allD:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

allD

Gene Synonym(s)

ECK0510, b0517, JW0505, glxB8, yibC, ylbC[1], ylbC

Product Desc.

G6286[2][3]

Ureidoglycolate dehydrogenase: allantoin for anaerobic nitrogen[4]

Product Synonyms(s)

ureidoglycolate dehydrogenase[1], B0517[2][1], YlbC[2][1], AllD[2][1] , ECK0510, glxB8, JW0505, ylbC, b0517

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): allDC-ylbA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

allD

Mnemonic

Allantoin

Synonyms

ECK0510, b0517, JW0505, glxB8, yibC, ylbC[1], ylbC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

11.74 minutes 

MG1655: 545587..544538
<gbrowseImage> name=NC_000913:544538..545587 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 518529..517480
<gbrowseImage> name=NC_012967:517480..518529 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 447297..448346
<gbrowseImage> name=NC_012759:447297..448346 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 545587..544538
<gbrowseImage> name=NC_007779:544538..545587 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 484918..483869
<gbrowseImage> name=NC_010473:483869..484918 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

allD(del) (Keio:JW0505)

deletion

deletion

PMID:16738554

Shigen

CGSC8649[5]

ylbC(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

allD729(del)::kan

PMID:16738554

CGSC:99976


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0505

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAATCAGTCGGGAAACACT

Primer 2:CCTTGCGCAAAGGGATTTTTCGT

6E7

Kohara Phage

Genobase

PMID:3038334

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[5]

est. P1 cotransduction: 15% [6]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[5]

est. P1 cotransduction: 77% [6]
Synonyms:purE79::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6286

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13624

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002894

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948342

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3389

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001780

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AllD

Synonyms

ureidoglycolate dehydrogenase[1], B0517[2][1], YlbC[2][1], AllD[2][1] , ECK0510, glxB8, JW0505, ylbC, b0517

Product description

G6286[2][3]

Ureidoglycolate dehydrogenase: allantoin for anaerobic nitrogen[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006144

purine base metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0659

P

Seeded from EcoCyc (v14.0)

complete

GO:0009040

ureidoglycolate dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.154

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003767

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Q79BD2

PMID:16606699

Experiment(s):EBI-1137002

Protein

groL

PMID:16606699

Experiment(s):EBI-1137002

Protein

uvrA

PMID:16606699

Experiment(s):EBI-1137002

Protein

nadE

PMID:16606699

Experiment(s):EBI-1137002

Protein

lacZ

PMID:16606699

Experiment(s):EBI-1137002

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKISRETLHQ LIENKLCQAG LKREHAATVA EVLVYADARG IHSHGAVRVE YYAERISKGG
TNREPEFRLE ETGPCSAILH ADNAAGQVAA KMGMEHAIKT AQQNGVAVVG ISRMGHSGAI
SYFVQQAARA GFIGISMCQS DPMVVPFGGA EIYYGTNPLA FAAPGEGDEI LTFDMATTVQ
AWGKVLDARS RNMSIPDTWA VDKNGVPTTD PFAVHALLPA AGPKGYGLMM MIDVLSGVLL
GLPFGRQVSS MYDDLHAGRN LGQLHIVINP NFFSSSELFR QHLSQTMREL NAITPAPGFN
QVYYPGQDQD IKQRKAAVEG IEIVDDIYQY LISDALYNTS YETKNPFAQ
Length

349

Mol. Wt

37.966 kDa

pI

6.1 (calculated)

Extinction coefficient

31,860 - 32,235 (calc based on 14 Y, 2 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..334

PF02615 Malate/L-lactate dehydrogenase

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=allD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128501

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948342

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001780

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77555

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6286

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13624

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948342

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002894

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3389

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

1a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

allDC-ylbA

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:545567..545607 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0517 (EcoliWiki Page)

NCBI GEO profiles for allD

microarray

GenExpDB:b0517 (EcoliWiki Page)

Summary of data for allD from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (545508..545928) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ13; Well:H7[7]

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Notes

Accessions Related to allD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6286

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3389

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0517

EcoGene

EcoGene:EG13624

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002894

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001780

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000025275 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000025105 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00026061 (score: 1.000; bootstrap: 100%)
  • WBGene00033328 (score: 0.377)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00012149 (score: 1.000; bootstrap: 100%)
  • WBGene00009453 (score: 0.390)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000009047 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0037057 (score: 1.000; bootstrap: 100%)
  • FBgn0042131 (score: 0.139)
  • FBgn0038775 (score: 0.135)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA10363-PA (score: 1.000; bootstrap: 100%)
  • GA15088-PA (score: 0.198)
  • GA14384-PA (score: 0.187)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000130372 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00016104001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000009589 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

E. coli O157

YLBC

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02615 Malate/L-lactate dehydrogenase

Superfamily (EcoliWiki Page)

SUPERFAMILY:89733

EcoCyc

EcoCyc:G6286

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13624

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002894

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3389

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001780

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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