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Expression|

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Standard Name	agaS
Gene Synonym(s)	ECK3124, b3136, JW3105, yraB^[1], yraB
Product Desc.	putative tagatose-6-phosphate aldose/ketose isomerase^[2]^[3] Tagatose-6-phosphate ketose/aldose isomerase^[4]
Product Synonyms(s)	tagatose-6-phosphate ketose/aldose isomerase^[1], B3136^[2]^[1], YraB^[2]^[1], AgaS^[2]^[1] , ECK3124, JW3105, yraB, b3136
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): agaSYBCDI^[2], agaSkbaYagaBCDI
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	agaS
Mnemonic	Acetylgalactosamine
Synonyms	ECK3124, b3136, JW3105, yraB^[1], yraB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	70.69 minutes	MG1655: 3279998..3281152 <gbrowseImage> name=NC_000913:3279998..3281152 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3217209..3218363 <gbrowseImage> name=NC_012967:3217209..3218363 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3167146..3168300 <gbrowseImage> name=NC_012759:3167146..3168300 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3281831..3282985 <gbrowseImage> name=NC_007779:3281831..3282985 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3377743..3378897 <gbrowseImage> name=NC_010473:3377743..3378897 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔagaS (Keio:JW3105)	deletion	deletion	PMID:16738554	Shigen CGSC10365^[5]
agaS::Tn5KAN-I-SceI (FB21037)	Insertion at nt 483 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21037	contains pKD46
ΔagaS750::kan			PMID:16738554	CGSC:99946
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3105	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCAGAAAATTACACCCCTGC Primer 2:CCTGCCTGCCACGGATGAATGAT
20F4	Kohara Phage	Genobase	PMID:3038334
16C4	Kohara Phage	Genobase	PMID:3038334
aer-3075::Tn10	Linked marker	CAG12152 = CGSC7438^[5]	est. P1 cotransduction: 3% ^[6] Synonyms:zgh-3075::Tn10, zgj-3075::Tn10
sfsB203::Tn10	Linked marker	CAG12072 = CGSC7440^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zgj-203::Tn10, zha-203::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7634	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12767	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004184	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947645	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2620	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010305	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AgaS
Synonyms	tagatose-6-phosphate ketose/aldose isomerase^[1], B3136^[2]^[1], YraB^[2]^[1], AgaS^[2]^[1] , ECK3124, JW3105, yraB, b3136
Product description	putative tagatose-6-phosphate aldose/ketose isomerase^[2]^[3] Tagatose-6-phosphate ketose/aldose isomerase^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005529	sugar binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001347	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001347	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016853	isomerase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0413	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016861	intramolecular oxidoreductase activity, interconverting aldoses and ketoses	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014180	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	cyaA	PMID:16606699	Experiment(s):EBI-1144880
Protein	nadE	PMID:16606699	Experiment(s):EBI-1144880
Protein	matC	PMID:16606699	Experiment(s):EBI-1144880
Protein	htpG	PMID:16606699	Experiment(s):EBI-1144880
Protein	yjhG	PMID:16606699	Experiment(s):EBI-1144880
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPENYTPAAA ATGTWTEEEI RHQPRAWIRS LTNIDALRSA LNNFLEPLLR KENLRIILTG AGTSAFIGDI IAPWLASHTG KNFSAVPTTD LVTNPMDYLN PAHPLLLISF GRSGNSPESV AAVELANQFV PECYHLPITC NEAGALYQNA INSDNAFALL MPAETHDRGF AMTSSITTMM ASCLAVFAPE TINSQTFRDV ADRCQAILTS LGDFSEGVFG YAPWKRIVYL GSGGLQGAAR ESALKVLELT AGKLAAFYDS PTGFRHGPKS LVDDETLVVV FVSSHPYTRQ YDLDLLAELR RDNQAMRVIA IAAESSDIVA AGPHIILPPS RHFIDVEQAF CFLMYAQTFA LMQSLHMGNT PDTPSASGTV NRVVQGVIIH PWQA
Length	384
Mol. Wt	41.782 kDa
pI	5.4 (calculated)
Extinction coefficient	42,400 - 43,025 (calc based on 10 Y, 5 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	48..189	PF01380 SIS domain	PMID:19920124
Domain	220..357	PF01380 SIS domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=agaS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131028	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947645	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010305	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P42907	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7634	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12767	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947645	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004184	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2620	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	11^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	3^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
agaSYBCDI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3279978..3280018 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3136 (EcoliWiki Page)	NCBI GEO profiles for agaS
microarray	GenExpDB:b3136 (EcoliWiki Page)	Summary of data for agaS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3279195..3279646) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ11; Well:D9^[7]
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Notes

Accessions Related to agaS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7634	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2620	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3136
EcoGene	EcoGene:EG12767	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004184	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010305	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:53697
Pfam (EcoliWiki Page)	PF01380 SIS domain
Pfam (EcoliWiki Page)	PF01380 SIS domain
EcoCyc	EcoCyc:G7634	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12767	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004184	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2620	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010305	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

agaS:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to agaS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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