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Expression|

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Standard Name	aaeA
Gene Synonym(s)	ECK3230, b3241, JW3210, yhcQ^[1], yhcQ
Product Desc.	AaeAB Hydroxylated, Aromatic Carboxylic Acid Efflux Transport System Protein A^[2]^[3]; Component of AaeAB Hydroxylated, Aromatic Carboxylic Acid Efflux Transport System^[3] AaeAB p-hydroxybenzoic acid efflux pump MFP component; membrane fusion protein^[4]
Product Synonyms(s)	p-hydroxybenzoic acid efflux system component^[1], B3241^[2]^[1], AaeA^[2]^[1], YhcQ^[2]^[1] , ECK3230, JW3210, yhcQ, b3241
Function from GO	<GO_nr />
Knock-Out Phenotype	Biological Process GO:0046942 carboxylic acid transport (IGI) GO:0006810 transport (IGI) GO:0042221 response to chemical stimulus (IDA)
Regulation/Expression
Regulation/Activity	transcription unit(s): aaeB^[2]
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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AaeAB also exports related aromatic carboxylic acids. The aaeA gene is paralogous to ydhJ. Mutants in aaeA(yhcQ) have antibiotic-sensitve biofilms, and may be down-regulated in hepA(rapA) mutants which have a similar phenotype (Lynch, 2007).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aaeA
Mnemonic	Aromatic (carboxylic) acid efflux
Synonyms	ECK3230, b3241, JW3210, yhcQ^[1], yhcQ
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	72.98 minutes	MG1655: 3387148..3386216 <gbrowseImage> name=NC_000913:3386216..3387148 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3316091..3317023 <gbrowseImage> name=NC_012967:3316091..3317023 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3273364..3274296 <gbrowseImage> name=NC_012759:3273364..3274296 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3388981..3388049 <gbrowseImage> name=NC_007779:3388049..3388981 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3484893..3483961 <gbrowseImage> name=NC_010473:3483961..3484893 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
aaeA(del) (Keio:JW3210)	deletion	deletion	PMID:16738554	Shigen CGSC10434^[5]
aaeA766(del)::kan			PMID:16738554	CGSC:99866
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3210	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAACACTAATAAGAAAATT Primer 2:CCACCAAACTCACGCAGGCGATG
3G9	Kohara Phage	Genobase	PMID:3038334
3G9	Kohara Phage	Genobase	PMID:3038334
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[5]	est. P1 cotransduction: 44% ^[6] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[5]	est. P1 cotransduction: 33% ^[6] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7686	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12823	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004233	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947748	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2674	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010631	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AaeA
Synonyms	p-hydroxybenzoic acid efflux system component^[1], B3241^[2]^[1], AaeA^[2]^[1], YhcQ^[2]^[1] , ECK3230, JW3210, yhcQ, b3241
Product description	AaeAB Hydroxylated, Aromatic Carboxylic Acid Efflux Transport System Protein A^[2]^[3]; Component of AaeAB Hydroxylated, Aromatic Carboxylic Acid Efflux Transport System^[3] AaeAB p-hydroxybenzoic acid efflux pump MFP component; membrane fusion protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01544	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01544	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046942	carboxylic acid transport	PMID:15489430	IGI: Inferred from Genetic Interaction	EcoliWiki:aaeB	P		complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	PMID:15489430	IGI: Inferred from Genetic Interaction	EcoliWiki:aaeB	P		complete
	GO:0006810	transport	PMID:15489430	IGI: Inferred from Genetic Interaction		P	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0008565	protein transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042221	response to chemical stimulus	PMID:15489430	IDA: Inferred from Direct Assay		P		complete
	GO:0009306	protein secretion	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9904	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046942	carboxylic acid transport	PMID:15489430	IGI: Inferred from Genetic Interaction		P	Seeded from EcoCyc (v14.0)	Missing: with/from
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of AaeAB Hydroxylated, Aromatic Carboxylic Acid Efflux Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Periplasm		PMID:15489430	EchoLocation:aaeA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKTLIRKFSR TAITVVLVIL AFIAIFNAWV YYTESPWTRD ARFSADVVAI APDVSGLITQ VNVHDNQLVK KGQILFTIDQ PRYQKALEEA QADVAYYQVL AQEKRQEAGR RNRLGVQAMS REEIDQANNV LQTVLHQLAK AQATRDLAKL DLERTVIRAP ADGWVTNLNV YTGEFITRGS TAVALVKQNS FYVLAYMEET KLEGVRPGYR AEITPLGSNK VLKGTVDSVA AGVTNASSTR DDKGMATIDS NLEWVRLAQR VPVRIRLDNQ QENIWPAGTT ATVVVTGKQD RDESQDSFFR KMAHRLREFG
Length	310
Mol. Wt	34.773 kDa
pI	9.7 (calculated)
Extinction coefficient	40,910 (calc based on 9 Y, 5 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	46..115	PF00529 HlyD family secretion protein	PMID:19920124
Domain	100..285	PF00529 HlyD family secretion protein	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aaeA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131131	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947748	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010631	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P46482	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7686	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12823	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947748	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004233	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2674	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	62	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	21^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aaeB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3387128..3387168 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3241 (EcoliWiki Page)	NCBI GEO profiles for aaeA
microarray	GenExpDB:b3241 (EcoliWiki Page)	Summary of data for aaeA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aaeA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7686	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2674	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3241
EcoGene	EcoGene:EG12823	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004233	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010631	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YHCQ	From SHIGELLACYC
E. coli O157	YHCQ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00529 HlyD family secretion protein
Pfam (EcoliWiki Page)	PF00529 HlyD family secretion protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:111369
EcoCyc	EcoCyc:G7686	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12823	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004233	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2674	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010631	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

aaeA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aaeA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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