aceE:Gene
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
aceE |
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Mnemonic |
Acetate |
Synonyms |
ECK0113, b0114, JW0110[1], JW0110 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
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MG1655 |
2.65 minutes |
MG1655: 123017..125680 |
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NC_012967: 125821..128484 |
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NC_012759: 123016..125679 |
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W3110 |
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W3110: 123017..125680 |
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DH10B: 97121..99784 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
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Coding Start (SO:0000323) |
123020 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
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aceE(del) (Keio:JW0110) |
deletion |
deletion |
Auxotrophies |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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aceE(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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aceE64 |
Auxotrophies |
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aceE2 |
Auxotrophies |
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aceE732(del)::kan |
Auxotrophies |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
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JW0110 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCAGAACGTTTCCCAAATGA Primer 2:CCCGCCAGACGCGGGTTAACTTT | |
Kohara Phage |
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Kohara Phage |
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leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 19% [8] | |
Linked marker |
est. P1 cotransduction: 38% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
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Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).