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Gene |

Product(s) |

Expression|

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Standard Name	ade
Gene Synonym(s)	ECK3656, b3665, JW3640, yicP, adu, adeC(B.s.)^[1]^[2], adeC
Product Desc.	cryptic adenine deaminase^[2]^[3]; Component of cryptic adenine deaminase^[3] Cryptic (or inducer-unknown) adenine deaminase; can be activated by hns mutation or IS elements; adenine aminohydrolase^[4]
Product Synonyms(s)	cryptic adenine deaminase^[1], B3665^[2]^[1], Adu^[2]^[1], Ade^[2]^[1], YicP^[2]^[1] , adeC, adu, ECK3656, JW3640, yicP, b3665
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ade^[2]
Regulation/Activity
Quick Links
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Notes

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ade and the adajcent probable adenine permease gene yicO may constitute an inducible adenine utilization divergon, however the convergent gene arrangement is not conserved outside of E. coli.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ade
Mnemonic	Adenine DEaminase
Synonyms	ECK3656, b3665, JW3640, yicP, adu, adeC(B.s.)^[1]^[2], adeC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	82.81 minutes	MG1655: 3841987..3843753 <gbrowseImage> name=NC_000913:3841987..3843753 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3803896..3805662 <gbrowseImage> name=NC_012967:3803896..3805662 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3730320..3732086 <gbrowseImage> name=NC_012759:3730320..3732086 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3796451..3794685 <gbrowseImage> name=NC_007779:3794685..3796451 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3939571..3941337 <gbrowseImage> name=NC_010473:3939571..3941337 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
Δade (Keio:JW3640)	deletion	deletion	PMID:16738554	Shigen CGSC10671^[5]
yicP::Tn5KAN-2 (FB21378)	Insertion at nt 1566 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21378	does not contain pKD46
yicP::Tn5KAN-2 (FB21379)	Insertion at nt 1566 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21379	contains pKD46
Δade-770::kan			PMID:16738554	CGSC:99912
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3640	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAATTCTATTAACCATAA Primer 2:CCTTCCGTGACTTCCAGCGTAGT
1D11	Kohara Phage	Genobase	PMID:3038334
12D11	Kohara Phage	Genobase	PMID:3038334
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 61% ^[6]
zid-501::Tn10	Linked marker	CAG18499 = CGSC7458^[5]	est. P1 cotransduction: 28% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11692	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001635	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948210	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1643	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011979	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Ade
Synonyms	cryptic adenine deaminase^[1], B3665^[2]^[1], Adu^[2]^[1], Ade^[2]^[1], YicP^[2]^[1] , adeC, adu, ECK3656, JW3640, yicP, b3665
Product description	cryptic adenine deaminase^[2]^[3]; Component of cryptic adenine deaminase^[3] Cryptic (or inducer-unknown) adenine deaminase; can be activated by hns mutation or IS elements; adenine aminohydrolase^[4]
EC number (for enzymes)	3.5.4.2^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000034	adenine deaminase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01518	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000034	adenine deaminase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006679	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000034	adenine deaminase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.5.4.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006146	adenine catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006679	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011059	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0464	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000034	adenine deaminase activity	PMID:11440125	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0005625	soluble fraction	PMID:11440125	IDA: Inferred from Direct Assay		C		complete
	GO:0006146	adenine catabolic process	PMID:11440125	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0042803	protein homodimerization activity	PMID:11440125	IDA: Inferred from Direct Assay		F		complete
	GO:0030145	manganese ion binding	PMID:11440125	IDA: Inferred from Direct Assay		F		complete
	GO:0046101	hypoxanthine biosynthetic process	PMID:11440125	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of cryptic adenine deaminase	could be indirect
Protein	nadE		PMID:16606699	Experiment(s):EBI-1146450
Protein	nagC		PMID:16606699	Experiment(s):EBI-1146450
Protein	rimJ		PMID:16606699	Experiment(s):EBI-1146450
Protein	rplB		PMID:16606699	Experiment(s):EBI-1146450
Protein	yfaS		PMID:16606699	Experiment(s):EBI-1146450
Protein	rpoB		PMID:16606699	Experiment(s):EBI-1146450
Protein	ymfD		PMID:16606699	Experiment(s):EBI-1146450
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNNSINHKFH HISRAEYQEL LAVSRGDAVA DYIIDNVSIL DLINGGEISG PIVIKGRYIA GVGAEYTDAP ALQRIDARGA TAVPGFIDAH LHIESSMMTP VTFETATLPR GLTTVICDPH EIVNVMGEAG FAWFARCAEQ ARQNQYLQVS SCVPALEGCD VNGASFTLEQ MLAWRDHPQV TGLAEMMDYP GVISGQNALL DKLDAFRHLT LDGHCPGLGG KELNAYITAG IENCHESYQL EEGRRKLQLG MSLMIREGSA ARNLNALAPL INEFNSPQCM LCTDDRNPWE IAHEGHIDAL IRRLIEQHNV PLHVAYRVAS WSTARHFGLN HLGLLAPGKQ ADIVLLSDAR KVTVQQVLVK GEPIDAQTLQ AEESARLAQS APPYGNTIAR QPVSASDFAL QFTPGKRYRV IDVIHNELIT HSHSSVYSEN GFDRDDVSFI AVLERYGQRL APACGLLGGF GLNEGALAAT VSHDSHNIVV IGRSAEEMAL AVNQVIQDGG GLCVVRNGQV QSHLPLPIAG LMSTDTAQSL AEQIDALKAA ARECGPLPDE PFIQMAFLSL PVIPALKLTS QGLFDGEKFA FTTLEVTE
Length	588
Mol. Wt	63.739 kDa
pI	5.2 (calculated)
Extinction coefficient	41,370 - 42,745 (calc based on 13 Y, 4 W, and 11 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	81..347	PF01979 Amidohydrolase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ade taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131535	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948210	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011979	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31441	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11692	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11692	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948210	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001635	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1643	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	738	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	46	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	311	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ade
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3841967..3842007 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3665 (EcoliWiki Page)	NCBI GEO profiles for ade
microarray	GenExpDB:b3665 (EcoliWiki Page)	Summary of data for ade from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ade Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11692	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1643	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3665
EcoGene	EcoGene:EG11692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001635	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011979	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YICP	From SHIGELLACYC
E. coli O157	YICP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01979 Amidohydrolase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:51338
Superfamily (EcoliWiki Page)	SUPERFAMILY:51556
EcoCyc	EcoCyc:EG11692	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001635	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1643	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011979	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ade:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ade Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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