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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	acrA
Gene Synonym(s)	ECK0457, b0463, JW0452, Mb, lir, mbl, mtcA, sipB, nbsA^[1]
Product Desc.	AcrA Membrane Fusion Protein^[2]^[3]; Component of AcrAB-TolC Multidrug Efflux System^[2]; AcrAB-TolC Multidrug Efflux Transport System^[3] AcrAB-TolC multidrug efflux pump; additionally dye, detergent, solvent resistance; membrane-fusion lipoprotein^[4]
Product Synonyms(s)	multidrug efflux system^[1], B0463^[2]^[1], SipB^[2]^[1], Mbl^[2]^[1], Lir^[2]^[1], NbsA^[2]^[1], MtcA^[2]^[1], AcrA^[2]^[1], acridine efflux pump^[2]^[1] , ECK0457, JW0452, lir, Mb, mbl, mtcA, sipB, b0463
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): acrAB^[2]
Regulation/Activity
Quick Links
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Notes

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acrA was first identified as an acridine sensitive mutation. acrA is a mutational suppressor of topA deletion inviability in low osmolarity medium. AcrA is attached to the inner membrane. acrA is an acrE paralog. AcrA is trimeric. Mar regulon. First 24 aa are a type II signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	acrA
Mnemonic	Acridine
Synonyms	ECK0457, b0463, JW0452, Mb, lir, mbl, mtcA, sipB, nbsA^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	10.42 minutes	MG1655: 484843..483650 <gbrowseImage> name=NC_000913:483650..484843 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 457686..456493 <gbrowseImage> name=NC_012967:456493..457686 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 386409..387602 <gbrowseImage> name=NC_012759:386409..387602 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 484843..483650 <gbrowseImage> name=NC_007779:483650..484843 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 424174..422981 <gbrowseImage> name=NC_010473:422981..424174 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
acrA(del) (Keio:JW0452)	deletion	deletion			PMID:16738554	Shigen
acrA1						CGSC:6294
acrA13						CGSC:7201
acrA	deletion		Sensitivity to	decreases sensitivity to bicyclomycin	PMID:21357484		fig 2
acrA748(del)::FRT			Mutagenesis rate	Decreased stress-induced mutagenesis (SIM) phenotype.	PMID:23224554	Parental Strain: SMR4562 Experimental Strain: SMR12220	Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.
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Notes

AcrA mutants grow to higher cell density than wt ^[5]

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0452	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACAAAAACAGAGGGTTTAC Primer 2:CCAGACTTGGACTGTTCAGGCTG
19B7	Kohara Phage	Genobase	PMID:3038334
8C4	Kohara Phage	Genobase	PMID:3038334
zaj-3054::Tn10	Linked marker	CAG12017 = CGSC7339^[6]	est. P1 cotransduction: 40% ^[7] Synonyms:zba-3054::Tn10
gsk-3055::Tn10	Linked marker	CAG12154 = CGSC7341^[6]	est. P1 cotransduction: 54% ^[7] Synonyms:zbb-30-55::TnlO, zba-3055::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11703	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11703	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001646	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945112	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1654	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001603	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AcrA
Synonyms	multidrug efflux system^[1], B0463^[2]^[1], SipB^[2]^[1], Mbl^[2]^[1], Lir^[2]^[1], NbsA^[2]^[1], MtcA^[2]^[1], AcrA^[2]^[1], acridine efflux pump^[2]^[1] , ECK0457, JW0452, lir, Mb, mbl, mtcA, sipB, b0463
Product description	AcrA Membrane Fusion Protein^[2]^[3]; Component of AcrAB-TolC Multidrug Efflux System^[2]; AcrAB-TolC Multidrug Efflux Transport System^[3] AcrAB-TolC multidrug efflux pump; additionally dye, detergent, solvent resistance; membrane-fusion lipoprotein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:10920254	IPI: Inferred from Physical Interaction	UniProtKB:P31224	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042908	xenobiotic transport	PMID:8407802	IMP: Inferred from Mutant Phenotype		P	GO:0015238 drug transmembrane transporter activity (obsolete term) was replaced with GO:0042910 xenobiotic transmembrane transporter activity. GO:0042910 (molecular function term) replaced with GO:0042908 xenobiotic transport (biological process term) because genetic evidence not sufficient to infer molecular function.	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of AcrAB-TolC Multidrug Efflux System	could be indirect
Protein	eno		PMID:19402753	LCMS(ID Probability):99.6
Protein	gadB		PMID:19402753	LCMS(ID Probability):99.6
Protein	gapA		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
plasma membrane		From EcoCyc^[3]
Inner membrane	PMID:10894736	EchoLocation:acrA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNKNRGFTPL AVVLMLSGSL ALTGCDDKQA QQGGQQMPAV GVVTVKTEPL QITTELPGRT SAYRIAEVRP QVSGIILKRN FKEGSDIEAG VSLYQIDPAT YQATYDSAKG DLAKAQAAAN IAQLTVNRYQ KLLGTQYISK QEYDQALADA QQANAAVTAA KAAVETARIN LAYTKVTSPI SGRIGKSNVT EGALVQNGQA TALATVQQLD PIYVDVTQSS NDFLRLKQEL ANGTLKQENG KAKVSLITSD GIKFPQDGTL EFSDVTVDQT TGSITLRAIF PNPDHTLLPG MFVRARLEEG LNPNAILVPQ QGVTRTPRGD ATVLVVGADD KVETRPIVAS QAIGDKWLVT EGLKAGDRVV ISGLQKVRPG VQVKAQEVTA DNNQQAASGA QPEQSKS
Length	397
Mol. Wt	42.196 kDa
pI	8.2 (calculated)
Extinction coefficient	18,910 - 19,035 (calc based on 9 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-24	UniProt Manual:Signal Peptides	UniProt:P0AE06
Domain	64..361	PF00529 HlyD family secretion protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=acrA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128447	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945112	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001603	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AE06	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11703	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11703	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945112	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001646	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1654	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	6.32E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	3861	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1295	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2397	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
acrAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:484823..484863 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0463 (EcoliWiki Page)	NCBI GEO profiles for acrA
microarray	GenExpDB:b0463 (EcoliWiki Page)	Summary of data for acrA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to acrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11703	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1654	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0463
EcoGene	EcoGene:EG11703	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001646	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001603	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001354 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000051131 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ACRA	From SHIGELLACYC
E. coli O157	ACRA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00529 HlyD family secretion protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:111369
EcoCyc	EcoCyc:EG11703	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11703	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001646	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1654	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001603	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Yang, S et al. (2006) Quorum sensing and multidrug transporters in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 103 2386-91 PubMed
↑ ^6.0 ^6.1 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

acrA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to acrA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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