ IVNEARIPAC SL
Length	432
Mol. Wt	47.057 kDa
pI	6.7 (calculated)
Extinction coefficient	49,960 - 50,960 (calc based on 4 Y, 8 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P07102
Domain	29..377	PF00328 Histidine acid phosphatase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=appA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128946	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946206	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003305	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P07102	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10049	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10049	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946206	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000045	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0047	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	16	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	42	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
appA
appCBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1039820..1039860 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0980 (EcoliWiki Page)	NCBI GEO profiles for appA
microarray	GenExpDB:b0980 (EcoliWiki Page)	Summary of data for appA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1039557..1039883) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ06; Well:F11^[7]
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Notes

Accessions Related to appA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10049	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0047	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0980
EcoGene	EcoGene:EG10049	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000045	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003305	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000020951 (score: 1.000; bootstrap: 100%) ENSANGP00000014917 (score: 0.164) ENSANGP00000018380 (score: 0.143)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000004322 (score: 1.000; bootstrap: 100%) ENSCAFP00000012985 (score: 0.325) ENSCAFP00000009365 (score: 0.269)	From Inparanoid:20070104
Homo sapiens	ENSP00000270593 (score: 1.000; bootstrap: 100%) ENSP00000256997 (score: 0.328) ENSP00000323036 (score: 0.302) ENSP00000294912 (score: 0.058)	From Inparanoid:20070104
Mus musculus	MGI:3644563 (score: 1.000; bootstrap: 100%) MGI:87882 (score: 0.333) MGI:1928480 (score: 0.285)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000038370 (score: 1.000; bootstrap: 100%) ENSRNOP00000018620 (score: 0.339) ENSRNOP00000016222 (score: 0.296) ENSRNOP00000044125 (score: 0.053)	From Inparanoid:20070104
Shigella flexneri	APPA	From SHIGELLACYC
E. coli O157	APPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00328 Histidine acid phosphatase
Superfamily (EcoliWiki Page)	SUPERFAMILY:53254
EcoCyc	EcoCyc:EG10049	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10049	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000045	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0047	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003305	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

appA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to appA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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