aas:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

aas

Gene Synonym(s)

ECK2832, b2836, JW2804[1], JW2804

Product Desc.

AAS[2][3]

Acyl-ACP synthase; 2-acylglycerophosphoethanolamine acyltransferase; salvage pathway for reacylation; inner membrane; bifunctional for turnover/incorporation[4]

Product Synonyms(s)

fused 2-acylglycerophospho-ethanolamine acyl transferase[1], acyl-acyl carrier protein synthetase[1], B2836[2][1], Aas[2][1] , ECK2832, JW2804, b2836

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ygeD[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aas

Mnemonic

Acyl-ACP synthase

Synonyms

ECK2832, b2836, JW2804[1], JW2804

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

64.05 minutes 

MG1655: 2974036..2971877
<gbrowseImage> name=NC_000913:2971877..2974036 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2859025..2861184
<gbrowseImage> name=NC_012759:2859025..2861184 source=BW2952 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2870634..2868475
<gbrowseImage> name=NC_012967:2868475..2870634 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2974670..2972511
<gbrowseImage> name=NC_007779:2972511..2974670 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3067906..3065747
<gbrowseImage> name=NC_010473:3065747..3067906 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

aasH36A

H36A

Loss of 2-acyl-GPE acyltransferase activity; retains acyl-ACP synthetase activity

seeded from UniProt:P31119

Δaas (Keio:JW2804)

deletion

deletion

PMID:16738554

Shigen
CGSC10191[5]

aas::Tn5KAN-I-SceI (FB20955)

Insertion at nt 895 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20955

does not contain pKD46

aas::Tn5KAN-I-SceI (FB20956)

Insertion at nt 895 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20956

contains pKD46

Δaas-761::kan

PMID:16738554

CGSC:99872


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2804

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCTTTTTAGCTTTTTTCGAAA

Primer 2:CCtTCATCGTGTTGTTCCGCTTC

5A10

Kohara Phage

Genobase

PMID:3038334

8H3

Kohara Phage

Genobase

PMID:3038334

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[5]

est. P1 cotransduction: 46% [6]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[5]

est. P1 cotransduction: % [6]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11679

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11679

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001622

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947315

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1630

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009302

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Aas

Synonyms

fused 2-acylglycerophospho-ethanolamine acyl transferase[1], acyl-acyl carrier protein synthetase[1], B2836[2][1], Aas[2][1] , ECK2832, JW2804, b2836

Product description

AAS[2][3]

Acyl-ACP synthase; 2-acylglycerophosphoethanolamine acyltransferase; salvage pathway for reacylation; inner membrane; bifunctional for turnover/incorporation[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000873

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0511

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01162

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006631

fatty acid metabolic process

PMID:1649829

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0008415

acyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002123

F

Seeded from EcoCyc (v14.0)

complete

GO:0008415

acyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0012

F

Seeded from EcoCyc (v14.0)

complete

GO:0008654

phospholipid biosynthetic process

PMID:1649829

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0008779

acyl-(acyl-carrier-protein)-phospholipid O-acyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01162

F

Seeded from EcoCyc (v14.0)

complete

GO:0008779

acyl-(acyl-carrier-protein)-phospholipid O-acyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.3.1.40

F

Seeded from EcoCyc (v14.0)

complete

GO:0008922

long-chain fatty acid (acyl-carrier-protein) ligase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01162

F

Seeded from EcoCyc (v14.0)

complete

GO:0008922

long-chain fatty acid (acyl-carrier-protein) ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.2.1.20

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

PMID:378999

IDA: Inferred from Direct Assay

C

complete

GO:0008779

acyl-(acyl-carrier-protein)-phospholipid O-acyltransferase activity

PMID:378999

IDA: Inferred from Direct Assay

F

complete

GO:0008922

long-chain fatty acid (acyl-carrier-protein) ligase activity

PMID:378999

IDA: Inferred from Direct Assay

F

complete

GO:0008654

phospholipid biosynthetic process

PMID:1649829

IGI: Inferred from Genetic Interaction

EcoliWiki:fadD

P

complete

GO:0006631

fatty acid metabolic process

PMID:1649829

IGI: Inferred from Genetic Interaction

EcoliWiki:fadD

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

groL

PMID:16606699

Experiment(s):EBI-1144119

Protein

rpsM

PMID:16606699

Experiment(s):EBI-1144119
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLFSFFRNLC RVLYRVRVTG DTQALKGERV LITPNHVSFI DGILLGLFLP VRPVFAVYTS
ISQQWYMRWL KSFIDFVPLD PTQPMAIKHL VRLVEQGRPV VIFPEGRITT TGSLMKIYDG
AGFVAAKSGA TVIPVRIEGA ELTHFSRLKG LVKRRLFPQI TLHILPPTQV AMPDAPRARD
RRKIAGEMLH QIMMEARMAV RPRETLYESL LSAMYRFGAG KKCVEDVNFT PDSYRKLLTK
TLFVGRILEK YSVEGERIGL MLPNAGISAA VIFGAIARRR MPAMMNYTAG VKGLTSAITA
AEIKTIFTSR QFLDKGKLWH LPEQLTQVRW VYLEDLKADV TTADKVWIFA HLLMPRLAQV
KQQPEEEALI LFTSGSEGHP KGVVHSHKSI LANVEQIKTI ADFTTNDRFM SALPLFHSFG
LTVGLFTPLL TGAEVFLYPS PLHYRIVPEL VYDRSCTVLF GTSTFLGHYA RFANPYDFYR
LRYVVAGAEK LQESTKQLWQ DKFGLRILEG YGVTECAPVV SINVPMAAKP GTVGRILPGM
DARLLSVPGI EEGGRLQLKG PNIMNGYLRV EKPGVLEVPT AENVRGEMER GWYDTGDIVR
FDEQGFVQIQ GRAKRFAKIA GEMVSLEMVE QLALGVSPDK VHATAIKSDA SKGEALVLFT
TDNELTRDKL QQYAREHGVP ELAVPRDIRY LKQMPLLGSG KPDFVTLKSW VDEAEQHDE
Length

719

Mol. Wt

80.701 kDa

pI

9.7 (calculated)

Extinction coefficient

76,780 - 77,280 (calc based on 22 Y, 8 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

233..642

PF00501 AMP-binding enzyme

PMID:19920124

Domain

15..138

PF01553 Acyltransferase

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aas taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130740

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947315

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009302

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P31119

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11679

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11679

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947315

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001622

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1630

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

188

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

80

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

162

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ygeD

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2974016..2974056 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2836 (EcoliWiki Page)

NCBI GEO profiles for aas

microarray

GenExpDB:b2836 (EcoliWiki Page)

Summary of data for aas from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to aas Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11679

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1630

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2836

EcoGene

EcoGene:EG11679

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001622

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009302

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

AAS

From SHIGELLACYC

E. coli O157

AAS

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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Database Accession Notes

Pfam (EcoliWiki Page)

PF00501 AMP-binding enzyme

Pfam (EcoliWiki Page)

PF01553 Acyltransferase

Panther (EcoliWiki Page)

PTHR24096:SF19

Superfamily (EcoliWiki Page)

SUPERFAMILY:56801

Superfamily (EcoliWiki Page)

SUPERFAMILY:69593

PFAM (EcoliWiki Page)

PFAM:PF01553

EcoCyc

EcoCyc:EG11679

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11679

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001622

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1630

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009302

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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